Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhjY and tag are separated by 157 nucleotides tag and yiaC overlap by 4 nucleotides yiaC and bisC overlap by 32 nucleotides
BNILDI_03555: yhjY - Uncharacterized protein YhjY, at 694,357 to 695,055
yhjY
BNILDI_03560: tag - DNA-3-methyladenine glycosylase I, at 695,213 to 695,776
tag
BNILDI_03565: yiaC - N-acetyltransferase, at 695,773 to 696,213
yiaC
BNILDI_03570: bisC - biotin sulfoxide reductase, at 696,182 to 698,515
bisC
Position (kb) 695
696
697 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 694.860 kb on + strand, within yhjY at 694.876 kb on - strand, within yhjY at 694.876 kb on - strand, within yhjY at 694.914 kb on - strand, within yhjY at 694.951 kb on - strand, within yhjY at 694.952 kb on + strand, within yhjY at 694.952 kb on + strand, within yhjY at 694.953 kb on - strand, within yhjY at 694.953 kb on - strand, within yhjY at 694.953 kb on - strand, within yhjY at 695.021 kb on - strand at 695.021 kb on - strand at 695.090 kb on + strand at 695.090 kb on + strand at 695.091 kb on - strand at 695.094 kb on - strand at 695.122 kb on + strand at 695.123 kb on - strand at 695.125 kb on + strand at 695.200 kb on - strand at 695.212 kb on + strand at 695.213 kb on - strand at 695.249 kb on + strand at 695.251 kb on + strand at 695.251 kb on + strand at 695.252 kb on - strand at 695.258 kb on + strand at 695.266 kb on + strand at 695.497 kb on + strand, within tag at 695.498 kb on - strand, within tag at 695.498 kb on - strand, within tag at 695.571 kb on + strand, within tag at 695.833 kb on - strand, within yiaC at 695.855 kb on + strand, within yiaC at 695.856 kb on - strand, within yiaC at 695.856 kb on - strand, within yiaC at 695.902 kb on + strand, within yiaC at 695.902 kb on + strand, within yiaC at 695.902 kb on + strand, within yiaC at 695.903 kb on - strand, within yiaC at 695.968 kb on - strand, within yiaC at 696.046 kb on + strand, within yiaC at 696.046 kb on + strand, within yiaC at 696.046 kb on + strand, within yiaC at 696.046 kb on + strand, within yiaC at 696.047 kb on - strand, within yiaC at 696.184 kb on - strand at 696.184 kb on - strand at 696.211 kb on + strand at 696.234 kb on + strand at 696.235 kb on - strand at 696.259 kb on + strand at 696.400 kb on + strand at 696.401 kb on - strand at 696.476 kb on + strand, within bisC at 696.602 kb on + strand, within bisC at 696.782 kb on - strand, within bisC at 696.782 kb on - strand, within bisC at 696.897 kb on + strand, within bisC at 696.898 kb on - strand, within bisC at 697.012 kb on - strand, within bisC at 697.059 kb on + strand, within bisC at 697.059 kb on + strand, within bisC at 697.060 kb on - strand, within bisC
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 694,860 + yhjY BNILDI_03555 0.72 +0.2 694,876 - yhjY BNILDI_03555 0.74 -0.7 694,876 - yhjY BNILDI_03555 0.74 -0.4 694,914 - yhjY BNILDI_03555 0.80 +0.6 694,951 - yhjY BNILDI_03555 0.85 +0.0 694,952 + yhjY BNILDI_03555 0.85 -0.6 694,952 + yhjY BNILDI_03555 0.85 -0.7 694,953 - yhjY BNILDI_03555 0.85 -0.9 694,953 - yhjY BNILDI_03555 0.85 -0.2 694,953 - yhjY BNILDI_03555 0.85 -0.8 695,021 - +0.1 695,021 - +0.2 695,090 + +0.5 695,090 + -0.2 695,091 - -3.4 695,094 - -0.0 695,122 + -0.1 695,123 - +0.5 695,125 + -0.1 695,200 - +0.8 695,212 + -1.5 695,213 - +0.1 695,249 + +0.0 695,251 + -1.3 695,251 + -1.2 695,252 - -2.2 695,258 + +0.5 695,266 + +0.5 695,497 + tag BNILDI_03560 0.50 +0.1 695,498 - tag BNILDI_03560 0.51 +0.6 695,498 - tag BNILDI_03560 0.51 -0.1 695,571 + tag BNILDI_03560 0.63 +0.7 695,833 - yiaC BNILDI_03565 0.14 +0.6 695,855 + yiaC BNILDI_03565 0.19 +0.1 695,856 - yiaC BNILDI_03565 0.19 +1.1 695,856 - yiaC BNILDI_03565 0.19 +0.3 695,902 + yiaC BNILDI_03565 0.29 +0.7 695,902 + yiaC BNILDI_03565 0.29 -0.1 695,902 + yiaC BNILDI_03565 0.29 -1.0 695,903 - yiaC BNILDI_03565 0.29 +0.6 695,968 - yiaC BNILDI_03565 0.44 +0.7 696,046 + yiaC BNILDI_03565 0.62 -0.2 696,046 + yiaC BNILDI_03565 0.62 +0.6 696,046 + yiaC BNILDI_03565 0.62 -0.1 696,046 + yiaC BNILDI_03565 0.62 -1.1 696,047 - yiaC BNILDI_03565 0.62 -0.4 696,184 - +0.1 696,184 - -0.4 696,211 + +0.8 696,234 + +0.9 696,235 - -2.9 696,259 + -0.6 696,400 + -0.2 696,401 - +0.2 696,476 + bisC BNILDI_03570 0.13 -0.4 696,602 + bisC BNILDI_03570 0.18 +0.3 696,782 - bisC BNILDI_03570 0.26 -0.0 696,782 - bisC BNILDI_03570 0.26 -0.0 696,897 + bisC BNILDI_03570 0.31 +0.6 696,898 - bisC BNILDI_03570 0.31 -0.8 697,012 - bisC BNILDI_03570 0.36 -0.1 697,059 + bisC BNILDI_03570 0.38 +0.9 697,059 + bisC BNILDI_03570 0.38 +0.2 697,060 - bisC BNILDI_03570 0.38 -0.2
Or see this region's nucleotide sequence