Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnp-IS3 and tra5 overlap by 1 nucleotides tra5 and abgT are separated by 110 nucleotides abgT and ogt are separated by 10 nucleotides ogt and fnr are separated by 194 nucleotides
OKFHMN_02220: tnp-IS3 - IS3 family transposase, at 334,731 to 335,057
tnp-IS3
OKFHMN_02225: tra5 - IS3 family transposase, at 335,057 to 335,707
tra5
OKFHMN_02230: abgT - p-aminobenzoyl-glutamate transporter, at 335,818 to 336,510
abgT
OKFHMN_02235: ogt - methylated-DNA--[protein]-cysteine S-methyltransferase, at 336,521 to 337,036
ogt
OKFHMN_02240: fnr - fumarate/nitrate reduction transcriptional regulator Fnr, at 337,231 to 337,983
fnr
Position (kb) 335
336
337 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 335.768 kb on + strand at 335.774 kb on + strand at 335.775 kb on - strand at 336.249 kb on - strand, within abgT at 336.392 kb on - strand, within abgT at 336.748 kb on + strand, within ogt at 336.748 kb on + strand, within ogt at 337.165 kb on + strand at 337.230 kb on + strand at 337.230 kb on + strand at 337.251 kb on + strand at 337.383 kb on + strand, within fnr at 337.467 kb on + strand, within fnr
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 335,768 + +0.9 335,774 + +1.2 335,775 - +0.3 336,249 - abgT OKFHMN_02230 0.62 -1.1 336,392 - abgT OKFHMN_02230 0.83 -1.3 336,748 + ogt OKFHMN_02235 0.44 -3.4 336,748 + ogt OKFHMN_02235 0.44 +1.8 337,165 + +0.8 337,230 + +0.1 337,230 + -1.8 337,251 + +0.0 337,383 + fnr OKFHMN_02240 0.20 +0.7 337,467 + fnr OKFHMN_02240 0.31 +0.1
Or see this region's nucleotide sequence