Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mnmG and mioC are separated by 378 nucleotides mioC and asnC are separated by 89 nucleotides asnC and asnA are separated by 151 nucleotides
BNILDI_04605: mnmG - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG, at 910,722 to 912,611
mnmG
BNILDI_04610: mioC - FMN-binding protein MioC, at 912,990 to 913,433
mioC
BNILDI_04615: asnC - transcriptional regulator AsnC, at 913,523 to 913,981
asnC
BNILDI_04620: asnA - aspartate--ammonia ligase, at 914,133 to 915,125
asnA
Position (kb) 912
913
914 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 912.103 kb on + strand, within mnmG at 912.228 kb on - strand, within mnmG at 912.228 kb on - strand, within mnmG at 912.285 kb on - strand, within mnmG at 912.670 kb on - strand at 912.698 kb on + strand at 912.699 kb on - strand at 912.699 kb on - strand at 912.714 kb on - strand at 912.727 kb on - strand at 912.987 kb on + strand at 912.991 kb on + strand at 912.991 kb on + strand at 913.124 kb on + strand, within mioC at 913.150 kb on + strand, within mioC at 913.150 kb on + strand, within mioC at 913.225 kb on + strand, within mioC at 913.226 kb on - strand, within mioC at 913.226 kb on - strand, within mioC at 913.226 kb on - strand, within mioC at 913.250 kb on + strand, within mioC at 913.302 kb on + strand, within mioC at 913.302 kb on + strand, within mioC at 913.340 kb on + strand, within mioC at 913.341 kb on - strand, within mioC at 913.385 kb on - strand, within mioC at 913.415 kb on + strand at 913.415 kb on + strand at 913.416 kb on - strand at 913.425 kb on - strand at 913.447 kb on + strand at 913.451 kb on - strand at 913.466 kb on + strand at 913.467 kb on - strand at 913.504 kb on - strand at 913.613 kb on - strand, within asnC at 913.613 kb on - strand, within asnC at 913.616 kb on + strand, within asnC at 913.617 kb on - strand, within asnC at 913.617 kb on - strand, within asnC at 913.617 kb on - strand, within asnC at 913.617 kb on - strand, within asnC at 913.617 kb on - strand, within asnC at 913.655 kb on + strand, within asnC at 913.669 kb on - strand, within asnC at 913.683 kb on + strand, within asnC at 913.683 kb on + strand, within asnC at 913.684 kb on - strand, within asnC at 913.737 kb on + strand, within asnC at 913.757 kb on + strand, within asnC at 913.757 kb on + strand, within asnC at 913.758 kb on - strand, within asnC at 913.759 kb on + strand, within asnC at 913.760 kb on - strand, within asnC at 913.778 kb on + strand, within asnC at 913.779 kb on - strand, within asnC at 913.779 kb on - strand, within asnC at 913.779 kb on - strand, within asnC at 913.813 kb on + strand, within asnC at 913.972 kb on - strand at 913.982 kb on - strand at 914.145 kb on + strand at 914.145 kb on + strand at 914.145 kb on + strand at 914.168 kb on + strand at 914.270 kb on + strand, within asnA at 914.270 kb on + strand, within asnA at 914.271 kb on - strand, within asnA at 914.303 kb on - strand, within asnA at 914.409 kb on + strand, within asnA at 914.409 kb on + strand, within asnA at 914.410 kb on - strand, within asnA
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 912,103 + mnmG BNILDI_04605 0.73 -0.6 912,228 - mnmG BNILDI_04605 0.80 -2.0 912,228 - mnmG BNILDI_04605 0.80 -0.2 912,285 - mnmG BNILDI_04605 0.83 +1.0 912,670 - +0.6 912,698 + +1.8 912,699 - +0.1 912,699 - +0.1 912,714 - -1.6 912,727 - -0.2 912,987 + -0.9 912,991 + -2.1 912,991 + -0.3 913,124 + mioC BNILDI_04610 0.30 +0.8 913,150 + mioC BNILDI_04610 0.36 +0.6 913,150 + mioC BNILDI_04610 0.36 -0.2 913,225 + mioC BNILDI_04610 0.53 +0.7 913,226 - mioC BNILDI_04610 0.53 -3.1 913,226 - mioC BNILDI_04610 0.53 +0.1 913,226 - mioC BNILDI_04610 0.53 +0.1 913,250 + mioC BNILDI_04610 0.59 +0.5 913,302 + mioC BNILDI_04610 0.70 +0.8 913,302 + mioC BNILDI_04610 0.70 +0.3 913,340 + mioC BNILDI_04610 0.79 -0.7 913,341 - mioC BNILDI_04610 0.79 -0.6 913,385 - mioC BNILDI_04610 0.89 +0.1 913,415 + +1.3 913,415 + -3.1 913,416 - -0.3 913,425 - +1.4 913,447 + -0.3 913,451 - +0.2 913,466 + +0.3 913,467 - -0.5 913,504 - -1.5 913,613 - asnC BNILDI_04615 0.20 +0.6 913,613 - asnC BNILDI_04615 0.20 +0.1 913,616 + asnC BNILDI_04615 0.20 +1.2 913,617 - asnC BNILDI_04615 0.20 +0.1 913,617 - asnC BNILDI_04615 0.20 -0.4 913,617 - asnC BNILDI_04615 0.20 +0.9 913,617 - asnC BNILDI_04615 0.20 +0.1 913,617 - asnC BNILDI_04615 0.20 +0.2 913,655 + asnC BNILDI_04615 0.29 +0.4 913,669 - asnC BNILDI_04615 0.32 -2.9 913,683 + asnC BNILDI_04615 0.35 +0.1 913,683 + asnC BNILDI_04615 0.35 -0.5 913,684 - asnC BNILDI_04615 0.35 +0.1 913,737 + asnC BNILDI_04615 0.47 -0.1 913,757 + asnC BNILDI_04615 0.51 -0.8 913,757 + asnC BNILDI_04615 0.51 +2.2 913,758 - asnC BNILDI_04615 0.51 +3.0 913,759 + asnC BNILDI_04615 0.51 +0.1 913,760 - asnC BNILDI_04615 0.52 +1.2 913,778 + asnC BNILDI_04615 0.56 -1.6 913,779 - asnC BNILDI_04615 0.56 +0.2 913,779 - asnC BNILDI_04615 0.56 -1.3 913,779 - asnC BNILDI_04615 0.56 +0.2 913,813 + asnC BNILDI_04615 0.63 +0.2 913,972 - +0.3 913,982 - +0.1 914,145 + +0.3 914,145 + -1.3 914,145 + +0.3 914,168 + +1.0 914,270 + asnA BNILDI_04620 0.14 +0.6 914,270 + asnA BNILDI_04620 0.14 +0.9 914,271 - asnA BNILDI_04620 0.14 +0.4 914,303 - asnA BNILDI_04620 0.17 +0.6 914,409 + asnA BNILDI_04620 0.28 +2.1 914,409 + asnA BNILDI_04620 0.28 -0.0 914,410 - asnA BNILDI_04620 0.28 -0.2
Or see this region's nucleotide sequence