Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24345

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaE and wcaF are separated by 15 nucleotideswcaF and OKFHMN_24345 are separated by 28 nucleotidesOKFHMN_24345 and fcl are separated by 2 nucleotidesfcl and gmm overlap by 1 nucleotides OKFHMN_24335: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 4,750,769 to 4,751,515 wcaE OKFHMN_24340: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 4,751,531 to 4,752,079 wcaF OKFHMN_24345: OKFHMN_24345 - GDP-mannose 4,6-dehydratase, at 4,752,108 to 4,753,229 _24345 OKFHMN_24350: fcl - GDP-L-fucose synthase, at 4,753,232 to 4,754,197 fcl OKFHMN_24355: gmm - GDP-mannose mannosyl hydrolase, at 4,754,197 to 4,754,679 gmm Position (kb) 4752 4753 4754Strain fitness (log2 ratio) -1 0 1at 4751.789 kb on + strand, within wcaFat 4751.843 kb on + strand, within wcaFat 4751.847 kb on + strand, within wcaFat 4752.093 kb on - strandat 4752.411 kb on - strand, within OKFHMN_24345at 4752.594 kb on + strand, within OKFHMN_24345at 4752.594 kb on + strand, within OKFHMN_24345at 4752.639 kb on + strand, within OKFHMN_24345at 4752.639 kb on + strand, within OKFHMN_24345at 4752.640 kb on - strand, within OKFHMN_24345at 4752.640 kb on - strand, within OKFHMN_24345at 4752.640 kb on - strand, within OKFHMN_24345at 4752.640 kb on - strand, within OKFHMN_24345at 4752.640 kb on - strand, within OKFHMN_24345at 4752.756 kb on + strand, within OKFHMN_24345at 4752.756 kb on + strand, within OKFHMN_24345at 4752.805 kb on - strand, within OKFHMN_24345at 4753.227 kb on + strandat 4753.227 kb on + strandat 4753.228 kb on - strandat 4753.409 kb on + strand, within fclat 4753.410 kb on - strand, within fclat 4753.680 kb on - strand, within fclat 4754.068 kb on + strand, within fclat 4754.156 kb on + strandat 4754.156 kb on + strandat 4754.207 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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4,751,789 + wcaF OKFHMN_24340 0.47 -0.2
4,751,843 + wcaF OKFHMN_24340 0.57 +0.5
4,751,847 + wcaF OKFHMN_24340 0.58 -0.3
4,752,093 - +1.2
4,752,411 - OKFHMN_24345 0.27 +0.5
4,752,594 + OKFHMN_24345 0.43 -0.1
4,752,594 + OKFHMN_24345 0.43 -1.1
4,752,639 + OKFHMN_24345 0.47 -0.5
4,752,639 + OKFHMN_24345 0.47 -0.0
4,752,640 - OKFHMN_24345 0.47 -0.2
4,752,640 - OKFHMN_24345 0.47 -0.5
4,752,640 - OKFHMN_24345 0.47 +0.0
4,752,640 - OKFHMN_24345 0.47 +1.5
4,752,640 - OKFHMN_24345 0.47 -0.9
4,752,756 + OKFHMN_24345 0.58 -0.1
4,752,756 + OKFHMN_24345 0.58 -0.5
4,752,805 - OKFHMN_24345 0.62 -0.2
4,753,227 + +0.1
4,753,227 + +0.1
4,753,228 - -1.4
4,753,409 + fcl OKFHMN_24350 0.18 +0.5
4,753,410 - fcl OKFHMN_24350 0.18 -1.5
4,753,680 - fcl OKFHMN_24350 0.46 +0.3
4,754,068 + fcl OKFHMN_24350 0.87 +0.3
4,754,156 + +0.4
4,754,156 + +0.0
4,754,207 + -0.2

Or see this region's nucleotide sequence