Strain Fitness in Escherichia coli ECOR27 around NOLOHH_10435

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnfeF and Ile2_trna are separated by 53 nucleotidesIle2_trna and mug are separated by 124 nucleotidesmug and rpoD are separated by 77 nucleotides NOLOHH_10430: nfeF - NADPH-dependent ferric chelate reductase, at 2,016,326 to 2,017,090 nfeF NOLOHH_10435: Ile2_trna - tRNA-Ile2(cat), at 2,017,144 to 2,017,219 _trna NOLOHH_10440: mug - G/U mismatch-specific DNA glycosylase, at 2,017,344 to 2,017,850 mug NOLOHH_10445: rpoD - RNA polymerase sigma factor RpoD, at 2,017,928 to 2,019,769 rpoD Position (kb) 2017 2018Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2016.192 kb on + strandat 2016.193 kb on - strandat 2016.193 kb on - strandat 2016.215 kb on - strandat 2016.223 kb on + strandat 2016.223 kb on + strandat 2016.224 kb on - strandat 2016.224 kb on - strandat 2016.224 kb on - strandat 2016.296 kb on + strandat 2016.460 kb on + strand, within nfeFat 2016.460 kb on + strand, within nfeFat 2016.460 kb on + strand, within nfeFat 2016.460 kb on + strand, within nfeFat 2016.461 kb on - strand, within nfeFat 2016.587 kb on + strand, within nfeFat 2016.588 kb on - strand, within nfeFat 2016.589 kb on + strand, within nfeFat 2016.609 kb on + strand, within nfeFat 2016.609 kb on + strand, within nfeFat 2016.609 kb on + strand, within nfeFat 2016.610 kb on - strand, within nfeFat 2016.610 kb on - strand, within nfeFat 2016.610 kb on - strand, within nfeFat 2016.698 kb on - strand, within nfeFat 2016.755 kb on + strand, within nfeFat 2016.755 kb on + strand, within nfeFat 2016.756 kb on - strand, within nfeFat 2016.836 kb on - strand, within nfeFat 2016.898 kb on + strand, within nfeFat 2016.899 kb on - strand, within nfeFat 2016.899 kb on - strand, within nfeFat 2016.974 kb on + strand, within nfeFat 2016.974 kb on + strand, within nfeFat 2016.975 kb on - strand, within nfeFat 2016.975 kb on - strand, within nfeFat 2017.095 kb on + strandat 2017.098 kb on - strandat 2017.099 kb on + strandat 2017.138 kb on + strandat 2017.138 kb on + strandat 2017.139 kb on - strandat 2017.139 kb on - strandat 2017.139 kb on - strandat 2017.139 kb on - strandat 2017.295 kb on + strandat 2017.312 kb on - strandat 2017.312 kb on - strandat 2017.329 kb on + strandat 2017.330 kb on - strandat 2017.330 kb on - strandat 2017.330 kb on - strandat 2017.339 kb on + strandat 2017.339 kb on + strandat 2017.343 kb on + strandat 2017.355 kb on + strandat 2017.356 kb on - strandat 2017.465 kb on + strand, within mugat 2017.466 kb on - strand, within mugat 2017.467 kb on + strand, within mugat 2017.467 kb on + strand, within mugat 2017.467 kb on + strand, within mugat 2017.467 kb on + strand, within mugat 2017.467 kb on + strand, within mugat 2017.468 kb on - strand, within mugat 2017.638 kb on + strand, within mugat 2017.774 kb on + strand, within mug

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,016,192 + +2.0
2,016,193 - -0.8
2,016,193 - +0.5
2,016,215 - -0.2
2,016,223 + +0.1
2,016,223 + +0.9
2,016,224 - +1.0
2,016,224 - +0.1
2,016,224 - +0.7
2,016,296 + +1.1
2,016,460 + nfeF NOLOHH_10430 0.18 -0.7
2,016,460 + nfeF NOLOHH_10430 0.18 -3.0
2,016,460 + nfeF NOLOHH_10430 0.18 -1.3
2,016,460 + nfeF NOLOHH_10430 0.18 +0.4
2,016,461 - nfeF NOLOHH_10430 0.18 -2.7
2,016,587 + nfeF NOLOHH_10430 0.34 -3.4
2,016,588 - nfeF NOLOHH_10430 0.34 -0.1
2,016,589 + nfeF NOLOHH_10430 0.34 -0.6
2,016,609 + nfeF NOLOHH_10430 0.37 -0.3
2,016,609 + nfeF NOLOHH_10430 0.37 -0.1
2,016,609 + nfeF NOLOHH_10430 0.37 +0.5
2,016,610 - nfeF NOLOHH_10430 0.37 -1.6
2,016,610 - nfeF NOLOHH_10430 0.37 +1.4
2,016,610 - nfeF NOLOHH_10430 0.37 +0.7
2,016,698 - nfeF NOLOHH_10430 0.49 +0.3
2,016,755 + nfeF NOLOHH_10430 0.56 +1.0
2,016,755 + nfeF NOLOHH_10430 0.56 +1.2
2,016,756 - nfeF NOLOHH_10430 0.56 +2.9
2,016,836 - nfeF NOLOHH_10430 0.67 +0.9
2,016,898 + nfeF NOLOHH_10430 0.75 +0.4
2,016,899 - nfeF NOLOHH_10430 0.75 -1.2
2,016,899 - nfeF NOLOHH_10430 0.75 +1.4
2,016,974 + nfeF NOLOHH_10430 0.85 +1.0
2,016,974 + nfeF NOLOHH_10430 0.85 +0.8
2,016,975 - nfeF NOLOHH_10430 0.85 +0.8
2,016,975 - nfeF NOLOHH_10430 0.85 +0.9
2,017,095 + +0.8
2,017,098 - +0.2
2,017,099 + +1.5
2,017,138 + -1.0
2,017,138 + +0.7
2,017,139 - +0.1
2,017,139 - -0.2
2,017,139 - +0.9
2,017,139 - -0.1
2,017,295 + +0.1
2,017,312 - -0.2
2,017,312 - -3.3
2,017,329 + -0.7
2,017,330 - +0.7
2,017,330 - +0.8
2,017,330 - +0.2
2,017,339 + -3.2
2,017,339 + -1.6
2,017,343 + -3.1
2,017,355 + +0.3
2,017,356 - +0.0
2,017,465 + mug NOLOHH_10440 0.24 -0.8
2,017,466 - mug NOLOHH_10440 0.24 -2.7
2,017,467 + mug NOLOHH_10440 0.24 -1.8
2,017,467 + mug NOLOHH_10440 0.24 +0.2
2,017,467 + mug NOLOHH_10440 0.24 -0.5
2,017,467 + mug NOLOHH_10440 0.24 -0.4
2,017,467 + mug NOLOHH_10440 0.24 +1.4
2,017,468 - mug NOLOHH_10440 0.24 +0.1
2,017,638 + mug NOLOHH_10440 0.58 -0.4
2,017,774 + mug NOLOHH_10440 0.85 +0.8

Or see this region's nucleotide sequence