Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rng and yhdE overlap by 11 nucleotides yhdE and mreD are separated by 8 nucleotides mreD and mreC overlap by 1 nucleotides
BNILDI_01990: rng - ribonuclease G, at 393,393 to 394,862
rng
BNILDI_01995: yhdE - nucleoside triphosphate pyrophosphatase YhdE, at 394,852 to 395,445
yhdE
BNILDI_02000: mreD - rod shape-determining protein MreD, at 395,454 to 395,942
mreD
BNILDI_02005: mreC - rod shape-determining protein MreC, at 395,942 to 397,045
mreC
Position (kb)
394
395
396 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 393.858 kb on + strand, within rng at 394.011 kb on + strand, within rng at 394.012 kb on - strand, within rng at 394.012 kb on - strand, within rng at 394.012 kb on - strand, within rng at 394.084 kb on + strand, within rng at 394.117 kb on + strand, within rng at 394.118 kb on - strand, within rng at 394.147 kb on + strand, within rng at 394.147 kb on + strand, within rng at 394.148 kb on - strand, within rng at 394.275 kb on - strand, within rng at 394.275 kb on - strand, within rng at 394.275 kb on - strand, within rng at 394.275 kb on - strand, within rng at 394.430 kb on + strand, within rng at 394.431 kb on - strand, within rng at 394.467 kb on - strand, within rng at 394.689 kb on + strand, within rng at 394.706 kb on + strand, within rng at 394.717 kb on - strand at 394.725 kb on + strand at 394.726 kb on - strand at 394.738 kb on + strand at 394.752 kb on + strand at 394.808 kb on + strand at 394.842 kb on + strand at 394.843 kb on - strand at 394.904 kb on - strand at 394.904 kb on - strand at 394.946 kb on + strand, within yhdE at 394.946 kb on + strand, within yhdE at 394.957 kb on + strand, within yhdE at 394.957 kb on + strand, within yhdE at 394.958 kb on - strand, within yhdE at 394.958 kb on - strand, within yhdE at 394.958 kb on - strand, within yhdE at 394.958 kb on - strand, within yhdE at 395.036 kb on + strand, within yhdE at 395.232 kb on + strand, within yhdE at 395.315 kb on + strand, within yhdE at 395.315 kb on + strand, within yhdE at 395.315 kb on + strand, within yhdE at 395.316 kb on - strand, within yhdE at 395.316 kb on - strand, within yhdE at 395.361 kb on + strand, within yhdE at 395.367 kb on + strand, within yhdE at 395.429 kb on - strand at 395.429 kb on - strand at 395.429 kb on - strand at 395.433 kb on - strand at 395.456 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 393,858 + rng BNILDI_01990 0.32 -1.0 394,011 + rng BNILDI_01990 0.42 -0.2 394,012 - rng BNILDI_01990 0.42 +0.3 394,012 - rng BNILDI_01990 0.42 -0.7 394,012 - rng BNILDI_01990 0.42 -1.5 394,084 + rng BNILDI_01990 0.47 -1.5 394,117 + rng BNILDI_01990 0.49 -2.4 394,118 - rng BNILDI_01990 0.49 -0.1 394,147 + rng BNILDI_01990 0.51 -0.7 394,147 + rng BNILDI_01990 0.51 -0.8 394,148 - rng BNILDI_01990 0.51 -4.0 394,275 - rng BNILDI_01990 0.60 -1.6 394,275 - rng BNILDI_01990 0.60 -0.9 394,275 - rng BNILDI_01990 0.60 -0.3 394,275 - rng BNILDI_01990 0.60 -0.4 394,430 + rng BNILDI_01990 0.71 -0.7 394,431 - rng BNILDI_01990 0.71 -1.0 394,467 - rng BNILDI_01990 0.73 -0.9 394,689 + rng BNILDI_01990 0.88 -0.4 394,706 + rng BNILDI_01990 0.89 -0.3 394,717 - -0.9 394,725 + -2.1 394,726 - -0.6 394,738 + +0.3 394,752 + +1.4 394,808 + -3.1 394,842 + -1.1 394,843 - -0.0 394,904 - -1.1 394,904 - +0.2 394,946 + yhdE BNILDI_01995 0.16 -0.5 394,946 + yhdE BNILDI_01995 0.16 +1.2 394,957 + yhdE BNILDI_01995 0.18 -0.3 394,957 + yhdE BNILDI_01995 0.18 -0.5 394,958 - yhdE BNILDI_01995 0.18 +0.3 394,958 - yhdE BNILDI_01995 0.18 -0.5 394,958 - yhdE BNILDI_01995 0.18 +0.3 394,958 - yhdE BNILDI_01995 0.18 -0.1 395,036 + yhdE BNILDI_01995 0.31 -0.2 395,232 + yhdE BNILDI_01995 0.64 +0.3 395,315 + yhdE BNILDI_01995 0.78 -0.6 395,315 + yhdE BNILDI_01995 0.78 -0.2 395,315 + yhdE BNILDI_01995 0.78 +0.2 395,316 - yhdE BNILDI_01995 0.78 -0.2 395,316 - yhdE BNILDI_01995 0.78 -0.4 395,361 + yhdE BNILDI_01995 0.86 -0.5 395,367 + yhdE BNILDI_01995 0.87 +0.2 395,429 - +1.1 395,429 - +0.6 395,429 - +0.2 395,433 - -1.0 395,456 - -0.2
Or see this region's nucleotide sequence