Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nfeF and Ile2_trna are separated by 53 nucleotides Ile2_trna and mug are separated by 124 nucleotides mug and rpoD are separated by 77 nucleotides
NOLOHH_10430: nfeF - NADPH-dependent ferric chelate reductase, at 2,016,326 to 2,017,090
nfeF
NOLOHH_10435: Ile2_trna - tRNA-Ile2(cat), at 2,017,144 to 2,017,219
_trna
NOLOHH_10440: mug - G/U mismatch-specific DNA glycosylase, at 2,017,344 to 2,017,850
mug
NOLOHH_10445: rpoD - RNA polymerase sigma factor RpoD, at 2,017,928 to 2,019,769
rpoD
Position (kb)
2017
2018 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2016.192 kb on + strand at 2016.193 kb on - strand at 2016.193 kb on - strand at 2016.215 kb on - strand at 2016.223 kb on + strand at 2016.223 kb on + strand at 2016.224 kb on - strand at 2016.224 kb on - strand at 2016.224 kb on - strand at 2016.296 kb on + strand at 2016.460 kb on + strand, within nfeF at 2016.460 kb on + strand, within nfeF at 2016.460 kb on + strand, within nfeF at 2016.460 kb on + strand, within nfeF at 2016.461 kb on - strand, within nfeF at 2016.587 kb on + strand, within nfeF at 2016.588 kb on - strand, within nfeF at 2016.589 kb on + strand, within nfeF at 2016.609 kb on + strand, within nfeF at 2016.609 kb on + strand, within nfeF at 2016.609 kb on + strand, within nfeF at 2016.610 kb on - strand, within nfeF at 2016.610 kb on - strand, within nfeF at 2016.610 kb on - strand, within nfeF at 2016.698 kb on - strand, within nfeF at 2016.755 kb on + strand, within nfeF at 2016.755 kb on + strand, within nfeF at 2016.756 kb on - strand, within nfeF at 2016.836 kb on - strand, within nfeF at 2016.898 kb on + strand, within nfeF at 2016.899 kb on - strand, within nfeF at 2016.899 kb on - strand, within nfeF at 2016.974 kb on + strand, within nfeF at 2016.974 kb on + strand, within nfeF at 2016.975 kb on - strand, within nfeF at 2016.975 kb on - strand, within nfeF at 2017.095 kb on + strand at 2017.098 kb on - strand at 2017.099 kb on + strand at 2017.138 kb on + strand at 2017.138 kb on + strand at 2017.139 kb on - strand at 2017.139 kb on - strand at 2017.139 kb on - strand at 2017.139 kb on - strand at 2017.295 kb on + strand at 2017.312 kb on - strand at 2017.312 kb on - strand at 2017.329 kb on + strand at 2017.330 kb on - strand at 2017.330 kb on - strand at 2017.330 kb on - strand at 2017.339 kb on + strand at 2017.339 kb on + strand at 2017.343 kb on + strand at 2017.355 kb on + strand at 2017.356 kb on - strand at 2017.465 kb on + strand, within mug at 2017.466 kb on - strand, within mug at 2017.467 kb on + strand, within mug at 2017.467 kb on + strand, within mug at 2017.467 kb on + strand, within mug at 2017.467 kb on + strand, within mug at 2017.467 kb on + strand, within mug at 2017.468 kb on - strand, within mug at 2017.638 kb on + strand, within mug at 2017.774 kb on + strand, within mug
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,016,192 + +2.0 2,016,193 - -0.8 2,016,193 - +0.5 2,016,215 - -0.2 2,016,223 + +0.1 2,016,223 + +0.9 2,016,224 - +1.0 2,016,224 - +0.1 2,016,224 - +0.7 2,016,296 + +1.1 2,016,460 + nfeF NOLOHH_10430 0.18 -0.7 2,016,460 + nfeF NOLOHH_10430 0.18 -3.0 2,016,460 + nfeF NOLOHH_10430 0.18 -1.3 2,016,460 + nfeF NOLOHH_10430 0.18 +0.4 2,016,461 - nfeF NOLOHH_10430 0.18 -2.7 2,016,587 + nfeF NOLOHH_10430 0.34 -3.4 2,016,588 - nfeF NOLOHH_10430 0.34 -0.1 2,016,589 + nfeF NOLOHH_10430 0.34 -0.6 2,016,609 + nfeF NOLOHH_10430 0.37 -0.3 2,016,609 + nfeF NOLOHH_10430 0.37 -0.1 2,016,609 + nfeF NOLOHH_10430 0.37 +0.5 2,016,610 - nfeF NOLOHH_10430 0.37 -1.6 2,016,610 - nfeF NOLOHH_10430 0.37 +1.4 2,016,610 - nfeF NOLOHH_10430 0.37 +0.7 2,016,698 - nfeF NOLOHH_10430 0.49 +0.3 2,016,755 + nfeF NOLOHH_10430 0.56 +1.0 2,016,755 + nfeF NOLOHH_10430 0.56 +1.2 2,016,756 - nfeF NOLOHH_10430 0.56 +2.9 2,016,836 - nfeF NOLOHH_10430 0.67 +0.9 2,016,898 + nfeF NOLOHH_10430 0.75 +0.4 2,016,899 - nfeF NOLOHH_10430 0.75 -1.2 2,016,899 - nfeF NOLOHH_10430 0.75 +1.4 2,016,974 + nfeF NOLOHH_10430 0.85 +1.0 2,016,974 + nfeF NOLOHH_10430 0.85 +0.8 2,016,975 - nfeF NOLOHH_10430 0.85 +0.8 2,016,975 - nfeF NOLOHH_10430 0.85 +0.9 2,017,095 + +0.8 2,017,098 - +0.2 2,017,099 + +1.5 2,017,138 + -1.0 2,017,138 + +0.7 2,017,139 - +0.1 2,017,139 - -0.2 2,017,139 - +0.9 2,017,139 - -0.1 2,017,295 + +0.1 2,017,312 - -0.2 2,017,312 - -3.3 2,017,329 + -0.7 2,017,330 - +0.7 2,017,330 - +0.8 2,017,330 - +0.2 2,017,339 + -3.2 2,017,339 + -1.6 2,017,343 + -3.1 2,017,355 + +0.3 2,017,356 - +0.0 2,017,465 + mug NOLOHH_10440 0.24 -0.8 2,017,466 - mug NOLOHH_10440 0.24 -2.7 2,017,467 + mug NOLOHH_10440 0.24 -1.8 2,017,467 + mug NOLOHH_10440 0.24 +0.2 2,017,467 + mug NOLOHH_10440 0.24 -0.5 2,017,467 + mug NOLOHH_10440 0.24 -0.4 2,017,467 + mug NOLOHH_10440 0.24 +1.4 2,017,468 - mug NOLOHH_10440 0.24 +0.1 2,017,638 + mug NOLOHH_10440 0.58 -0.4 2,017,774 + mug NOLOHH_10440 0.85 +0.8
Or see this region's nucleotide sequence