Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NOLOHH_00300 and virB9 overlap by 11 nucleotides virB9 and NOLOHH_00310 overlap by 4 nucleotides NOLOHH_00310 and NOLOHH_00315 overlap by 11 nucleotides NOLOHH_00315 and NOLOHH_00320 are separated by 2 nucleotides NOLOHH_00320 and NOLOHH_00325 are separated by 14 nucleotides
NOLOHH_00300: NOLOHH_00300 - conjugal transfer protein TrbI, at 9,087 to 10,370
_00300
NOLOHH_00305: virB9 - P-type conjugative transfer protein VirB9, at 10,360 to 11,268
virB9
NOLOHH_00310: NOLOHH_00310 - VirB8 domain-containing protein, at 11,265 to 11,954
_00310
NOLOHH_00315: NOLOHH_00315 - Cnjugal protein Pilx7, at 11,944 to 12,084
_00315
NOLOHH_00320: NOLOHH_00320 - hypothetical protein, at 12,087 to 12,302
_00320
NOLOHH_00325: NOLOHH_00325 - conjugal transfer protein TrbL, at 12,317 to 13,375
_00325
Position (kb)
11
12 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 10.290 kb on - strand at 10.447 kb on - strand at 10.457 kb on - strand, within virB9 at 10.501 kb on - strand, within virB9 at 10.551 kb on + strand, within virB9 at 10.551 kb on + strand, within virB9 at 10.620 kb on - strand, within virB9 at 10.662 kb on + strand, within virB9 at 10.662 kb on + strand, within virB9 at 10.662 kb on + strand, within virB9 at 10.737 kb on + strand, within virB9 at 10.826 kb on + strand, within virB9 at 10.838 kb on + strand, within virB9 at 10.838 kb on + strand, within virB9 at 10.839 kb on - strand, within virB9 at 10.839 kb on - strand, within virB9 at 10.849 kb on - strand, within virB9 at 10.880 kb on - strand, within virB9 at 10.893 kb on - strand, within virB9 at 10.893 kb on - strand, within virB9 at 10.945 kb on - strand, within virB9 at 10.972 kb on - strand, within virB9 at 10.972 kb on - strand, within virB9 at 11.019 kb on - strand, within virB9 at 11.158 kb on - strand, within virB9 at 11.159 kb on + strand, within virB9 at 11.160 kb on - strand, within virB9 at 11.160 kb on - strand, within virB9 at 11.215 kb on - strand at 11.219 kb on - strand at 11.257 kb on + strand at 11.262 kb on + strand at 11.379 kb on - strand, within NOLOHH_00310 at 11.379 kb on - strand, within NOLOHH_00310 at 11.381 kb on + strand, within NOLOHH_00310 at 11.556 kb on - strand, within NOLOHH_00310 at 11.586 kb on - strand, within NOLOHH_00310 at 11.683 kb on - strand, within NOLOHH_00310 at 11.710 kb on - strand, within NOLOHH_00310 at 11.712 kb on - strand, within NOLOHH_00310 at 11.900 kb on + strand at 11.971 kb on - strand, within NOLOHH_00315 at 12.056 kb on - strand, within NOLOHH_00315 at 12.110 kb on + strand, within NOLOHH_00320 at 12.111 kb on - strand, within NOLOHH_00320 at 12.279 kb on + strand, within NOLOHH_00320 at 12.338 kb on + strand at 12.343 kb on + strand at 12.428 kb on + strand, within NOLOHH_00325 at 12.429 kb on - strand, within NOLOHH_00325 at 12.446 kb on - strand, within NOLOHH_00325 at 12.524 kb on - strand, within NOLOHH_00325 at 12.579 kb on - strand, within NOLOHH_00325 at 12.579 kb on - strand, within NOLOHH_00325 at 12.579 kb on - strand, within NOLOHH_00325 at 12.638 kb on + strand, within NOLOHH_00325 at 12.654 kb on - strand, within NOLOHH_00325 at 12.655 kb on + strand, within NOLOHH_00325 at 12.656 kb on - strand, within NOLOHH_00325 at 12.656 kb on - strand, within NOLOHH_00325 at 12.658 kb on - strand, within NOLOHH_00325 at 12.660 kb on - strand, within NOLOHH_00325
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 10,290 - -0.6 10,447 - -0.5 10,457 - virB9 NOLOHH_00305 0.11 +0.3 10,501 - virB9 NOLOHH_00305 0.16 -0.0 10,551 + virB9 NOLOHH_00305 0.21 -2.3 10,551 + virB9 NOLOHH_00305 0.21 +0.8 10,620 - virB9 NOLOHH_00305 0.29 +0.6 10,662 + virB9 NOLOHH_00305 0.33 +1.2 10,662 + virB9 NOLOHH_00305 0.33 +0.2 10,662 + virB9 NOLOHH_00305 0.33 +0.5 10,737 + virB9 NOLOHH_00305 0.41 +0.1 10,826 + virB9 NOLOHH_00305 0.51 -0.1 10,838 + virB9 NOLOHH_00305 0.53 +0.2 10,838 + virB9 NOLOHH_00305 0.53 -0.2 10,839 - virB9 NOLOHH_00305 0.53 -0.0 10,839 - virB9 NOLOHH_00305 0.53 +0.4 10,849 - virB9 NOLOHH_00305 0.54 -0.0 10,880 - virB9 NOLOHH_00305 0.57 +0.2 10,893 - virB9 NOLOHH_00305 0.59 -1.4 10,893 - virB9 NOLOHH_00305 0.59 +0.6 10,945 - virB9 NOLOHH_00305 0.64 -0.9 10,972 - virB9 NOLOHH_00305 0.67 -1.4 10,972 - virB9 NOLOHH_00305 0.67 +1.2 11,019 - virB9 NOLOHH_00305 0.72 +0.2 11,158 - virB9 NOLOHH_00305 0.88 -0.5 11,159 + virB9 NOLOHH_00305 0.88 -0.0 11,160 - virB9 NOLOHH_00305 0.88 +0.8 11,160 - virB9 NOLOHH_00305 0.88 -0.3 11,215 - +0.3 11,219 - +0.2 11,257 + -0.5 11,262 + -0.2 11,379 - NOLOHH_00310 0.17 -0.1 11,379 - NOLOHH_00310 0.17 -0.8 11,381 + NOLOHH_00310 0.17 -0.4 11,556 - NOLOHH_00310 0.42 +1.1 11,586 - NOLOHH_00310 0.47 -0.6 11,683 - NOLOHH_00310 0.61 -0.0 11,710 - NOLOHH_00310 0.64 +1.0 11,712 - NOLOHH_00310 0.65 +0.4 11,900 + +0.4 11,971 - NOLOHH_00315 0.19 +0.5 12,056 - NOLOHH_00315 0.79 +0.3 12,110 + NOLOHH_00320 0.11 +0.1 12,111 - NOLOHH_00320 0.11 +0.3 12,279 + NOLOHH_00320 0.89 -0.7 12,338 + -0.7 12,343 + +0.3 12,428 + NOLOHH_00325 0.10 +0.5 12,429 - NOLOHH_00325 0.11 -0.4 12,446 - NOLOHH_00325 0.12 -1.4 12,524 - NOLOHH_00325 0.20 -0.4 12,579 - NOLOHH_00325 0.25 +0.3 12,579 - NOLOHH_00325 0.25 +0.2 12,579 - NOLOHH_00325 0.25 -1.2 12,638 + NOLOHH_00325 0.30 +0.5 12,654 - NOLOHH_00325 0.32 +0.6 12,655 + NOLOHH_00325 0.32 -0.8 12,656 - NOLOHH_00325 0.32 +0.0 12,656 - NOLOHH_00325 0.32 +0.2 12,658 - NOLOHH_00325 0.32 +0.3 12,660 - NOLOHH_00325 0.32 +1.8
Or see this region's nucleotide sequence