Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrfD and nrfC overlap by 4 nucleotides nrfC and nrfB overlap by 4 nucleotides nrfB and nrfA are separated by 44 nucleotides
NOLOHH_05055: nrfD - cytochrome c nitrite reductase subunit NrfD, at 927,123 to 928,079
nrfD
NOLOHH_05060: nrfC - cytochrome c nitrite reductase Fe-S protein, at 928,076 to 928,747
nrfC
NOLOHH_05065: nrfB - cytochrome c nitrite reductase pentaheme subunit, at 928,744 to 929,310
nrfB
NOLOHH_05070: nrfA - ammonia-forming nitrite reductase cytochrome c552 subunit, at 929,355 to 930,791
nrfA
Position (kb) 928
929
930 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 927.769 kb on + strand, within nrfD at 927.769 kb on + strand, within nrfD at 927.769 kb on + strand, within nrfD at 927.769 kb on + strand, within nrfD at 927.770 kb on - strand, within nrfD at 927.770 kb on - strand, within nrfD at 927.770 kb on - strand, within nrfD at 927.770 kb on - strand, within nrfD at 927.770 kb on - strand, within nrfD at 927.770 kb on - strand, within nrfD at 927.770 kb on - strand, within nrfD at 927.770 kb on - strand, within nrfD at 927.770 kb on - strand, within nrfD at 927.809 kb on + strand, within nrfD at 927.817 kb on + strand, within nrfD at 927.817 kb on + strand, within nrfD at 927.929 kb on + strand, within nrfD at 927.929 kb on + strand, within nrfD at 927.930 kb on - strand, within nrfD at 927.930 kb on - strand, within nrfD at 927.966 kb on + strand, within nrfD at 927.966 kb on + strand, within nrfD at 928.018 kb on + strand at 928.095 kb on + strand at 928.099 kb on + strand at 928.100 kb on - strand at 928.100 kb on - strand at 928.100 kb on - strand at 928.147 kb on - strand, within nrfC at 928.147 kb on - strand, within nrfC at 928.147 kb on - strand, within nrfC at 928.153 kb on - strand, within nrfC at 928.156 kb on + strand, within nrfC at 928.156 kb on + strand, within nrfC at 928.157 kb on - strand, within nrfC at 928.157 kb on - strand, within nrfC at 928.192 kb on + strand, within nrfC at 928.193 kb on - strand, within nrfC at 928.359 kb on + strand, within nrfC at 928.359 kb on + strand, within nrfC at 928.387 kb on + strand, within nrfC at 928.388 kb on - strand, within nrfC at 928.388 kb on - strand, within nrfC at 928.481 kb on - strand, within nrfC at 928.534 kb on + strand, within nrfC at 928.535 kb on - strand, within nrfC at 928.614 kb on - strand, within nrfC at 928.758 kb on + strand at 928.830 kb on + strand, within nrfB at 928.849 kb on + strand, within nrfB at 928.850 kb on - strand, within nrfB at 928.863 kb on + strand, within nrfB at 928.864 kb on - strand, within nrfB at 928.864 kb on - strand, within nrfB at 928.864 kb on - strand, within nrfB at 928.971 kb on + strand, within nrfB at 928.971 kb on + strand, within nrfB at 928.971 kb on + strand, within nrfB at 928.972 kb on - strand, within nrfB at 928.972 kb on - strand, within nrfB at 928.972 kb on - strand, within nrfB at 929.000 kb on + strand, within nrfB at 929.001 kb on - strand, within nrfB at 929.001 kb on - strand, within nrfB at 929.001 kb on - strand, within nrfB at 929.001 kb on - strand, within nrfB at 929.001 kb on - strand, within nrfB at 929.001 kb on - strand, within nrfB at 929.133 kb on + strand, within nrfB at 929.133 kb on + strand, within nrfB at 929.133 kb on + strand, within nrfB at 929.133 kb on + strand, within nrfB at 929.133 kb on + strand, within nrfB at 929.134 kb on - strand, within nrfB at 929.134 kb on - strand, within nrfB at 929.134 kb on - strand, within nrfB at 929.134 kb on - strand, within nrfB at 929.134 kb on - strand, within nrfB at 929.151 kb on + strand, within nrfB at 929.151 kb on + strand, within nrfB at 929.151 kb on + strand, within nrfB at 929.152 kb on - strand, within nrfB at 929.152 kb on - strand, within nrfB at 929.152 kb on - strand, within nrfB at 929.287 kb on + strand at 929.288 kb on - strand at 929.290 kb on + strand at 929.290 kb on + strand at 929.299 kb on + strand at 929.299 kb on + strand at 929.299 kb on + strand at 929.299 kb on + strand at 929.299 kb on + strand at 929.302 kb on + strand at 929.302 kb on + strand at 929.303 kb on - strand at 929.303 kb on - strand at 929.310 kb on + strand at 929.310 kb on + strand at 929.310 kb on + strand at 929.378 kb on + strand at 929.619 kb on - strand, within nrfA at 929.619 kb on - strand, within nrfA at 929.619 kb on - strand, within nrfA at 929.619 kb on - strand, within nrfA at 929.619 kb on - strand, within nrfA at 929.796 kb on - strand, within nrfA at 929.796 kb on - strand, within nrfA at 929.889 kb on - strand, within nrfA at 929.889 kb on - strand, within nrfA at 929.889 kb on - strand, within nrfA at 929.900 kb on - strand, within nrfA at 929.933 kb on + strand, within nrfA at 929.933 kb on + strand, within nrfA at 929.992 kb on + strand, within nrfA at 929.993 kb on - strand, within nrfA at 929.993 kb on - strand, within nrfA at 929.993 kb on - strand, within nrfA at 929.993 kb on - strand, within nrfA at 930.038 kb on + strand, within nrfA at 930.038 kb on + strand, within nrfA at 930.039 kb on - strand, within nrfA at 930.039 kb on - strand, within nrfA at 930.039 kb on - strand, within nrfA at 930.039 kb on - strand, within nrfA at 930.067 kb on + strand, within nrfA at 930.068 kb on - strand, within nrfA at 930.070 kb on + strand, within nrfA at 930.070 kb on + strand, within nrfA at 930.070 kb on + strand, within nrfA at 930.071 kb on - strand, within nrfA at 930.142 kb on + strand, within nrfA at 930.143 kb on - strand, within nrfA at 930.143 kb on - strand, within nrfA at 930.145 kb on + strand, within nrfA at 930.146 kb on - strand, within nrfA at 930.146 kb on - strand, within nrfA at 930.300 kb on - strand, within nrfA at 930.301 kb on - strand, within nrfA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 927,769 + nrfD NOLOHH_05055 0.68 +1.2 927,769 + nrfD NOLOHH_05055 0.68 -1.7 927,769 + nrfD NOLOHH_05055 0.68 -0.4 927,769 + nrfD NOLOHH_05055 0.68 +1.6 927,770 - nrfD NOLOHH_05055 0.68 -1.1 927,770 - nrfD NOLOHH_05055 0.68 +0.7 927,770 - nrfD NOLOHH_05055 0.68 +0.0 927,770 - nrfD NOLOHH_05055 0.68 -3.0 927,770 - nrfD NOLOHH_05055 0.68 +0.9 927,770 - nrfD NOLOHH_05055 0.68 -1.7 927,770 - nrfD NOLOHH_05055 0.68 -0.3 927,770 - nrfD NOLOHH_05055 0.68 +1.5 927,770 - nrfD NOLOHH_05055 0.68 +1.0 927,809 + nrfD NOLOHH_05055 0.72 +0.4 927,817 + nrfD NOLOHH_05055 0.73 +0.3 927,817 + nrfD NOLOHH_05055 0.73 +0.9 927,929 + nrfD NOLOHH_05055 0.84 -2.8 927,929 + nrfD NOLOHH_05055 0.84 +0.4 927,930 - nrfD NOLOHH_05055 0.84 -1.1 927,930 - nrfD NOLOHH_05055 0.84 +0.9 927,966 + nrfD NOLOHH_05055 0.88 +0.8 927,966 + nrfD NOLOHH_05055 0.88 -0.3 928,018 + +0.9 928,095 + +0.7 928,099 + -1.0 928,100 - +0.4 928,100 - -0.3 928,100 - -0.4 928,147 - nrfC NOLOHH_05060 0.11 +0.5 928,147 - nrfC NOLOHH_05060 0.11 +1.1 928,147 - nrfC NOLOHH_05060 0.11 +1.4 928,153 - nrfC NOLOHH_05060 0.11 -0.8 928,156 + nrfC NOLOHH_05060 0.12 -0.2 928,156 + nrfC NOLOHH_05060 0.12 +0.4 928,157 - nrfC NOLOHH_05060 0.12 +1.8 928,157 - nrfC NOLOHH_05060 0.12 +0.3 928,192 + nrfC NOLOHH_05060 0.17 -0.5 928,193 - nrfC NOLOHH_05060 0.17 -0.4 928,359 + nrfC NOLOHH_05060 0.42 -0.3 928,359 + nrfC NOLOHH_05060 0.42 +0.8 928,387 + nrfC NOLOHH_05060 0.46 +1.5 928,388 - nrfC NOLOHH_05060 0.46 +0.8 928,388 - nrfC NOLOHH_05060 0.46 -0.0 928,481 - nrfC NOLOHH_05060 0.60 -2.5 928,534 + nrfC NOLOHH_05060 0.68 +0.7 928,535 - nrfC NOLOHH_05060 0.68 +1.0 928,614 - nrfC NOLOHH_05060 0.80 +0.4 928,758 + -0.5 928,830 + nrfB NOLOHH_05065 0.15 +0.2 928,849 + nrfB NOLOHH_05065 0.19 +1.0 928,850 - nrfB NOLOHH_05065 0.19 +0.3 928,863 + nrfB NOLOHH_05065 0.21 -1.1 928,864 - nrfB NOLOHH_05065 0.21 +0.9 928,864 - nrfB NOLOHH_05065 0.21 -0.4 928,864 - nrfB NOLOHH_05065 0.21 -1.7 928,971 + nrfB NOLOHH_05065 0.40 -0.7 928,971 + nrfB NOLOHH_05065 0.40 +0.9 928,971 + nrfB NOLOHH_05065 0.40 +1.2 928,972 - nrfB NOLOHH_05065 0.40 +1.0 928,972 - nrfB NOLOHH_05065 0.40 -1.1 928,972 - nrfB NOLOHH_05065 0.40 -0.2 929,000 + nrfB NOLOHH_05065 0.45 -0.4 929,001 - nrfB NOLOHH_05065 0.45 -0.1 929,001 - nrfB NOLOHH_05065 0.45 +1.4 929,001 - nrfB NOLOHH_05065 0.45 +0.0 929,001 - nrfB NOLOHH_05065 0.45 +0.9 929,001 - nrfB NOLOHH_05065 0.45 +0.3 929,001 - nrfB NOLOHH_05065 0.45 -3.1 929,133 + nrfB NOLOHH_05065 0.69 -1.1 929,133 + nrfB NOLOHH_05065 0.69 -0.0 929,133 + nrfB NOLOHH_05065 0.69 +0.0 929,133 + nrfB NOLOHH_05065 0.69 -2.8 929,133 + nrfB NOLOHH_05065 0.69 -0.4 929,134 - nrfB NOLOHH_05065 0.69 -2.6 929,134 - nrfB NOLOHH_05065 0.69 -0.2 929,134 - nrfB NOLOHH_05065 0.69 +1.2 929,134 - nrfB NOLOHH_05065 0.69 -0.0 929,134 - nrfB NOLOHH_05065 0.69 +0.3 929,151 + nrfB NOLOHH_05065 0.72 -2.0 929,151 + nrfB NOLOHH_05065 0.72 +1.4 929,151 + nrfB NOLOHH_05065 0.72 -0.9 929,152 - nrfB NOLOHH_05065 0.72 -0.4 929,152 - nrfB NOLOHH_05065 0.72 -0.4 929,152 - nrfB NOLOHH_05065 0.72 +0.3 929,287 + +0.5 929,288 - -3.5 929,290 + +0.4 929,290 + -0.8 929,299 + -1.2 929,299 + -1.1 929,299 + +0.0 929,299 + +0.4 929,299 + +1.0 929,302 + -1.0 929,302 + +0.1 929,303 - -0.2 929,303 - -0.2 929,310 + +2.0 929,310 + +1.2 929,310 + +2.2 929,378 + +1.8 929,619 - nrfA NOLOHH_05070 0.18 +0.0 929,619 - nrfA NOLOHH_05070 0.18 +1.1 929,619 - nrfA NOLOHH_05070 0.18 +0.3 929,619 - nrfA NOLOHH_05070 0.18 -0.1 929,619 - nrfA NOLOHH_05070 0.18 +0.3 929,796 - nrfA NOLOHH_05070 0.31 -0.4 929,796 - nrfA NOLOHH_05070 0.31 -0.5 929,889 - nrfA NOLOHH_05070 0.37 -3.7 929,889 - nrfA NOLOHH_05070 0.37 +0.0 929,889 - nrfA NOLOHH_05070 0.37 +0.7 929,900 - nrfA NOLOHH_05070 0.38 +0.3 929,933 + nrfA NOLOHH_05070 0.40 +1.0 929,933 + nrfA NOLOHH_05070 0.40 -0.0 929,992 + nrfA NOLOHH_05070 0.44 -0.8 929,993 - nrfA NOLOHH_05070 0.44 +0.2 929,993 - nrfA NOLOHH_05070 0.44 +0.4 929,993 - nrfA NOLOHH_05070 0.44 +0.3 929,993 - nrfA NOLOHH_05070 0.44 -3.5 930,038 + nrfA NOLOHH_05070 0.48 -0.6 930,038 + nrfA NOLOHH_05070 0.48 +1.3 930,039 - nrfA NOLOHH_05070 0.48 +1.0 930,039 - nrfA NOLOHH_05070 0.48 +0.2 930,039 - nrfA NOLOHH_05070 0.48 +0.5 930,039 - nrfA NOLOHH_05070 0.48 -1.5 930,067 + nrfA NOLOHH_05070 0.50 +0.3 930,068 - nrfA NOLOHH_05070 0.50 +0.7 930,070 + nrfA NOLOHH_05070 0.50 +2.0 930,070 + nrfA NOLOHH_05070 0.50 +1.4 930,070 + nrfA NOLOHH_05070 0.50 +1.7 930,071 - nrfA NOLOHH_05070 0.50 -0.6 930,142 + nrfA NOLOHH_05070 0.55 +0.6 930,143 - nrfA NOLOHH_05070 0.55 -3.1 930,143 - nrfA NOLOHH_05070 0.55 -0.4 930,145 + nrfA NOLOHH_05070 0.55 -0.4 930,146 - nrfA NOLOHH_05070 0.55 +1.0 930,146 - nrfA NOLOHH_05070 0.55 +0.5 930,300 - nrfA NOLOHH_05070 0.66 -0.3 930,301 - nrfA NOLOHH_05070 0.66 -0.5
Or see this region's nucleotide sequence