Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02260

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynaI and mppA are separated by 50 nucleotidesmppA and pgrR are separated by 335 nucleotides OKFHMN_02255: ynaI - low conductance mechanosensitive channel YnaI, at 339,636 to 340,667 ynaI OKFHMN_02260: mppA - murein tripeptide ABC transporter substrate-binding protein MppA, at 340,718 to 342,331 mppA OKFHMN_02265: pgrR - DNA-binding transcriptional regulator PgrR, at 342,667 to 343,566 pgrR Position (kb) 340 341 342 343Strain fitness (log2 ratio) -3 -2 -1 0 1at 339.819 kb on - strand, within ynaIat 340.456 kb on + strand, within ynaIat 340.629 kb on + strandat 340.735 kb on - strandat 340.816 kb on + strandat 340.983 kb on - strand, within mppAat 341.878 kb on + strand, within mppAat 342.344 kb on - strandat 342.366 kb on + strandat 342.430 kb on - strandat 342.653 kb on - strandat 342.889 kb on - strand, within pgrRat 343.078 kb on - strand, within pgrRat 343.104 kb on + strand, within pgrRat 343.264 kb on + strand, within pgrRat 343.265 kb on - strand, within pgrR

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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339,819 - ynaI OKFHMN_02255 0.18 +0.3
340,456 + ynaI OKFHMN_02255 0.79 -0.7
340,629 + -2.4
340,735 - -0.5
340,816 + -3.4
340,983 - mppA OKFHMN_02260 0.16 -0.4
341,878 + mppA OKFHMN_02260 0.72 +0.6
342,344 - -1.3
342,366 + +0.8
342,430 - +0.7
342,653 - -0.1
342,889 - pgrR OKFHMN_02265 0.25 -1.1
343,078 - pgrR OKFHMN_02265 0.46 -1.0
343,104 + pgrR OKFHMN_02265 0.49 -0.1
343,264 + pgrR OKFHMN_02265 0.66 -0.2
343,265 - pgrR OKFHMN_02265 0.66 -0.0

Or see this region's nucleotide sequence