Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potB and potC overlap by 4 nucleotides potC and potD overlap by 4 nucleotides potD and NOLOHH_20955 are separated by 57 nucleotides NOLOHH_20955 and ycfZ overlap by 4 nucleotides
NOLOHH_20940: potB - spermidine/putrescine ABC transporter permease PotB, at 4,119,585 to 4,120,442
potB
NOLOHH_20945: potC - spermidine/putrescine ABC transporter permease PotC, at 4,120,439 to 4,121,233
potC
NOLOHH_20950: potD - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 4,121,230 to 4,122,276
potD
NOLOHH_20955: NOLOHH_20955 - DUF3592 domain-containing protein, at 4,122,334 to 4,122,795
_20955
NOLOHH_20960: ycfZ - Inner membrane protein YcfZ, at 4,122,792 to 4,123,580
ycfZ
Position (kb) 4121
4122
4123 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4120.251 kb on + strand, within potB at 4120.358 kb on + strand at 4120.358 kb on + strand at 4120.358 kb on + strand at 4120.493 kb on + strand at 4120.608 kb on + strand, within potC at 4120.843 kb on + strand, within potC at 4120.843 kb on + strand, within potC at 4121.010 kb on + strand, within potC at 4121.037 kb on + strand, within potC at 4121.037 kb on + strand, within potC at 4121.037 kb on + strand, within potC at 4121.069 kb on + strand, within potC at 4121.069 kb on + strand, within potC at 4121.069 kb on + strand, within potC at 4121.145 kb on + strand, within potC at 4121.145 kb on + strand, within potC at 4121.148 kb on + strand, within potC at 4121.172 kb on + strand at 4121.172 kb on + strand at 4121.188 kb on + strand at 4121.212 kb on + strand at 4121.317 kb on + strand at 4121.391 kb on + strand, within potD at 4121.395 kb on + strand, within potD at 4121.395 kb on + strand, within potD at 4121.425 kb on + strand, within potD at 4121.425 kb on + strand, within potD at 4121.482 kb on + strand, within potD at 4121.482 kb on + strand, within potD at 4121.482 kb on + strand, within potD at 4121.485 kb on + strand, within potD at 4121.616 kb on + strand, within potD at 4121.815 kb on + strand, within potD at 4122.132 kb on + strand, within potD at 4122.238 kb on + strand at 4122.238 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,120,251 + potB NOLOHH_20940 0.78 +2.0 4,120,358 + -0.7 4,120,358 + +3.2 4,120,358 + -1.1 4,120,493 + -2.5 4,120,608 + potC NOLOHH_20945 0.21 -0.9 4,120,843 + potC NOLOHH_20945 0.51 -0.3 4,120,843 + potC NOLOHH_20945 0.51 +3.0 4,121,010 + potC NOLOHH_20945 0.72 -2.6 4,121,037 + potC NOLOHH_20945 0.75 +0.7 4,121,037 + potC NOLOHH_20945 0.75 -0.1 4,121,037 + potC NOLOHH_20945 0.75 -1.5 4,121,069 + potC NOLOHH_20945 0.79 -0.2 4,121,069 + potC NOLOHH_20945 0.79 -2.3 4,121,069 + potC NOLOHH_20945 0.79 +0.0 4,121,145 + potC NOLOHH_20945 0.89 +2.0 4,121,145 + potC NOLOHH_20945 0.89 +1.4 4,121,148 + potC NOLOHH_20945 0.89 -1.0 4,121,172 + -0.4 4,121,172 + -2.5 4,121,188 + +0.4 4,121,212 + -0.3 4,121,317 + +1.3 4,121,391 + potD NOLOHH_20950 0.15 -1.1 4,121,395 + potD NOLOHH_20950 0.16 -2.5 4,121,395 + potD NOLOHH_20950 0.16 +0.8 4,121,425 + potD NOLOHH_20950 0.19 -0.3 4,121,425 + potD NOLOHH_20950 0.19 -0.8 4,121,482 + potD NOLOHH_20950 0.24 -2.6 4,121,482 + potD NOLOHH_20950 0.24 -2.7 4,121,482 + potD NOLOHH_20950 0.24 -1.7 4,121,485 + potD NOLOHH_20950 0.24 +0.7 4,121,616 + potD NOLOHH_20950 0.37 -1.5 4,121,815 + potD NOLOHH_20950 0.56 -2.8 4,122,132 + potD NOLOHH_20950 0.86 -0.5 4,122,238 + -0.8 4,122,238 + +1.2
Or see this region's nucleotide sequence