Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmL and cysE are separated by 52 nucleotides cysE and gpsA are separated by 79 nucleotides gpsA and secB overlap by 1 nucleotides secB and grxC are separated by 62 nucleotides grxC and yibN are separated by 141 nucleotides
BNILDI_03870: trmL - tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine (34)-2'-O)-methyltransferase TrmL, at 766,063 to 766,536
trmL
BNILDI_03875: cysE - serine O-acetyltransferase, at 766,589 to 767,410
cysE
BNILDI_03880: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 767,490 to 768,509
gpsA
BNILDI_03885: secB - protein-export chaperone SecB, at 768,509 to 768,976
secB
BNILDI_03890: grxC - glutaredoxin 3, at 769,039 to 769,290
grxC
BNILDI_03895: yibN - Uncharacterized protein YibN, at 769,432 to 769,863
yibN
Position (kb) 767
768
769 Strain fitness (log2 ratio) -2
-1
0
1 at 766.535 kb on - strand at 766.543 kb on + strand at 766.560 kb on + strand at 766.560 kb on + strand at 766.561 kb on - strand at 766.616 kb on - strand at 766.616 kb on - strand at 766.616 kb on - strand at 766.622 kb on - strand at 767.433 kb on - strand at 768.992 kb on - strand at 769.009 kb on - strand at 769.021 kb on - strand at 769.041 kb on - strand at 769.083 kb on - strand, within grxC at 769.083 kb on - strand, within grxC at 769.272 kb on - strand at 769.296 kb on - strand at 769.296 kb on - strand at 769.367 kb on + strand at 769.373 kb on + strand at 769.428 kb on + strand at 769.433 kb on + strand at 769.498 kb on + strand, within yibN at 769.498 kb on + strand, within yibN at 769.499 kb on - strand, within yibN at 769.499 kb on - strand, within yibN at 769.499 kb on - strand, within yibN
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 766,535 - -0.4 766,543 + -0.2 766,560 + +0.4 766,560 + +1.3 766,561 - -0.2 766,616 - -0.7 766,616 - -0.9 766,616 - -1.1 766,622 - +0.6 767,433 - +0.3 768,992 - -1.0 769,009 - -0.3 769,021 - -1.0 769,041 - -0.6 769,083 - grxC BNILDI_03890 0.17 +0.8 769,083 - grxC BNILDI_03890 0.17 -0.3 769,272 - +0.6 769,296 - -1.7 769,296 - +0.1 769,367 + +1.2 769,373 + -0.4 769,428 + -0.8 769,433 + +0.0 769,498 + yibN BNILDI_03895 0.15 -2.7 769,498 + yibN BNILDI_03895 0.15 +0.5 769,499 - yibN BNILDI_03895 0.16 -0.6 769,499 - yibN BNILDI_03895 0.16 -1.6 769,499 - yibN BNILDI_03895 0.16 +0.2
Or see this region's nucleotide sequence