Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiC and rsd are separated by 232 nucleotides rsd and nudC are separated by 94 nucleotides nudC and hemE are separated by 39 nucleotides
BNILDI_06005: thiC - phosphomethylpyrimidine synthase ThiC, at 1,193,839 to 1,195,734
thiC
BNILDI_06010: rsd - sigma D regulator, at 1,195,967 to 1,196,443
rsd
BNILDI_06015: nudC - NAD(+) diphosphatase, at 1,196,538 to 1,197,311
nudC
BNILDI_06020: hemE - uroporphyrinogen decarboxylase, at 1,197,351 to 1,198,415
hemE
Position (kb)
1195
1196
1197 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1195.081 kb on - strand, within thiC at 1195.192 kb on + strand, within thiC at 1195.328 kb on - strand, within thiC at 1195.330 kb on + strand, within thiC at 1195.330 kb on + strand, within thiC at 1195.330 kb on + strand, within thiC at 1195.331 kb on - strand, within thiC at 1195.331 kb on - strand, within thiC at 1195.331 kb on - strand, within thiC at 1195.331 kb on - strand, within thiC at 1195.632 kb on + strand at 1195.634 kb on + strand at 1195.634 kb on + strand at 1195.635 kb on - strand at 1195.681 kb on - strand at 1195.734 kb on + strand at 1195.735 kb on - strand at 1195.735 kb on - strand at 1195.735 kb on - strand at 1195.822 kb on + strand at 1195.823 kb on - strand at 1195.823 kb on - strand at 1195.844 kb on + strand at 1195.844 kb on + strand at 1195.895 kb on - strand at 1196.209 kb on + strand, within rsd at 1196.226 kb on + strand, within rsd at 1196.253 kb on + strand, within rsd at 1196.253 kb on + strand, within rsd at 1196.254 kb on - strand, within rsd at 1196.254 kb on - strand, within rsd at 1196.307 kb on + strand, within rsd at 1196.308 kb on - strand, within rsd at 1196.346 kb on + strand, within rsd at 1196.347 kb on - strand, within rsd at 1196.365 kb on + strand, within rsd at 1196.365 kb on + strand, within rsd at 1196.365 kb on + strand, within rsd at 1196.369 kb on + strand, within rsd at 1196.369 kb on + strand, within rsd at 1196.369 kb on + strand, within rsd at 1196.369 kb on + strand, within rsd at 1196.370 kb on - strand, within rsd at 1196.370 kb on - strand, within rsd at 1196.370 kb on - strand, within rsd at 1196.370 kb on - strand, within rsd at 1196.375 kb on + strand, within rsd at 1196.398 kb on + strand at 1196.399 kb on - strand at 1196.526 kb on - strand at 1196.537 kb on + strand at 1196.546 kb on + strand at 1196.549 kb on - strand at 1196.599 kb on + strand at 1196.707 kb on + strand, within nudC at 1196.707 kb on + strand, within nudC at 1196.708 kb on - strand, within nudC at 1196.826 kb on + strand, within nudC at 1196.827 kb on - strand, within nudC at 1196.827 kb on - strand, within nudC at 1196.827 kb on - strand, within nudC at 1196.853 kb on + strand, within nudC at 1196.854 kb on - strand, within nudC at 1196.854 kb on - strand, within nudC at 1196.930 kb on + strand, within nudC at 1196.930 kb on + strand, within nudC at 1196.931 kb on - strand, within nudC at 1196.931 kb on - strand, within nudC at 1196.999 kb on + strand, within nudC at 1197.004 kb on + strand, within nudC at 1197.004 kb on + strand, within nudC at 1197.005 kb on - strand, within nudC at 1197.149 kb on + strand, within nudC at 1197.271 kb on + strand at 1197.278 kb on + strand at 1197.278 kb on + strand at 1197.303 kb on + strand at 1197.315 kb on + strand at 1197.315 kb on + strand at 1197.316 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,195,081 - thiC BNILDI_06005 0.66 -0.2 1,195,192 + thiC BNILDI_06005 0.71 +1.5 1,195,328 - thiC BNILDI_06005 0.79 +0.7 1,195,330 + thiC BNILDI_06005 0.79 -0.4 1,195,330 + thiC BNILDI_06005 0.79 -2.0 1,195,330 + thiC BNILDI_06005 0.79 -0.7 1,195,331 - thiC BNILDI_06005 0.79 -0.5 1,195,331 - thiC BNILDI_06005 0.79 -3.4 1,195,331 - thiC BNILDI_06005 0.79 +0.4 1,195,331 - thiC BNILDI_06005 0.79 -0.1 1,195,632 + -0.5 1,195,634 + -2.9 1,195,634 + -0.8 1,195,635 - +0.1 1,195,681 - -0.4 1,195,734 + -1.6 1,195,735 - -0.5 1,195,735 - -0.4 1,195,735 - -0.0 1,195,822 + -0.5 1,195,823 - -0.3 1,195,823 - +0.1 1,195,844 + -0.1 1,195,844 + -0.3 1,195,895 - -0.5 1,196,209 + rsd BNILDI_06010 0.51 +1.8 1,196,226 + rsd BNILDI_06010 0.54 -0.1 1,196,253 + rsd BNILDI_06010 0.60 +0.1 1,196,253 + rsd BNILDI_06010 0.60 +1.8 1,196,254 - rsd BNILDI_06010 0.60 +0.8 1,196,254 - rsd BNILDI_06010 0.60 +0.3 1,196,307 + rsd BNILDI_06010 0.71 -0.1 1,196,308 - rsd BNILDI_06010 0.71 +1.1 1,196,346 + rsd BNILDI_06010 0.79 +0.1 1,196,347 - rsd BNILDI_06010 0.80 -1.4 1,196,365 + rsd BNILDI_06010 0.83 +0.2 1,196,365 + rsd BNILDI_06010 0.83 -2.2 1,196,365 + rsd BNILDI_06010 0.83 +0.7 1,196,369 + rsd BNILDI_06010 0.84 +0.5 1,196,369 + rsd BNILDI_06010 0.84 -1.4 1,196,369 + rsd BNILDI_06010 0.84 -0.5 1,196,369 + rsd BNILDI_06010 0.84 +0.5 1,196,370 - rsd BNILDI_06010 0.84 +0.6 1,196,370 - rsd BNILDI_06010 0.84 -1.1 1,196,370 - rsd BNILDI_06010 0.84 +0.9 1,196,370 - rsd BNILDI_06010 0.84 +0.6 1,196,375 + rsd BNILDI_06010 0.86 +0.2 1,196,398 + -0.1 1,196,399 - +0.3 1,196,526 - +0.1 1,196,537 + +1.4 1,196,546 + -0.2 1,196,549 - -0.7 1,196,599 + -0.6 1,196,707 + nudC BNILDI_06015 0.22 +1.2 1,196,707 + nudC BNILDI_06015 0.22 -0.9 1,196,708 - nudC BNILDI_06015 0.22 +0.4 1,196,826 + nudC BNILDI_06015 0.37 -0.3 1,196,827 - nudC BNILDI_06015 0.37 -1.0 1,196,827 - nudC BNILDI_06015 0.37 -0.6 1,196,827 - nudC BNILDI_06015 0.37 +2.1 1,196,853 + nudC BNILDI_06015 0.41 -2.5 1,196,854 - nudC BNILDI_06015 0.41 +1.5 1,196,854 - nudC BNILDI_06015 0.41 -0.0 1,196,930 + nudC BNILDI_06015 0.51 -1.8 1,196,930 + nudC BNILDI_06015 0.51 -0.8 1,196,931 - nudC BNILDI_06015 0.51 +0.3 1,196,931 - nudC BNILDI_06015 0.51 +3.2 1,196,999 + nudC BNILDI_06015 0.60 -1.7 1,197,004 + nudC BNILDI_06015 0.60 -1.7 1,197,004 + nudC BNILDI_06015 0.60 -0.5 1,197,005 - nudC BNILDI_06015 0.60 +0.0 1,197,149 + nudC BNILDI_06015 0.79 +0.3 1,197,271 + +1.2 1,197,278 + +0.7 1,197,278 + +0.1 1,197,303 + +0.8 1,197,315 + -0.1 1,197,315 + +0.3 1,197,316 - -0.5
Or see this region's nucleotide sequence