Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisB and hisC overlap by 1 nucleotides hisC and hisD overlap by 4 nucleotides
NOLOHH_15820: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 3,118,655 to 3,119,722
hisB
NOLOHH_15825: hisC - histidinol-phosphate transaminase, at 3,119,722 to 3,120,792
hisC
NOLOHH_15830: hisD - Histidinol dehydrogenase, at 3,120,789 to 3,122,093
hisD
Position (kb)
3119
3120
3121 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3118.766 kb on - strand, within hisB at 3118.880 kb on - strand, within hisB at 3118.880 kb on - strand, within hisB at 3118.880 kb on - strand, within hisB at 3118.907 kb on - strand, within hisB at 3119.050 kb on - strand, within hisB at 3119.141 kb on - strand, within hisB at 3119.346 kb on + strand, within hisB at 3119.346 kb on + strand, within hisB at 3119.347 kb on - strand, within hisB at 3119.741 kb on - strand at 3119.818 kb on - strand at 3119.831 kb on - strand, within hisC at 3119.860 kb on - strand, within hisC at 3119.898 kb on - strand, within hisC at 3119.961 kb on - strand, within hisC at 3120.166 kb on + strand, within hisC at 3120.233 kb on + strand, within hisC at 3120.234 kb on - strand, within hisC at 3120.234 kb on - strand, within hisC at 3120.341 kb on + strand, within hisC at 3120.341 kb on + strand, within hisC at 3120.342 kb on - strand, within hisC at 3120.382 kb on + strand, within hisC at 3120.575 kb on - strand, within hisC at 3120.734 kb on + strand at 3120.735 kb on - strand at 3120.777 kb on + strand at 3120.778 kb on - strand at 3120.804 kb on - strand at 3120.926 kb on + strand, within hisD at 3120.926 kb on + strand, within hisD at 3120.980 kb on - strand, within hisD at 3120.980 kb on - strand, within hisD at 3120.990 kb on + strand, within hisD at 3121.353 kb on - strand, within hisD at 3121.548 kb on - strand, within hisD at 3121.666 kb on - strand, within hisD at 3121.669 kb on - strand, within hisD at 3121.669 kb on - strand, within hisD at 3121.669 kb on - strand, within hisD at 3121.704 kb on - strand, within hisD at 3121.705 kb on + strand, within hisD at 3121.705 kb on + strand, within hisD at 3121.706 kb on - strand, within hisD at 3121.706 kb on - strand, within hisD at 3121.706 kb on - strand, within hisD at 3121.706 kb on - strand, within hisD at 3121.767 kb on + strand, within hisD at 3121.768 kb on - strand, within hisD at 3121.768 kb on - strand, within hisD at 3121.768 kb on - strand, within hisD
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,118,766 - hisB NOLOHH_15820 0.10 -2.1 3,118,880 - hisB NOLOHH_15820 0.21 +1.5 3,118,880 - hisB NOLOHH_15820 0.21 +0.4 3,118,880 - hisB NOLOHH_15820 0.21 -1.8 3,118,907 - hisB NOLOHH_15820 0.24 +0.5 3,119,050 - hisB NOLOHH_15820 0.37 -3.1 3,119,141 - hisB NOLOHH_15820 0.46 -0.9 3,119,346 + hisB NOLOHH_15820 0.65 -3.0 3,119,346 + hisB NOLOHH_15820 0.65 -1.0 3,119,347 - hisB NOLOHH_15820 0.65 -1.4 3,119,741 - +0.7 3,119,818 - -1.6 3,119,831 - hisC NOLOHH_15825 0.10 -0.9 3,119,860 - hisC NOLOHH_15825 0.13 +1.5 3,119,898 - hisC NOLOHH_15825 0.16 -2.2 3,119,961 - hisC NOLOHH_15825 0.22 +1.2 3,120,166 + hisC NOLOHH_15825 0.41 +0.7 3,120,233 + hisC NOLOHH_15825 0.48 +1.0 3,120,234 - hisC NOLOHH_15825 0.48 +0.1 3,120,234 - hisC NOLOHH_15825 0.48 +0.9 3,120,341 + hisC NOLOHH_15825 0.58 -0.9 3,120,341 + hisC NOLOHH_15825 0.58 -1.9 3,120,342 - hisC NOLOHH_15825 0.58 +0.7 3,120,382 + hisC NOLOHH_15825 0.62 -2.7 3,120,575 - hisC NOLOHH_15825 0.80 -0.4 3,120,734 + +0.0 3,120,735 - -1.6 3,120,777 + -1.2 3,120,778 - -0.9 3,120,804 - -2.3 3,120,926 + hisD NOLOHH_15830 0.10 +1.0 3,120,926 + hisD NOLOHH_15830 0.10 -3.3 3,120,980 - hisD NOLOHH_15830 0.15 -0.5 3,120,980 - hisD NOLOHH_15830 0.15 -0.7 3,120,990 + hisD NOLOHH_15830 0.15 +0.7 3,121,353 - hisD NOLOHH_15830 0.43 +0.8 3,121,548 - hisD NOLOHH_15830 0.58 -0.6 3,121,666 - hisD NOLOHH_15830 0.67 +0.9 3,121,669 - hisD NOLOHH_15830 0.67 +1.3 3,121,669 - hisD NOLOHH_15830 0.67 +0.9 3,121,669 - hisD NOLOHH_15830 0.67 -2.9 3,121,704 - hisD NOLOHH_15830 0.70 +0.7 3,121,705 + hisD NOLOHH_15830 0.70 -0.4 3,121,705 + hisD NOLOHH_15830 0.70 -1.8 3,121,706 - hisD NOLOHH_15830 0.70 +0.1 3,121,706 - hisD NOLOHH_15830 0.70 -0.2 3,121,706 - hisD NOLOHH_15830 0.70 +1.1 3,121,706 - hisD NOLOHH_15830 0.70 +0.6 3,121,767 + hisD NOLOHH_15830 0.75 -1.1 3,121,768 - hisD NOLOHH_15830 0.75 -0.0 3,121,768 - hisD NOLOHH_15830 0.75 +0.8 3,121,768 - hisD NOLOHH_15830 0.75 -0.7
Or see this region's nucleotide sequence