Strain Fitness in Escherichia coli ECRC62 around BNILDI_06945

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntqueG and nnr are separated by 13 nucleotidesnnr and tsaE overlap by 29 nucleotidestsaE and amiB are separated by 18 nucleotides BNILDI_06940: queG - tRNA epoxyqueuosine(34) reductase QueG, at 1,387,670 to 1,388,809 queG BNILDI_06945: nnr - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 1,388,823 to 1,390,355 nnr BNILDI_06950: tsaE - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 1,390,327 to 1,390,788 tsaE BNILDI_06955: amiB - N-acetylmuramoyl-L-alanine amidase AmiB, at 1,390,807 to 1,392,144 amiB Position (kb) 1388 1389 1390 1391Strain fitness (log2 ratio) -3 -2 -1 0 1at 1387.868 kb on + strand, within queGat 1387.882 kb on + strand, within queGat 1387.883 kb on - strand, within queGat 1387.909 kb on + strand, within queGat 1387.967 kb on + strand, within queGat 1387.967 kb on + strand, within queGat 1387.992 kb on - strand, within queGat 1388.057 kb on - strand, within queGat 1388.057 kb on - strand, within queGat 1388.079 kb on + strand, within queGat 1388.079 kb on + strand, within queGat 1388.084 kb on - strand, within queGat 1388.146 kb on - strand, within queGat 1388.152 kb on + strand, within queGat 1388.152 kb on + strand, within queGat 1388.153 kb on - strand, within queGat 1388.173 kb on + strand, within queGat 1388.173 kb on + strand, within queGat 1388.173 kb on + strand, within queGat 1388.173 kb on + strand, within queGat 1388.336 kb on - strand, within queGat 1388.511 kb on + strand, within queGat 1388.511 kb on + strand, within queGat 1388.566 kb on - strand, within queGat 1388.725 kb on + strandat 1388.725 kb on + strandat 1388.728 kb on + strandat 1388.728 kb on + strandat 1388.729 kb on - strandat 1388.732 kb on + strandat 1388.732 kb on + strandat 1388.732 kb on + strandat 1388.732 kb on + strandat 1388.733 kb on - strandat 1388.733 kb on - strandat 1388.819 kb on + strandat 1388.819 kb on + strandat 1388.820 kb on - strandat 1388.820 kb on - strandat 1388.820 kb on - strandat 1388.843 kb on + strandat 1388.843 kb on + strandat 1388.844 kb on - strandat 1388.845 kb on + strandat 1388.915 kb on + strandat 1388.916 kb on - strandat 1388.916 kb on - strandat 1388.916 kb on - strandat 1388.922 kb on + strandat 1388.923 kb on - strandat 1389.145 kb on + strand, within nnrat 1389.176 kb on + strand, within nnrat 1389.278 kb on + strand, within nnrat 1389.621 kb on - strand, within nnrat 1389.640 kb on + strand, within nnrat 1389.750 kb on - strand, within nnrat 1389.955 kb on + strand, within nnrat 1389.955 kb on + strand, within nnrat 1389.956 kb on - strand, within nnrat 1389.992 kb on - strand, within nnrat 1390.012 kb on + strand, within nnrat 1390.145 kb on + strand, within nnrat 1390.182 kb on + strand, within nnrat 1390.183 kb on - strand, within nnrat 1390.288 kb on + strandat 1390.814 kb on - strandat 1390.899 kb on + strandat 1390.951 kb on + strand, within amiBat 1390.952 kb on - strand, within amiBat 1390.952 kb on - strand, within amiBat 1390.952 kb on - strand, within amiBat 1391.161 kb on + strand, within amiBat 1391.161 kb on + strand, within amiBat 1391.161 kb on + strand, within amiBat 1391.162 kb on - strand, within amiBat 1391.162 kb on - strand, within amiBat 1391.162 kb on - strand, within amiBat 1391.162 kb on - strand, within amiBat 1391.302 kb on - strand, within amiBat 1391.319 kb on + strand, within amiBat 1391.332 kb on - strand, within amiB

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,387,868 + queG BNILDI_06940 0.17 +0.3
1,387,882 + queG BNILDI_06940 0.19 -0.8
1,387,883 - queG BNILDI_06940 0.19 -0.1
1,387,909 + queG BNILDI_06940 0.21 +0.1
1,387,967 + queG BNILDI_06940 0.26 +0.4
1,387,967 + queG BNILDI_06940 0.26 -2.1
1,387,992 - queG BNILDI_06940 0.28 -0.9
1,388,057 - queG BNILDI_06940 0.34 -0.5
1,388,057 - queG BNILDI_06940 0.34 -0.1
1,388,079 + queG BNILDI_06940 0.36 -0.3
1,388,079 + queG BNILDI_06940 0.36 -0.4
1,388,084 - queG BNILDI_06940 0.36 +0.3
1,388,146 - queG BNILDI_06940 0.42 -1.2
1,388,152 + queG BNILDI_06940 0.42 -0.6
1,388,152 + queG BNILDI_06940 0.42 +1.1
1,388,153 - queG BNILDI_06940 0.42 -0.9
1,388,173 + queG BNILDI_06940 0.44 -2.4
1,388,173 + queG BNILDI_06940 0.44 -2.6
1,388,173 + queG BNILDI_06940 0.44 +0.3
1,388,173 + queG BNILDI_06940 0.44 -0.3
1,388,336 - queG BNILDI_06940 0.58 -1.9
1,388,511 + queG BNILDI_06940 0.74 +0.5
1,388,511 + queG BNILDI_06940 0.74 -0.1
1,388,566 - queG BNILDI_06940 0.79 +0.4
1,388,725 + -0.2
1,388,725 + -1.1
1,388,728 + +0.7
1,388,728 + -0.1
1,388,729 - -0.3
1,388,732 + -0.6
1,388,732 + +0.4
1,388,732 + -0.3
1,388,732 + -0.3
1,388,733 - -0.2
1,388,733 - -0.2
1,388,819 + +0.4
1,388,819 + +0.2
1,388,820 - -2.9
1,388,820 - -2.2
1,388,820 - +0.2
1,388,843 + -0.3
1,388,843 + +0.7
1,388,844 - -0.2
1,388,845 + +0.9
1,388,915 + +0.2
1,388,916 - +0.6
1,388,916 - -0.4
1,388,916 - -0.5
1,388,922 + +0.2
1,388,923 - -0.8
1,389,145 + nnr BNILDI_06945 0.21 -0.9
1,389,176 + nnr BNILDI_06945 0.23 +0.3
1,389,278 + nnr BNILDI_06945 0.30 -0.5
1,389,621 - nnr BNILDI_06945 0.52 +0.4
1,389,640 + nnr BNILDI_06945 0.53 -1.3
1,389,750 - nnr BNILDI_06945 0.60 -0.1
1,389,955 + nnr BNILDI_06945 0.74 +0.1
1,389,955 + nnr BNILDI_06945 0.74 +0.3
1,389,956 - nnr BNILDI_06945 0.74 +0.0
1,389,992 - nnr BNILDI_06945 0.76 +0.8
1,390,012 + nnr BNILDI_06945 0.78 -0.1
1,390,145 + nnr BNILDI_06945 0.86 -0.4
1,390,182 + nnr BNILDI_06945 0.89 -0.1
1,390,183 - nnr BNILDI_06945 0.89 +0.6
1,390,288 + -0.8
1,390,814 - +0.5
1,390,899 + +1.0
1,390,951 + amiB BNILDI_06955 0.11 +0.1
1,390,952 - amiB BNILDI_06955 0.11 +1.0
1,390,952 - amiB BNILDI_06955 0.11 +0.1
1,390,952 - amiB BNILDI_06955 0.11 +0.0
1,391,161 + amiB BNILDI_06955 0.26 -0.1
1,391,161 + amiB BNILDI_06955 0.26 +1.7
1,391,161 + amiB BNILDI_06955 0.26 -0.0
1,391,162 - amiB BNILDI_06955 0.27 -0.1
1,391,162 - amiB BNILDI_06955 0.27 -0.0
1,391,162 - amiB BNILDI_06955 0.27 -0.8
1,391,162 - amiB BNILDI_06955 0.27 +0.2
1,391,302 - amiB BNILDI_06955 0.37 +0.2
1,391,319 + amiB BNILDI_06955 0.38 +0.4
1,391,332 - amiB BNILDI_06955 0.39 -0.5

Or see this region's nucleotide sequence