Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hpaD and hpaF are separated by 9 nucleotides hpaF and hpaH are separated by 67 nucleotides hpaH and hpaI are separated by 10 nucleotides hpaI and hpaX are separated by 174 nucleotides
NOLOHH_03725: hpaD - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 663,081 to 663,932
hpaD
NOLOHH_03730: hpaF - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 663,942 to 664,322
hpaF
NOLOHH_03735: hpaH - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 664,390 to 665,193
hpaH
NOLOHH_03740: hpaI - 4-hydroxy-2-oxoheptanedioate aldolase, at 665,204 to 665,992
hpaI
NOLOHH_03745: hpaX - 4-hydroxyphenylacetate permease, at 666,167 to 667,543
hpaX
Position (kb) 664
665
666 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 663.591 kb on + strand, within hpaD at 663.591 kb on + strand, within hpaD at 663.641 kb on + strand, within hpaD at 663.642 kb on - strand, within hpaD at 663.678 kb on + strand, within hpaD at 663.679 kb on - strand, within hpaD at 663.758 kb on + strand, within hpaD at 663.758 kb on + strand, within hpaD at 663.758 kb on + strand, within hpaD at 663.759 kb on - strand, within hpaD at 663.759 kb on - strand, within hpaD at 663.759 kb on - strand, within hpaD at 663.772 kb on - strand, within hpaD at 663.781 kb on - strand, within hpaD at 663.781 kb on - strand, within hpaD at 663.786 kb on + strand, within hpaD at 663.800 kb on + strand, within hpaD at 663.801 kb on - strand, within hpaD at 663.846 kb on + strand, within hpaD at 663.846 kb on + strand, within hpaD at 663.847 kb on - strand at 663.847 kb on - strand at 663.847 kb on - strand at 663.847 kb on - strand at 663.847 kb on - strand at 663.867 kb on - strand at 663.965 kb on + strand at 663.966 kb on - strand at 663.975 kb on - strand at 663.994 kb on + strand, within hpaF at 664.016 kb on + strand, within hpaF at 664.016 kb on + strand, within hpaF at 664.060 kb on + strand, within hpaF at 664.060 kb on + strand, within hpaF at 664.060 kb on + strand, within hpaF at 664.060 kb on + strand, within hpaF at 664.061 kb on - strand, within hpaF at 664.061 kb on - strand, within hpaF at 664.061 kb on - strand, within hpaF at 664.064 kb on + strand, within hpaF at 664.064 kb on + strand, within hpaF at 664.086 kb on - strand, within hpaF at 664.117 kb on - strand, within hpaF at 664.130 kb on + strand, within hpaF at 664.130 kb on + strand, within hpaF at 664.130 kb on + strand, within hpaF at 664.131 kb on - strand, within hpaF at 664.316 kb on + strand at 664.389 kb on + strand at 664.389 kb on + strand at 664.390 kb on - strand at 664.390 kb on - strand at 664.671 kb on + strand, within hpaH at 664.772 kb on - strand, within hpaH at 664.786 kb on + strand, within hpaH at 664.786 kb on + strand, within hpaH at 664.786 kb on + strand, within hpaH at 664.786 kb on + strand, within hpaH at 664.787 kb on - strand, within hpaH at 664.923 kb on + strand, within hpaH at 664.924 kb on - strand, within hpaH at 664.924 kb on - strand, within hpaH at 664.943 kb on + strand, within hpaH at 664.943 kb on + strand, within hpaH at 664.944 kb on - strand, within hpaH at 664.944 kb on - strand, within hpaH at 664.944 kb on - strand, within hpaH at 664.944 kb on - strand, within hpaH at 664.967 kb on - strand, within hpaH at 665.062 kb on + strand, within hpaH at 665.062 kb on + strand, within hpaH at 665.063 kb on - strand, within hpaH at 665.063 kb on - strand, within hpaH at 665.069 kb on + strand, within hpaH at 665.069 kb on + strand, within hpaH at 665.069 kb on + strand, within hpaH at 665.069 kb on + strand, within hpaH at 665.069 kb on + strand, within hpaH at 665.070 kb on - strand, within hpaH at 665.070 kb on - strand, within hpaH at 665.070 kb on - strand, within hpaH at 665.070 kb on - strand, within hpaH at 665.070 kb on - strand, within hpaH at 665.175 kb on + strand at 665.279 kb on + strand at 665.280 kb on - strand at 665.410 kb on - strand, within hpaI at 665.410 kb on - strand, within hpaI at 665.747 kb on + strand, within hpaI at 665.776 kb on - strand, within hpaI at 665.868 kb on - strand, within hpaI at 665.987 kb on + strand at 665.987 kb on + strand at 665.988 kb on - strand at 665.988 kb on - strand at 665.988 kb on - strand at 665.990 kb on + strand at 665.991 kb on - strand at 666.011 kb on + strand at 666.012 kb on - strand at 666.020 kb on + strand at 666.020 kb on + strand at 666.020 kb on + strand at 666.124 kb on - strand at 666.124 kb on - strand at 666.124 kb on - strand at 666.136 kb on - strand at 666.141 kb on - strand at 666.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 663,591 + hpaD NOLOHH_03725 0.60 -0.2 663,591 + hpaD NOLOHH_03725 0.60 +0.1 663,641 + hpaD NOLOHH_03725 0.66 -3.6 663,642 - hpaD NOLOHH_03725 0.66 +0.9 663,678 + hpaD NOLOHH_03725 0.70 -4.2 663,679 - hpaD NOLOHH_03725 0.70 -0.2 663,758 + hpaD NOLOHH_03725 0.79 +0.4 663,758 + hpaD NOLOHH_03725 0.79 +0.8 663,758 + hpaD NOLOHH_03725 0.79 -1.0 663,759 - hpaD NOLOHH_03725 0.80 -4.2 663,759 - hpaD NOLOHH_03725 0.80 +1.4 663,759 - hpaD NOLOHH_03725 0.80 +0.1 663,772 - hpaD NOLOHH_03725 0.81 -0.3 663,781 - hpaD NOLOHH_03725 0.82 -1.5 663,781 - hpaD NOLOHH_03725 0.82 -0.2 663,786 + hpaD NOLOHH_03725 0.83 +0.3 663,800 + hpaD NOLOHH_03725 0.84 +1.0 663,801 - hpaD NOLOHH_03725 0.85 -2.4 663,846 + hpaD NOLOHH_03725 0.90 -0.2 663,846 + hpaD NOLOHH_03725 0.90 -0.4 663,847 - +0.7 663,847 - +0.4 663,847 - +0.2 663,847 - +0.1 663,847 - +0.7 663,867 - -2.1 663,965 + -0.1 663,966 - +0.6 663,975 - +0.8 663,994 + hpaF NOLOHH_03730 0.14 +1.2 664,016 + hpaF NOLOHH_03730 0.19 -0.1 664,016 + hpaF NOLOHH_03730 0.19 +0.5 664,060 + hpaF NOLOHH_03730 0.31 -1.8 664,060 + hpaF NOLOHH_03730 0.31 +0.7 664,060 + hpaF NOLOHH_03730 0.31 -0.3 664,060 + hpaF NOLOHH_03730 0.31 +0.6 664,061 - hpaF NOLOHH_03730 0.31 -0.9 664,061 - hpaF NOLOHH_03730 0.31 -3.4 664,061 - hpaF NOLOHH_03730 0.31 +0.2 664,064 + hpaF NOLOHH_03730 0.32 -0.0 664,064 + hpaF NOLOHH_03730 0.32 -4.4 664,086 - hpaF NOLOHH_03730 0.38 -0.5 664,117 - hpaF NOLOHH_03730 0.46 +0.9 664,130 + hpaF NOLOHH_03730 0.49 +0.1 664,130 + hpaF NOLOHH_03730 0.49 +0.9 664,130 + hpaF NOLOHH_03730 0.49 -0.0 664,131 - hpaF NOLOHH_03730 0.50 -0.8 664,316 + +0.0 664,389 + -1.2 664,389 + -0.4 664,390 - +0.3 664,390 - +0.6 664,671 + hpaH NOLOHH_03735 0.35 -0.7 664,772 - hpaH NOLOHH_03735 0.48 +1.5 664,786 + hpaH NOLOHH_03735 0.49 -3.0 664,786 + hpaH NOLOHH_03735 0.49 +1.9 664,786 + hpaH NOLOHH_03735 0.49 -2.1 664,786 + hpaH NOLOHH_03735 0.49 +0.2 664,787 - hpaH NOLOHH_03735 0.49 -3.4 664,923 + hpaH NOLOHH_03735 0.66 -0.1 664,924 - hpaH NOLOHH_03735 0.66 -0.3 664,924 - hpaH NOLOHH_03735 0.66 +1.3 664,943 + hpaH NOLOHH_03735 0.69 +0.7 664,943 + hpaH NOLOHH_03735 0.69 +0.1 664,944 - hpaH NOLOHH_03735 0.69 -3.3 664,944 - hpaH NOLOHH_03735 0.69 +0.9 664,944 - hpaH NOLOHH_03735 0.69 -0.0 664,944 - hpaH NOLOHH_03735 0.69 -1.6 664,967 - hpaH NOLOHH_03735 0.72 +1.7 665,062 + hpaH NOLOHH_03735 0.84 +0.8 665,062 + hpaH NOLOHH_03735 0.84 -2.0 665,063 - hpaH NOLOHH_03735 0.84 -0.3 665,063 - hpaH NOLOHH_03735 0.84 +0.9 665,069 + hpaH NOLOHH_03735 0.84 -3.6 665,069 + hpaH NOLOHH_03735 0.84 +0.7 665,069 + hpaH NOLOHH_03735 0.84 +0.3 665,069 + hpaH NOLOHH_03735 0.84 -0.9 665,069 + hpaH NOLOHH_03735 0.84 +0.2 665,070 - hpaH NOLOHH_03735 0.85 +0.7 665,070 - hpaH NOLOHH_03735 0.85 +0.8 665,070 - hpaH NOLOHH_03735 0.85 +0.9 665,070 - hpaH NOLOHH_03735 0.85 -0.2 665,070 - hpaH NOLOHH_03735 0.85 +1.7 665,175 + +1.1 665,279 + +0.0 665,280 - +0.6 665,410 - hpaI NOLOHH_03740 0.26 -2.9 665,410 - hpaI NOLOHH_03740 0.26 -2.7 665,747 + hpaI NOLOHH_03740 0.69 +0.5 665,776 - hpaI NOLOHH_03740 0.72 -2.6 665,868 - hpaI NOLOHH_03740 0.84 +1.0 665,987 + -0.3 665,987 + -0.6 665,988 - +0.8 665,988 - -1.6 665,988 - +0.1 665,990 + +0.0 665,991 - +0.7 666,011 + +0.9 666,012 - +0.6 666,020 + -2.6 666,020 + -2.0 666,020 + -0.2 666,124 - -3.9 666,124 - +0.6 666,124 - +0.5 666,136 - -0.3 666,141 - -3.0 666,190 - +0.7
Or see this region's nucleotide sequence