Experiment: Seed; RBT.2.2.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GEMAOFIL_02000 and GEMAOFIL_02001 are separated by 66 nucleotides GEMAOFIL_02001 and GEMAOFIL_02002 overlap by 4 nucleotides GEMAOFIL_02002 and GEMAOFIL_02003 are separated by 122 nucleotides
GEMAOFIL_02000: GEMAOFIL_02000 - 4-methylaminobutanoate oxidase (formaldehyde-forming), at 2,202,269 to 2,203,561
_02000
GEMAOFIL_02001: GEMAOFIL_02001 - 2-(acetamidomethylene)succinate hydrolase, at 2,203,628 to 2,204,476
_02001
GEMAOFIL_02002: GEMAOFIL_02002 - Bicyclomycin resistance protein, at 2,204,473 to 2,205,669
_02002
GEMAOFIL_02003: GEMAOFIL_02003 - Gamma-glutamylputrescine oxidoreductase, at 2,205,792 to 2,207,099
_02003
Position (kb)
2204
2205
2206 Strain fitness (log2 ratio)
-2
-1
0
1 at 2203.740 kb on - strand, within GEMAOFIL_02001 at 2203.785 kb on - strand, within GEMAOFIL_02001 at 2203.793 kb on + strand, within GEMAOFIL_02001 at 2203.793 kb on + strand, within GEMAOFIL_02001 at 2203.794 kb on - strand, within GEMAOFIL_02001 at 2203.794 kb on - strand, within GEMAOFIL_02001 at 2203.794 kb on - strand, within GEMAOFIL_02001 at 2203.794 kb on - strand, within GEMAOFIL_02001 at 2203.794 kb on - strand, within GEMAOFIL_02001 at 2203.794 kb on - strand, within GEMAOFIL_02001 at 2203.847 kb on + strand, within GEMAOFIL_02001 at 2203.848 kb on - strand, within GEMAOFIL_02001 at 2203.862 kb on + strand, within GEMAOFIL_02001 at 2203.863 kb on - strand, within GEMAOFIL_02001 at 2203.863 kb on - strand, within GEMAOFIL_02001 at 2203.863 kb on - strand, within GEMAOFIL_02001 at 2203.863 kb on - strand, within GEMAOFIL_02001 at 2204.396 kb on + strand at 2204.397 kb on - strand at 2204.397 kb on - strand at 2204.541 kb on + strand at 2204.542 kb on - strand at 2204.542 kb on - strand at 2204.545 kb on + strand at 2204.546 kb on - strand at 2204.546 kb on - strand at 2204.714 kb on - strand, within GEMAOFIL_02002 at 2204.714 kb on - strand, within GEMAOFIL_02002 at 2204.714 kb on - strand, within GEMAOFIL_02002 at 2204.841 kb on - strand, within GEMAOFIL_02002 at 2204.937 kb on - strand, within GEMAOFIL_02002 at 2205.074 kb on - strand, within GEMAOFIL_02002 at 2205.074 kb on - strand, within GEMAOFIL_02002 at 2205.154 kb on - strand, within GEMAOFIL_02002 at 2205.176 kb on - strand, within GEMAOFIL_02002 at 2205.185 kb on - strand, within GEMAOFIL_02002 at 2205.358 kb on + strand, within GEMAOFIL_02002 at 2205.406 kb on + strand, within GEMAOFIL_02002 at 2205.406 kb on + strand, within GEMAOFIL_02002 at 2205.406 kb on + strand, within GEMAOFIL_02002 at 2205.406 kb on + strand, within GEMAOFIL_02002 at 2205.407 kb on - strand, within GEMAOFIL_02002 at 2205.619 kb on + strand at 2205.772 kb on + strand at 2205.775 kb on + strand at 2205.783 kb on + strand at 2205.783 kb on + strand at 2205.791 kb on + strand at 2205.791 kb on + strand at 2205.823 kb on - strand at 2205.826 kb on - strand at 2205.826 kb on - strand at 2205.826 kb on - strand at 2205.840 kb on - strand at 2205.840 kb on - strand at 2205.843 kb on + strand at 2205.950 kb on + strand, within GEMAOFIL_02003 at 2205.977 kb on + strand, within GEMAOFIL_02003 at 2206.174 kb on - strand, within GEMAOFIL_02003 at 2206.179 kb on + strand, within GEMAOFIL_02003 at 2206.311 kb on + strand, within GEMAOFIL_02003 at 2206.311 kb on + strand, within GEMAOFIL_02003 at 2206.311 kb on + strand, within GEMAOFIL_02003 at 2206.311 kb on + strand, within GEMAOFIL_02003 at 2206.312 kb on - strand, within GEMAOFIL_02003 at 2206.346 kb on + strand, within GEMAOFIL_02003 at 2206.347 kb on - strand, within GEMAOFIL_02003 at 2206.443 kb on + strand, within GEMAOFIL_02003 at 2206.443 kb on + strand, within GEMAOFIL_02003 at 2206.444 kb on - strand, within GEMAOFIL_02003 at 2206.444 kb on - strand, within GEMAOFIL_02003 at 2206.444 kb on - strand, within GEMAOFIL_02003 at 2206.444 kb on - strand, within GEMAOFIL_02003 at 2206.567 kb on - strand, within GEMAOFIL_02003 at 2206.669 kb on - strand, within GEMAOFIL_02003
Per-strain Table
Position Strand Gene LocusTag Fraction Seed; RBT.2.2.5 remove 2,203,740 - GEMAOFIL_02001 0.13 +0.0 2,203,785 - GEMAOFIL_02001 0.18 +0.1 2,203,793 + GEMAOFIL_02001 0.19 -0.2 2,203,793 + GEMAOFIL_02001 0.19 -0.5 2,203,794 - GEMAOFIL_02001 0.20 +1.0 2,203,794 - GEMAOFIL_02001 0.20 -1.4 2,203,794 - GEMAOFIL_02001 0.20 -0.5 2,203,794 - GEMAOFIL_02001 0.20 -0.7 2,203,794 - GEMAOFIL_02001 0.20 -0.8 2,203,794 - GEMAOFIL_02001 0.20 -0.7 2,203,847 + GEMAOFIL_02001 0.26 +0.0 2,203,848 - GEMAOFIL_02001 0.26 +0.0 2,203,862 + GEMAOFIL_02001 0.28 +0.3 2,203,863 - GEMAOFIL_02001 0.28 +0.4 2,203,863 - GEMAOFIL_02001 0.28 +0.6 2,203,863 - GEMAOFIL_02001 0.28 +0.2 2,203,863 - GEMAOFIL_02001 0.28 +0.3 2,204,396 + +0.6 2,204,397 - +0.0 2,204,397 - +0.1 2,204,541 + -0.3 2,204,542 - +0.0 2,204,542 - -0.3 2,204,545 + +0.2 2,204,546 - +0.2 2,204,546 - -0.6 2,204,714 - GEMAOFIL_02002 0.20 -0.8 2,204,714 - GEMAOFIL_02002 0.20 +0.2 2,204,714 - GEMAOFIL_02002 0.20 -0.6 2,204,841 - GEMAOFIL_02002 0.31 +0.6 2,204,937 - GEMAOFIL_02002 0.39 -1.2 2,205,074 - GEMAOFIL_02002 0.50 -0.8 2,205,074 - GEMAOFIL_02002 0.50 -0.6 2,205,154 - GEMAOFIL_02002 0.57 -1.4 2,205,176 - GEMAOFIL_02002 0.59 +0.1 2,205,185 - GEMAOFIL_02002 0.59 -0.8 2,205,358 + GEMAOFIL_02002 0.74 +0.2 2,205,406 + GEMAOFIL_02002 0.78 +0.3 2,205,406 + GEMAOFIL_02002 0.78 -0.0 2,205,406 + GEMAOFIL_02002 0.78 -0.6 2,205,406 + GEMAOFIL_02002 0.78 -0.1 2,205,407 - GEMAOFIL_02002 0.78 -0.9 2,205,619 + +0.1 2,205,772 + -0.1 2,205,775 + -0.4 2,205,783 + +0.6 2,205,783 + -0.2 2,205,791 + -1.3 2,205,791 + +0.7 2,205,823 - -0.5 2,205,826 - -0.8 2,205,826 - -0.4 2,205,826 - -0.2 2,205,840 - -0.1 2,205,840 - +0.6 2,205,843 + +1.5 2,205,950 + GEMAOFIL_02003 0.12 -0.1 2,205,977 + GEMAOFIL_02003 0.14 -0.6 2,206,174 - GEMAOFIL_02003 0.29 -0.5 2,206,179 + GEMAOFIL_02003 0.30 +0.2 2,206,311 + GEMAOFIL_02003 0.40 -1.1 2,206,311 + GEMAOFIL_02003 0.40 -0.5 2,206,311 + GEMAOFIL_02003 0.40 -0.1 2,206,311 + GEMAOFIL_02003 0.40 -0.1 2,206,312 - GEMAOFIL_02003 0.40 +0.7 2,206,346 + GEMAOFIL_02003 0.42 -0.5 2,206,347 - GEMAOFIL_02003 0.42 -0.5 2,206,443 + GEMAOFIL_02003 0.50 +1.1 2,206,443 + GEMAOFIL_02003 0.50 -0.4 2,206,444 - GEMAOFIL_02003 0.50 +0.3 2,206,444 - GEMAOFIL_02003 0.50 +0.2 2,206,444 - GEMAOFIL_02003 0.50 -0.2 2,206,444 - GEMAOFIL_02003 0.50 +1.4 2,206,567 - GEMAOFIL_02003 0.59 -0.4 2,206,669 - GEMAOFIL_02003 0.67 -1.9
Or see this region's nucleotide sequence