Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yifL and dapF are separated by 36 nucleotides dapF and yigA overlap by 4 nucleotides yigA and xerC overlap by 4 nucleotides xerC and yigB overlap by 1 nucleotides
BNILDI_04970: yifL - Uncharacterized lipoprotein YifL, at 984,885 to 985,088
yifL
BNILDI_04975: dapF - diaminopimelate epimerase, at 985,125 to 985,949
dapF
BNILDI_04980: yigA - Uncharacterized protein YigA, at 985,946 to 986,653
yigA
BNILDI_04985: xerC - tyrosine recombinase XerC, at 986,650 to 987,546
xerC
BNILDI_04990: yigB - 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YigB, at 987,546 to 988,262
yigB
Position (kb) 985
986
987 Strain fitness (log2 ratio) -2
-1
0
1 at 984.964 kb on - strand, within yifL at 984.964 kb on - strand, within yifL at 984.980 kb on + strand, within yifL at 985.083 kb on + strand at 985.083 kb on + strand at 985.945 kb on + strand at 985.945 kb on + strand at 985.945 kb on + strand at 986.131 kb on + strand, within yigA at 986.138 kb on - strand, within yigA at 986.138 kb on - strand, within yigA at 986.211 kb on + strand, within yigA at 986.212 kb on - strand, within yigA at 986.216 kb on + strand, within yigA at 986.257 kb on + strand, within yigA at 986.257 kb on + strand, within yigA at 986.430 kb on - strand, within yigA at 986.469 kb on + strand, within yigA at 986.470 kb on - strand, within yigA at 986.546 kb on - strand, within yigA at 986.592 kb on + strand at 986.672 kb on + strand at 986.684 kb on + strand at 986.684 kb on + strand at 986.684 kb on + strand at 987.003 kb on + strand, within xerC at 987.396 kb on + strand, within xerC at 987.509 kb on - strand at 987.609 kb on + strand at 987.609 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 984,964 - yifL BNILDI_04970 0.39 -1.9 984,964 - yifL BNILDI_04970 0.39 -1.5 984,980 + yifL BNILDI_04970 0.47 +1.4 985,083 + +0.8 985,083 + -0.8 985,945 + +0.9 985,945 + -1.2 985,945 + +0.2 986,131 + yigA BNILDI_04980 0.26 -0.2 986,138 - yigA BNILDI_04980 0.27 -0.6 986,138 - yigA BNILDI_04980 0.27 -0.8 986,211 + yigA BNILDI_04980 0.37 -0.6 986,212 - yigA BNILDI_04980 0.38 +0.4 986,216 + yigA BNILDI_04980 0.38 +1.0 986,257 + yigA BNILDI_04980 0.44 -2.2 986,257 + yigA BNILDI_04980 0.44 +0.2 986,430 - yigA BNILDI_04980 0.68 -1.3 986,469 + yigA BNILDI_04980 0.74 -0.5 986,470 - yigA BNILDI_04980 0.74 -1.0 986,546 - yigA BNILDI_04980 0.85 +0.2 986,592 + +0.1 986,672 + +1.4 986,684 + -0.4 986,684 + -1.1 986,684 + -1.9 987,003 + xerC BNILDI_04985 0.39 -1.2 987,396 + xerC BNILDI_04985 0.83 -1.0 987,509 - +1.6 987,609 + +1.3 987,609 + +0.2
Or see this region's nucleotide sequence