Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpnA and bioH are separated by 28 nucleotides bioH and gntX are separated by 37 nucleotides gntX and BNILDI_02740 are separated by 58 nucleotides BNILDI_02740 and gntT are separated by 360 nucleotides
BNILDI_02725: rpnA - recombination-promoting nuclease RpnA, at 519,957 to 520,835
rpnA
BNILDI_02730: bioH - pimeloyl-ACP methyl ester esterase BioH, at 520,864 to 521,634
bioH
BNILDI_02735: gntX - DNA utilization protein GntX, at 521,672 to 522,355
gntX
BNILDI_02740: BNILDI_02740 - NfuA family Fe-S biogenesis protein, at 522,414 to 522,989
_02740
BNILDI_02745: gntT - gluconate transporter, at 523,350 to 524,666
gntT
Position (kb) 521
522
523 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 520.739 kb on + strand, within rpnA at 520.739 kb on + strand, within rpnA at 520.739 kb on + strand, within rpnA at 520.739 kb on + strand, within rpnA at 520.739 kb on + strand, within rpnA at 520.740 kb on - strand, within rpnA at 520.751 kb on - strand at 520.776 kb on - strand at 520.833 kb on + strand at 520.865 kb on + strand at 520.950 kb on + strand, within bioH at 520.950 kb on + strand, within bioH at 520.951 kb on - strand, within bioH at 520.951 kb on - strand, within bioH at 520.951 kb on - strand, within bioH at 520.951 kb on - strand, within bioH at 520.952 kb on + strand, within bioH at 520.953 kb on - strand, within bioH at 520.953 kb on - strand, within bioH at 520.953 kb on - strand, within bioH at 521.027 kb on + strand, within bioH at 521.027 kb on + strand, within bioH at 521.027 kb on + strand, within bioH at 521.027 kb on + strand, within bioH at 521.028 kb on - strand, within bioH at 521.028 kb on - strand, within bioH at 521.028 kb on - strand, within bioH at 521.106 kb on + strand, within bioH at 521.197 kb on + strand, within bioH at 521.302 kb on - strand, within bioH at 521.514 kb on + strand, within bioH at 521.515 kb on - strand, within bioH at 521.515 kb on - strand, within bioH at 521.527 kb on - strand, within bioH at 521.527 kb on - strand, within bioH at 521.628 kb on + strand at 521.628 kb on + strand at 521.657 kb on + strand at 521.671 kb on + strand at 521.671 kb on + strand at 521.671 kb on + strand at 521.671 kb on + strand at 521.671 kb on + strand at 521.671 kb on + strand at 521.671 kb on + strand at 521.671 kb on + strand at 521.672 kb on - strand at 521.672 kb on - strand at 521.672 kb on - strand at 521.672 kb on - strand at 521.676 kb on + strand at 521.677 kb on - strand at 521.683 kb on - strand at 521.691 kb on + strand at 521.692 kb on - strand at 521.770 kb on + strand, within gntX at 521.771 kb on - strand, within gntX at 521.778 kb on + strand, within gntX at 521.880 kb on - strand, within gntX at 521.965 kb on - strand, within gntX at 521.977 kb on - strand, within gntX at 522.121 kb on + strand, within gntX at 522.239 kb on - strand, within gntX at 522.410 kb on + strand at 522.410 kb on + strand at 522.541 kb on - strand, within BNILDI_02740 at 522.603 kb on + strand, within BNILDI_02740 at 522.616 kb on + strand, within BNILDI_02740 at 522.679 kb on + strand, within BNILDI_02740 at 522.680 kb on - strand, within BNILDI_02740 at 522.750 kb on + strand, within BNILDI_02740 at 522.750 kb on + strand, within BNILDI_02740 at 522.752 kb on + strand, within BNILDI_02740 at 522.981 kb on + strand at 522.981 kb on + strand at 522.984 kb on + strand at 522.984 kb on + strand at 522.987 kb on + strand at 522.988 kb on - strand at 522.988 kb on - strand at 522.988 kb on - strand at 522.988 kb on - strand at 523.117 kb on + strand at 523.118 kb on - strand at 523.118 kb on - strand at 523.118 kb on - strand at 523.235 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 520,739 + rpnA BNILDI_02725 0.89 -0.1 520,739 + rpnA BNILDI_02725 0.89 -0.5 520,739 + rpnA BNILDI_02725 0.89 +0.3 520,739 + rpnA BNILDI_02725 0.89 +0.4 520,739 + rpnA BNILDI_02725 0.89 +0.3 520,740 - rpnA BNILDI_02725 0.89 +0.2 520,751 - +0.9 520,776 - -1.3 520,833 + +0.9 520,865 + -0.1 520,950 + bioH BNILDI_02730 0.11 +0.1 520,950 + bioH BNILDI_02730 0.11 -0.2 520,951 - bioH BNILDI_02730 0.11 +0.3 520,951 - bioH BNILDI_02730 0.11 -1.5 520,951 - bioH BNILDI_02730 0.11 -0.5 520,951 - bioH BNILDI_02730 0.11 +0.3 520,952 + bioH BNILDI_02730 0.11 -0.2 520,953 - bioH BNILDI_02730 0.12 +0.6 520,953 - bioH BNILDI_02730 0.12 -0.5 520,953 - bioH BNILDI_02730 0.12 +0.5 521,027 + bioH BNILDI_02730 0.21 +1.8 521,027 + bioH BNILDI_02730 0.21 +0.3 521,027 + bioH BNILDI_02730 0.21 -1.0 521,027 + bioH BNILDI_02730 0.21 +0.0 521,028 - bioH BNILDI_02730 0.21 -0.4 521,028 - bioH BNILDI_02730 0.21 +0.2 521,028 - bioH BNILDI_02730 0.21 +0.0 521,106 + bioH BNILDI_02730 0.31 -1.1 521,197 + bioH BNILDI_02730 0.43 -2.7 521,302 - bioH BNILDI_02730 0.57 -0.4 521,514 + bioH BNILDI_02730 0.84 +0.4 521,515 - bioH BNILDI_02730 0.84 +0.3 521,515 - bioH BNILDI_02730 0.84 -0.4 521,527 - bioH BNILDI_02730 0.86 -0.3 521,527 - bioH BNILDI_02730 0.86 +0.2 521,628 + +0.2 521,628 + +0.6 521,657 + -0.2 521,671 + -0.9 521,671 + -0.4 521,671 + -0.9 521,671 + -0.2 521,671 + -0.1 521,671 + +1.3 521,671 + -0.9 521,671 + -1.5 521,672 - -0.2 521,672 - -0.1 521,672 - -0.0 521,672 - +1.9 521,676 + -1.5 521,677 - -4.3 521,683 - -0.2 521,691 + +0.8 521,692 - -0.1 521,770 + gntX BNILDI_02735 0.14 +0.2 521,771 - gntX BNILDI_02735 0.14 -0.7 521,778 + gntX BNILDI_02735 0.15 -0.2 521,880 - gntX BNILDI_02735 0.30 -0.8 521,965 - gntX BNILDI_02735 0.43 -0.8 521,977 - gntX BNILDI_02735 0.45 -0.4 522,121 + gntX BNILDI_02735 0.66 -1.3 522,239 - gntX BNILDI_02735 0.83 +0.5 522,410 + -2.6 522,410 + -1.0 522,541 - BNILDI_02740 0.22 +1.1 522,603 + BNILDI_02740 0.33 -0.1 522,616 + BNILDI_02740 0.35 -2.0 522,679 + BNILDI_02740 0.46 -1.3 522,680 - BNILDI_02740 0.46 -2.2 522,750 + BNILDI_02740 0.58 -1.0 522,750 + BNILDI_02740 0.58 -0.6 522,752 + BNILDI_02740 0.59 +0.1 522,981 + -1.8 522,981 + +0.3 522,984 + -0.7 522,984 + +0.1 522,987 + -0.3 522,988 - +0.9 522,988 - -0.2 522,988 - +0.2 522,988 - +0.3 523,117 + -0.1 523,118 - +1.3 523,118 - -2.9 523,118 - -1.5 523,235 - -1.4
Or see this region's nucleotide sequence