Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prmB and aroC are separated by 34 nucleotides aroC and mepA are separated by 2 nucleotides mepA and yfcA overlap by 1 nucleotides
HEPCGN_21815: prmB - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 3,119,028 to 3,119,960
prmB
HEPCGN_21820: aroC - chorismate synthase, at 3,119,995 to 3,121,080
aroC
HEPCGN_21825: mepA - penicillin-insensitive murein endopeptidase, at 3,121,083 to 3,121,907
mepA
HEPCGN_21830: yfcA - putative membrane transporter protein YfcA, at 3,121,907 to 3,122,716
yfcA
Position (kb) 3119
3120
3121
3122 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 3119.025 kb on + strand at 3119.033 kb on + strand at 3119.213 kb on + strand, within prmB at 3119.301 kb on + strand, within prmB at 3119.432 kb on + strand, within prmB at 3119.437 kb on + strand, within prmB at 3119.441 kb on + strand, within prmB at 3119.441 kb on + strand, within prmB at 3119.442 kb on - strand, within prmB at 3119.481 kb on + strand, within prmB at 3119.482 kb on - strand, within prmB at 3119.672 kb on + strand, within prmB at 3119.672 kb on + strand, within prmB at 3119.827 kb on + strand, within prmB at 3119.828 kb on - strand, within prmB at 3119.834 kb on + strand, within prmB at 3119.844 kb on + strand, within prmB at 3119.951 kb on + strand at 3119.952 kb on - strand at 3119.962 kb on + strand at 3120.279 kb on + strand, within aroC at 3120.279 kb on + strand, within aroC at 3120.326 kb on - strand, within aroC at 3120.444 kb on + strand, within aroC at 3121.065 kb on + strand at 3121.192 kb on + strand, within mepA at 3121.206 kb on - strand, within mepA at 3121.214 kb on + strand, within mepA at 3121.215 kb on - strand, within mepA at 3121.255 kb on - strand, within mepA at 3121.269 kb on - strand, within mepA at 3121.313 kb on + strand, within mepA at 3121.373 kb on + strand, within mepA at 3121.676 kb on + strand, within mepA at 3121.709 kb on + strand, within mepA at 3121.717 kb on + strand, within mepA at 3121.717 kb on + strand, within mepA at 3121.723 kb on + strand, within mepA at 3121.905 kb on + strand at 3121.906 kb on - strand at 3121.919 kb on - strand at 3121.919 kb on - strand at 3121.921 kb on + strand at 3121.921 kb on + strand at 3122.074 kb on + strand, within yfcA
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 3,119,025 + -0.7 3,119,033 + -2.9 3,119,213 + prmB HEPCGN_21815 0.20 -1.3 3,119,301 + prmB HEPCGN_21815 0.29 -0.6 3,119,432 + prmB HEPCGN_21815 0.43 -1.9 3,119,437 + prmB HEPCGN_21815 0.44 -1.7 3,119,441 + prmB HEPCGN_21815 0.44 -1.5 3,119,441 + prmB HEPCGN_21815 0.44 +1.5 3,119,442 - prmB HEPCGN_21815 0.44 -0.9 3,119,481 + prmB HEPCGN_21815 0.49 -1.2 3,119,482 - prmB HEPCGN_21815 0.49 -2.3 3,119,672 + prmB HEPCGN_21815 0.69 -0.0 3,119,672 + prmB HEPCGN_21815 0.69 -0.1 3,119,827 + prmB HEPCGN_21815 0.86 +0.4 3,119,828 - prmB HEPCGN_21815 0.86 -1.3 3,119,834 + prmB HEPCGN_21815 0.86 +0.1 3,119,844 + prmB HEPCGN_21815 0.87 +0.1 3,119,951 + +1.1 3,119,952 - -3.2 3,119,962 + +0.7 3,120,279 + aroC HEPCGN_21820 0.26 -3.3 3,120,279 + aroC HEPCGN_21820 0.26 -4.8 3,120,326 - aroC HEPCGN_21820 0.30 -4.0 3,120,444 + aroC HEPCGN_21820 0.41 -3.2 3,121,065 + -0.3 3,121,192 + mepA HEPCGN_21825 0.13 -1.3 3,121,206 - mepA HEPCGN_21825 0.15 +0.0 3,121,214 + mepA HEPCGN_21825 0.16 +0.3 3,121,215 - mepA HEPCGN_21825 0.16 -1.5 3,121,255 - mepA HEPCGN_21825 0.21 -0.6 3,121,269 - mepA HEPCGN_21825 0.23 +0.2 3,121,313 + mepA HEPCGN_21825 0.28 -0.0 3,121,373 + mepA HEPCGN_21825 0.35 +0.2 3,121,676 + mepA HEPCGN_21825 0.72 +0.7 3,121,709 + mepA HEPCGN_21825 0.76 +2.1 3,121,717 + mepA HEPCGN_21825 0.77 -0.8 3,121,717 + mepA HEPCGN_21825 0.77 -2.1 3,121,723 + mepA HEPCGN_21825 0.78 +0.4 3,121,905 + -0.4 3,121,906 - -1.3 3,121,919 - +0.2 3,121,919 - -0.2 3,121,921 + -1.3 3,121,921 + +0.3 3,122,074 + yfcA HEPCGN_21830 0.21 +0.2
Or see this region's nucleotide sequence