Strain Fitness in Escherichia coli ECRC62 around BNILDI_01815

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntptsN and rapZ are separated by 45 nucleotidesrapZ and npr overlap by 4 nucleotidesnpr and yrbL are separated by 213 nucleotidesyrbL and mtgA overlap by 4 nucleotides BNILDI_01805: ptsN - PTS IIA-like nitrogen regulatory protein PtsN, at 350,952 to 351,443 ptsN BNILDI_01810: rapZ - RNase adapter RapZ, at 351,489 to 352,343 rapZ BNILDI_01815: npr - PTS phosphocarrier protein NPr, at 352,340 to 352,612 npr BNILDI_01820: yrbL - PhoP regulatory network protein YrbL, at 352,826 to 353,458 yrbL BNILDI_01825: mtgA - monofunctional biosynthetic peptidoglycan transglycosylase, at 353,455 to 354,183 mtgA Position (kb) 352 353Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 351.400 kb on - strandat 351.425 kb on + strandat 351.425 kb on + strandat 351.441 kb on + strandat 351.442 kb on - strandat 351.442 kb on - strandat 351.465 kb on - strandat 351.486 kb on + strandat 351.590 kb on - strand, within rapZat 351.665 kb on + strand, within rapZat 351.763 kb on + strand, within rapZat 351.763 kb on + strand, within rapZat 351.764 kb on - strand, within rapZat 352.023 kb on + strand, within rapZat 352.023 kb on + strand, within rapZat 352.024 kb on - strand, within rapZat 352.133 kb on - strand, within rapZat 352.142 kb on + strand, within rapZat 352.142 kb on + strand, within rapZat 352.142 kb on + strand, within rapZat 352.143 kb on - strand, within rapZat 352.171 kb on + strand, within rapZat 352.171 kb on + strand, within rapZat 352.177 kb on + strand, within rapZat 352.181 kb on + strand, within rapZat 352.181 kb on + strand, within rapZat 352.182 kb on - strand, within rapZat 352.182 kb on - strand, within rapZat 352.182 kb on - strand, within rapZat 352.202 kb on + strand, within rapZat 352.230 kb on - strand, within rapZat 352.254 kb on + strand, within rapZat 352.254 kb on + strand, within rapZat 352.255 kb on - strand, within rapZat 352.255 kb on - strand, within rapZat 352.278 kb on + strandat 352.279 kb on - strandat 352.319 kb on + strandat 352.417 kb on - strand, within nprat 352.417 kb on - strand, within nprat 352.446 kb on + strand, within nprat 352.447 kb on - strand, within nprat 352.447 kb on - strand, within nprat 352.450 kb on + strand, within nprat 352.487 kb on - strand, within nprat 352.588 kb on + strandat 352.631 kb on - strandat 352.631 kb on - strandat 352.631 kb on - strandat 352.749 kb on + strandat 352.750 kb on - strandat 352.789 kb on + strandat 352.923 kb on - strand, within yrbLat 352.923 kb on - strand, within yrbLat 352.923 kb on - strand, within yrbLat 353.102 kb on - strand, within yrbLat 353.184 kb on - strand, within yrbLat 353.368 kb on + strand, within yrbLat 353.369 kb on - strand, within yrbLat 353.501 kb on + strandat 353.501 kb on + strandat 353.501 kb on + strandat 353.502 kb on - strandat 353.581 kb on + strand, within mtgAat 353.584 kb on + strand, within mtgAat 353.584 kb on + strand, within mtgAat 353.584 kb on + strand, within mtgA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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351,400 - -2.3
351,425 + -0.1
351,425 + +0.3
351,441 + +0.8
351,442 - -0.4
351,442 - -1.4
351,465 - +1.7
351,486 + +0.3
351,590 - rapZ BNILDI_01810 0.12 +1.9
351,665 + rapZ BNILDI_01810 0.21 -0.7
351,763 + rapZ BNILDI_01810 0.32 -0.1
351,763 + rapZ BNILDI_01810 0.32 -1.5
351,764 - rapZ BNILDI_01810 0.32 -3.4
352,023 + rapZ BNILDI_01810 0.62 +0.6
352,023 + rapZ BNILDI_01810 0.62 +0.3
352,024 - rapZ BNILDI_01810 0.63 -0.6
352,133 - rapZ BNILDI_01810 0.75 -2.9
352,142 + rapZ BNILDI_01810 0.76 +1.5
352,142 + rapZ BNILDI_01810 0.76 +2.5
352,142 + rapZ BNILDI_01810 0.76 -0.1
352,143 - rapZ BNILDI_01810 0.76 +0.2
352,171 + rapZ BNILDI_01810 0.80 +0.9
352,171 + rapZ BNILDI_01810 0.80 -1.0
352,177 + rapZ BNILDI_01810 0.80 +0.6
352,181 + rapZ BNILDI_01810 0.81 -0.1
352,181 + rapZ BNILDI_01810 0.81 +1.0
352,182 - rapZ BNILDI_01810 0.81 +1.9
352,182 - rapZ BNILDI_01810 0.81 -0.1
352,182 - rapZ BNILDI_01810 0.81 -0.5
352,202 + rapZ BNILDI_01810 0.83 +1.0
352,230 - rapZ BNILDI_01810 0.87 -0.2
352,254 + rapZ BNILDI_01810 0.89 +0.9
352,254 + rapZ BNILDI_01810 0.89 +0.2
352,255 - rapZ BNILDI_01810 0.90 +0.5
352,255 - rapZ BNILDI_01810 0.90 +0.6
352,278 + -0.4
352,279 - +2.3
352,319 + -2.1
352,417 - npr BNILDI_01815 0.28 -0.1
352,417 - npr BNILDI_01815 0.28 +0.0
352,446 + npr BNILDI_01815 0.39 -0.1
352,447 - npr BNILDI_01815 0.39 -1.2
352,447 - npr BNILDI_01815 0.39 -0.7
352,450 + npr BNILDI_01815 0.40 -0.3
352,487 - npr BNILDI_01815 0.54 -0.2
352,588 + +0.5
352,631 - +0.1
352,631 - +0.2
352,631 - -0.3
352,749 + -0.3
352,750 - +0.0
352,789 + +0.6
352,923 - yrbL BNILDI_01820 0.15 -0.0
352,923 - yrbL BNILDI_01820 0.15 -0.4
352,923 - yrbL BNILDI_01820 0.15 +0.4
353,102 - yrbL BNILDI_01820 0.44 +1.1
353,184 - yrbL BNILDI_01820 0.57 -0.8
353,368 + yrbL BNILDI_01820 0.86 +0.1
353,369 - yrbL BNILDI_01820 0.86 +0.2
353,501 + -0.8
353,501 + -0.5
353,501 + +1.3
353,502 - -0.1
353,581 + mtgA BNILDI_01825 0.17 -0.3
353,584 + mtgA BNILDI_01825 0.18 -0.7
353,584 + mtgA BNILDI_01825 0.18 +0.3
353,584 + mtgA BNILDI_01825 0.18 -0.8

Or see this region's nucleotide sequence