Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hdeB and hdeA are separated by 103 nucleotides hdeA and hdeD are separated by 254 nucleotides hdeD and gadE are separated by 798 nucleotides
BNILDI_03280: hdeB - acid-activated periplasmic chaperone HdeB, at 635,619 to 635,957
hdeB
BNILDI_03285: hdeA - acid-activated periplasmic chaperone HdeA, at 636,061 to 636,393
hdeA
BNILDI_03290: hdeD - acid-resistance protein HdeD, at 636,648 to 637,220
hdeD
BNILDI_03295: gadE - acid resistance transcriptional activator GadE, at 638,019 to 638,546
gadE
Position (kb) 636
637
638 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 635.753 kb on + strand, within hdeB at 635.754 kb on - strand, within hdeB at 635.756 kb on - strand, within hdeB at 635.757 kb on + strand, within hdeB at 635.820 kb on - strand, within hdeB at 635.972 kb on - strand at 636.132 kb on + strand, within hdeA at 636.147 kb on + strand, within hdeA at 636.182 kb on - strand, within hdeA at 636.182 kb on - strand, within hdeA at 636.396 kb on + strand at 636.397 kb on - strand at 636.454 kb on - strand at 636.628 kb on + strand at 636.648 kb on - strand at 636.652 kb on + strand at 636.652 kb on + strand at 636.658 kb on + strand at 636.659 kb on - strand at 636.912 kb on + strand, within hdeD at 636.928 kb on - strand, within hdeD at 636.978 kb on - strand, within hdeD at 637.166 kb on - strand at 637.166 kb on - strand at 637.166 kb on - strand at 637.218 kb on + strand at 637.218 kb on + strand at 637.219 kb on - strand at 637.219 kb on - strand at 637.274 kb on - strand at 637.300 kb on + strand at 637.332 kb on - strand at 637.512 kb on - strand at 637.559 kb on + strand at 637.695 kb on + strand at 637.695 kb on + strand at 637.760 kb on + strand at 637.761 kb on - strand at 637.833 kb on - strand at 637.833 kb on - strand at 637.846 kb on - strand at 637.865 kb on + strand at 637.876 kb on + strand at 637.877 kb on - strand at 637.889 kb on + strand at 637.890 kb on - strand at 637.902 kb on - strand at 637.966 kb on + strand at 637.967 kb on - strand at 637.967 kb on - strand at 638.079 kb on - strand, within gadE at 638.160 kb on + strand, within gadE
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 635,753 + hdeB BNILDI_03280 0.40 +0.3 635,754 - hdeB BNILDI_03280 0.40 +0.1 635,756 - hdeB BNILDI_03280 0.40 +1.1 635,757 + hdeB BNILDI_03280 0.41 -0.1 635,820 - hdeB BNILDI_03280 0.59 -0.3 635,972 - -0.1 636,132 + hdeA BNILDI_03285 0.21 -0.0 636,147 + hdeA BNILDI_03285 0.26 +0.1 636,182 - hdeA BNILDI_03285 0.36 +0.3 636,182 - hdeA BNILDI_03285 0.36 -0.5 636,396 + +0.6 636,397 - +0.5 636,454 - +0.8 636,628 + +0.0 636,648 - +0.4 636,652 + +0.1 636,652 + -0.0 636,658 + +1.2 636,659 - +0.9 636,912 + hdeD BNILDI_03290 0.46 +0.4 636,928 - hdeD BNILDI_03290 0.49 +0.2 636,978 - hdeD BNILDI_03290 0.58 +0.4 637,166 - -1.0 637,166 - -0.2 637,166 - -1.4 637,218 + +0.1 637,218 + +0.3 637,219 - -0.9 637,219 - +0.1 637,274 - +0.2 637,300 + -0.5 637,332 - -0.0 637,512 - +1.1 637,559 + -0.2 637,695 + -3.2 637,695 + +0.2 637,760 + +0.1 637,761 - +0.0 637,833 - +0.8 637,833 - +0.4 637,846 - +1.1 637,865 + +0.4 637,876 + +0.7 637,877 - -1.0 637,889 + +0.3 637,890 - +0.2 637,902 - +0.1 637,966 + -0.6 637,967 - -0.7 637,967 - -0.1 638,079 - gadE BNILDI_03295 0.11 +0.2 638,160 + gadE BNILDI_03295 0.27 +1.3
Or see this region's nucleotide sequence