Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt asd and yhgO are separated by 73 nucleotides yhgO and glgB are separated by 157 nucleotides
NOLOHH_08665: asd - aspartate-semialdehyde dehydrogenase, at 1,675,015 to 1,676,118
asd
NOLOHH_08670: yhgO - Protein YhgO, at 1,676,192 to 1,676,233
yhgO
NOLOHH_08675: glgB - 1,4-alpha-glucan branching enzyme, at 1,676,391 to 1,678,577
glgB
Position (kb)
1676
1677 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1676.116 kb on + strand at 1676.131 kb on - strand at 1676.165 kb on + strand at 1676.165 kb on + strand at 1676.165 kb on + strand at 1676.166 kb on - strand at 1676.166 kb on - strand at 1676.168 kb on + strand at 1676.168 kb on + strand at 1676.168 kb on + strand at 1676.176 kb on + strand at 1676.176 kb on + strand at 1676.176 kb on + strand at 1676.177 kb on - strand at 1676.177 kb on - strand at 1676.177 kb on - strand at 1676.177 kb on - strand at 1676.208 kb on + strand, within yhgO at 1676.208 kb on + strand, within yhgO at 1676.209 kb on - strand, within yhgO at 1676.212 kb on + strand, within yhgO at 1676.213 kb on - strand, within yhgO at 1676.213 kb on - strand, within yhgO at 1676.245 kb on + strand at 1676.245 kb on + strand at 1676.246 kb on - strand at 1676.246 kb on - strand at 1676.253 kb on + strand at 1676.254 kb on - strand at 1676.265 kb on + strand at 1676.265 kb on + strand at 1676.373 kb on + strand at 1676.374 kb on - strand at 1676.374 kb on - strand at 1676.374 kb on - strand at 1676.409 kb on - strand at 1676.421 kb on - strand at 1676.429 kb on - strand at 1676.474 kb on + strand at 1676.475 kb on - strand at 1676.475 kb on - strand at 1676.618 kb on + strand, within glgB at 1676.725 kb on + strand, within glgB at 1676.739 kb on - strand, within glgB at 1676.765 kb on - strand, within glgB at 1676.780 kb on + strand, within glgB at 1676.780 kb on + strand, within glgB at 1676.780 kb on + strand, within glgB at 1676.780 kb on + strand, within glgB at 1676.780 kb on + strand, within glgB at 1676.780 kb on + strand, within glgB at 1676.781 kb on - strand, within glgB at 1676.783 kb on + strand, within glgB at 1676.783 kb on + strand, within glgB at 1676.783 kb on + strand, within glgB at 1676.784 kb on - strand, within glgB at 1676.802 kb on - strand, within glgB at 1676.858 kb on + strand, within glgB at 1676.859 kb on - strand, within glgB at 1676.859 kb on - strand, within glgB at 1676.863 kb on - strand, within glgB at 1676.897 kb on + strand, within glgB at 1676.953 kb on - strand, within glgB at 1676.953 kb on - strand, within glgB at 1676.955 kb on - strand, within glgB at 1676.955 kb on - strand, within glgB at 1676.958 kb on + strand, within glgB at 1676.958 kb on + strand, within glgB at 1676.959 kb on - strand, within glgB at 1676.959 kb on - strand, within glgB at 1677.048 kb on - strand, within glgB at 1677.048 kb on - strand, within glgB at 1677.073 kb on + strand, within glgB at 1677.073 kb on + strand, within glgB at 1677.126 kb on + strand, within glgB at 1677.127 kb on - strand, within glgB at 1677.127 kb on - strand, within glgB at 1677.213 kb on + strand, within glgB at 1677.214 kb on - strand, within glgB at 1677.218 kb on + strand, within glgB at 1677.218 kb on + strand, within glgB at 1677.219 kb on - strand, within glgB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,676,116 + +0.6 1,676,131 - -1.3 1,676,165 + +0.4 1,676,165 + +0.5 1,676,165 + -0.4 1,676,166 - +0.2 1,676,166 - -3.4 1,676,168 + -0.7 1,676,168 + +1.9 1,676,168 + +0.8 1,676,176 + +0.1 1,676,176 + +1.0 1,676,176 + +0.2 1,676,177 - -0.6 1,676,177 - -0.1 1,676,177 - +0.4 1,676,177 - +0.2 1,676,208 + yhgO NOLOHH_08670 0.38 -3.5 1,676,208 + yhgO NOLOHH_08670 0.38 -1.2 1,676,209 - yhgO NOLOHH_08670 0.40 +1.4 1,676,212 + yhgO NOLOHH_08670 0.48 -3.4 1,676,213 - yhgO NOLOHH_08670 0.50 -1.3 1,676,213 - yhgO NOLOHH_08670 0.50 -0.2 1,676,245 + +1.6 1,676,245 + +0.3 1,676,246 - -0.4 1,676,246 - -1.6 1,676,253 + -3.7 1,676,254 - +0.9 1,676,265 + -0.1 1,676,265 + -0.6 1,676,373 + -1.5 1,676,374 - +0.8 1,676,374 - +1.0 1,676,374 - +0.1 1,676,409 - +0.1 1,676,421 - -0.3 1,676,429 - +0.0 1,676,474 + -0.3 1,676,475 - -0.9 1,676,475 - +0.0 1,676,618 + glgB NOLOHH_08675 0.10 -0.2 1,676,725 + glgB NOLOHH_08675 0.15 +1.4 1,676,739 - glgB NOLOHH_08675 0.16 -0.2 1,676,765 - glgB NOLOHH_08675 0.17 -0.7 1,676,780 + glgB NOLOHH_08675 0.18 +0.6 1,676,780 + glgB NOLOHH_08675 0.18 -0.8 1,676,780 + glgB NOLOHH_08675 0.18 +2.2 1,676,780 + glgB NOLOHH_08675 0.18 +0.6 1,676,780 + glgB NOLOHH_08675 0.18 -0.0 1,676,780 + glgB NOLOHH_08675 0.18 -0.2 1,676,781 - glgB NOLOHH_08675 0.18 +0.5 1,676,783 + glgB NOLOHH_08675 0.18 +1.1 1,676,783 + glgB NOLOHH_08675 0.18 +0.1 1,676,783 + glgB NOLOHH_08675 0.18 -0.2 1,676,784 - glgB NOLOHH_08675 0.18 -0.3 1,676,802 - glgB NOLOHH_08675 0.19 +0.2 1,676,858 + glgB NOLOHH_08675 0.21 -0.6 1,676,859 - glgB NOLOHH_08675 0.21 +0.5 1,676,859 - glgB NOLOHH_08675 0.21 +0.0 1,676,863 - glgB NOLOHH_08675 0.22 +1.0 1,676,897 + glgB NOLOHH_08675 0.23 -1.1 1,676,953 - glgB NOLOHH_08675 0.26 -1.0 1,676,953 - glgB NOLOHH_08675 0.26 -0.6 1,676,955 - glgB NOLOHH_08675 0.26 -0.8 1,676,955 - glgB NOLOHH_08675 0.26 -4.9 1,676,958 + glgB NOLOHH_08675 0.26 +0.4 1,676,958 + glgB NOLOHH_08675 0.26 -1.1 1,676,959 - glgB NOLOHH_08675 0.26 -0.0 1,676,959 - glgB NOLOHH_08675 0.26 -0.7 1,677,048 - glgB NOLOHH_08675 0.30 +0.2 1,677,048 - glgB NOLOHH_08675 0.30 -2.7 1,677,073 + glgB NOLOHH_08675 0.31 +0.1 1,677,073 + glgB NOLOHH_08675 0.31 -1.4 1,677,126 + glgB NOLOHH_08675 0.34 +0.8 1,677,127 - glgB NOLOHH_08675 0.34 +0.9 1,677,127 - glgB NOLOHH_08675 0.34 +0.2 1,677,213 + glgB NOLOHH_08675 0.38 +1.0 1,677,214 - glgB NOLOHH_08675 0.38 -3.6 1,677,218 + glgB NOLOHH_08675 0.38 +0.3 1,677,218 + glgB NOLOHH_08675 0.38 -0.1 1,677,219 - glgB NOLOHH_08675 0.38 +0.6
Or see this region's nucleotide sequence