Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sugE and blc overlap by 4 nucleotides blc and blaEC are separated by 88 nucleotides blaEC and frdD are separated by 62 nucleotides frdD and BNILDI_06875 are separated by 10 nucleotides BNILDI_06875 and frdB are separated by 10 nucleotides
BNILDI_06855: sugE - quaternary ammonium compound efflux SMR transporter SugE, at 1,372,172 to 1,372,489
sugE
BNILDI_06860: blc - lipocalin Blc, at 1,372,486 to 1,373,019
blc
BNILDI_06865: blaEC - BlaEC family class C beta-lactamase, at 1,373,108 to 1,374,241
blaEC
BNILDI_06870: frdD - fumarate reductase subunit FrdD, at 1,374,304 to 1,374,663
frdD
BNILDI_06875: BNILDI_06875 - Fumarate reductase subunit C, at 1,374,674 to 1,375,069
_06875
BNILDI_06880: frdB - fumarate reductase iron-sulfur protein, at 1,375,080 to 1,375,814
frdB
Position (kb)
1373
1374
1375 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1372.171 kb on + strand at 1372.171 kb on + strand at 1372.183 kb on + strand at 1372.184 kb on - strand at 1372.188 kb on + strand at 1372.188 kb on + strand at 1372.192 kb on + strand at 1372.235 kb on + strand, within sugE at 1372.270 kb on + strand, within sugE at 1372.271 kb on - strand, within sugE at 1372.346 kb on + strand, within sugE at 1372.452 kb on + strand, within sugE at 1372.453 kb on - strand, within sugE at 1372.463 kb on - strand at 1372.512 kb on + strand at 1372.512 kb on + strand at 1372.674 kb on - strand, within blc at 1372.683 kb on - strand, within blc at 1372.784 kb on + strand, within blc at 1372.785 kb on - strand, within blc at 1372.785 kb on - strand, within blc at 1372.791 kb on + strand, within blc at 1372.829 kb on + strand, within blc at 1372.829 kb on + strand, within blc at 1372.829 kb on + strand, within blc at 1372.931 kb on - strand, within blc at 1373.020 kb on - strand at 1373.051 kb on + strand at 1373.052 kb on - strand at 1373.186 kb on + strand at 1373.187 kb on - strand at 1373.188 kb on + strand at 1373.188 kb on + strand at 1373.221 kb on - strand at 1373.221 kb on - strand at 1373.221 kb on - strand at 1373.255 kb on + strand, within blaEC at 1373.256 kb on - strand, within blaEC at 1373.301 kb on - strand, within blaEC at 1373.397 kb on + strand, within blaEC at 1373.599 kb on - strand, within blaEC at 1373.621 kb on + strand, within blaEC at 1373.622 kb on - strand, within blaEC at 1373.635 kb on + strand, within blaEC at 1373.635 kb on + strand, within blaEC at 1373.635 kb on + strand, within blaEC at 1373.636 kb on - strand, within blaEC at 1373.636 kb on - strand, within blaEC at 1373.636 kb on - strand, within blaEC at 1373.745 kb on + strand, within blaEC at 1373.746 kb on - strand, within blaEC at 1373.873 kb on + strand, within blaEC at 1373.873 kb on + strand, within blaEC at 1373.874 kb on - strand, within blaEC at 1373.874 kb on - strand, within blaEC at 1373.874 kb on - strand, within blaEC at 1373.874 kb on - strand, within blaEC at 1373.876 kb on + strand, within blaEC at 1373.877 kb on - strand, within blaEC at 1373.940 kb on - strand, within blaEC at 1374.075 kb on + strand, within blaEC at 1374.075 kb on + strand, within blaEC at 1374.076 kb on - strand, within blaEC at 1374.076 kb on - strand, within blaEC at 1374.079 kb on - strand, within blaEC at 1374.134 kb on + strand at 1374.135 kb on - strand at 1374.145 kb on + strand at 1374.146 kb on - strand at 1374.164 kb on - strand at 1374.209 kb on - strand at 1374.212 kb on + strand at 1374.212 kb on + strand at 1374.212 kb on + strand at 1374.241 kb on + strand at 1374.241 kb on + strand at 1374.241 kb on + strand at 1374.242 kb on - strand at 1374.242 kb on - strand at 1374.242 kb on - strand at 1374.242 kb on - strand at 1374.257 kb on + strand at 1374.300 kb on + strand at 1374.313 kb on - strand at 1374.417 kb on + strand, within frdD at 1374.424 kb on + strand, within frdD at 1374.424 kb on + strand, within frdD at 1374.425 kb on - strand, within frdD at 1374.598 kb on - strand, within frdD at 1374.598 kb on - strand, within frdD at 1374.625 kb on + strand, within frdD at 1374.657 kb on + strand at 1374.658 kb on - strand at 1374.682 kb on - strand at 1374.701 kb on + strand at 1374.774 kb on - strand, within BNILDI_06875 at 1374.994 kb on - strand, within BNILDI_06875 at 1375.047 kb on + strand at 1375.047 kb on + strand at 1375.048 kb on - strand at 1375.048 kb on - strand at 1375.061 kb on - strand at 1375.126 kb on - strand at 1375.209 kb on + strand, within frdB at 1375.209 kb on + strand, within frdB at 1375.238 kb on + strand, within frdB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,372,171 + +0.1 1,372,171 + -3.2 1,372,183 + -0.3 1,372,184 - +0.3 1,372,188 + -0.3 1,372,188 + +0.1 1,372,192 + -0.5 1,372,235 + sugE BNILDI_06855 0.20 -0.3 1,372,270 + sugE BNILDI_06855 0.31 -1.0 1,372,271 - sugE BNILDI_06855 0.31 -0.5 1,372,346 + sugE BNILDI_06855 0.55 -0.2 1,372,452 + sugE BNILDI_06855 0.88 -1.3 1,372,453 - sugE BNILDI_06855 0.88 +0.0 1,372,463 - -0.5 1,372,512 + -0.3 1,372,512 + -0.1 1,372,674 - blc BNILDI_06860 0.35 +0.1 1,372,683 - blc BNILDI_06860 0.37 +0.4 1,372,784 + blc BNILDI_06860 0.56 +0.1 1,372,785 - blc BNILDI_06860 0.56 -0.6 1,372,785 - blc BNILDI_06860 0.56 +0.1 1,372,791 + blc BNILDI_06860 0.57 +0.5 1,372,829 + blc BNILDI_06860 0.64 -0.2 1,372,829 + blc BNILDI_06860 0.64 +0.3 1,372,829 + blc BNILDI_06860 0.64 +0.7 1,372,931 - blc BNILDI_06860 0.83 +0.1 1,373,020 - -0.1 1,373,051 + +0.8 1,373,052 - -0.1 1,373,186 + -0.6 1,373,187 - -0.0 1,373,188 + -1.0 1,373,188 + +0.2 1,373,221 - -0.1 1,373,221 - -1.5 1,373,221 - -0.8 1,373,255 + blaEC BNILDI_06865 0.13 -0.4 1,373,256 - blaEC BNILDI_06865 0.13 -1.2 1,373,301 - blaEC BNILDI_06865 0.17 +0.7 1,373,397 + blaEC BNILDI_06865 0.25 -0.3 1,373,599 - blaEC BNILDI_06865 0.43 +1.5 1,373,621 + blaEC BNILDI_06865 0.45 +1.1 1,373,622 - blaEC BNILDI_06865 0.45 +0.0 1,373,635 + blaEC BNILDI_06865 0.46 -3.3 1,373,635 + blaEC BNILDI_06865 0.46 +0.7 1,373,635 + blaEC BNILDI_06865 0.46 -0.4 1,373,636 - blaEC BNILDI_06865 0.47 -0.2 1,373,636 - blaEC BNILDI_06865 0.47 +0.2 1,373,636 - blaEC BNILDI_06865 0.47 -1.3 1,373,745 + blaEC BNILDI_06865 0.56 +0.2 1,373,746 - blaEC BNILDI_06865 0.56 -0.4 1,373,873 + blaEC BNILDI_06865 0.67 -0.7 1,373,873 + blaEC BNILDI_06865 0.67 -0.3 1,373,874 - blaEC BNILDI_06865 0.68 -0.4 1,373,874 - blaEC BNILDI_06865 0.68 +0.2 1,373,874 - blaEC BNILDI_06865 0.68 -0.1 1,373,874 - blaEC BNILDI_06865 0.68 -1.6 1,373,876 + blaEC BNILDI_06865 0.68 -0.0 1,373,877 - blaEC BNILDI_06865 0.68 +0.1 1,373,940 - blaEC BNILDI_06865 0.73 -0.1 1,374,075 + blaEC BNILDI_06865 0.85 -0.8 1,374,075 + blaEC BNILDI_06865 0.85 -0.3 1,374,076 - blaEC BNILDI_06865 0.85 -0.8 1,374,076 - blaEC BNILDI_06865 0.85 -0.2 1,374,079 - blaEC BNILDI_06865 0.86 +0.0 1,374,134 + +0.0 1,374,135 - -1.3 1,374,145 + +0.0 1,374,146 - -0.1 1,374,164 - +0.6 1,374,209 - -2.4 1,374,212 + +0.2 1,374,212 + -0.0 1,374,212 + +0.3 1,374,241 + -2.9 1,374,241 + +0.1 1,374,241 + +0.7 1,374,242 - +0.2 1,374,242 - -4.8 1,374,242 - +0.1 1,374,242 - -0.5 1,374,257 + +0.2 1,374,300 + -1.4 1,374,313 - +0.3 1,374,417 + frdD BNILDI_06870 0.31 -2.5 1,374,424 + frdD BNILDI_06870 0.33 -1.3 1,374,424 + frdD BNILDI_06870 0.33 -1.2 1,374,425 - frdD BNILDI_06870 0.34 -0.2 1,374,598 - frdD BNILDI_06870 0.82 +0.9 1,374,598 - frdD BNILDI_06870 0.82 -1.2 1,374,625 + frdD BNILDI_06870 0.89 -0.2 1,374,657 + +0.6 1,374,658 - +0.3 1,374,682 - +0.6 1,374,701 + +0.6 1,374,774 - BNILDI_06875 0.25 +1.7 1,374,994 - BNILDI_06875 0.81 +0.3 1,375,047 + -0.3 1,375,047 + +0.7 1,375,048 - +1.1 1,375,048 - -0.7 1,375,061 - -0.2 1,375,126 - +0.1 1,375,209 + frdB BNILDI_06880 0.18 -2.7 1,375,209 + frdB BNILDI_06880 0.18 +2.0 1,375,238 + frdB BNILDI_06880 0.21 +1.2
Or see this region's nucleotide sequence