Experiment: Columbia 1 x (C) complex media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1181_RS03215 and HMPREF1181_RS03220 are separated by 22 nucleotides HMPREF1181_RS03220 and HMPREF1181_RS03225 are separated by 75 nucleotides HMPREF1181_RS03225 and HMPREF1181_RS03230 are separated by 37 nucleotides
HMPREF1181_RS03215: HMPREF1181_RS03215 - UDP-2,3-diacylglucosamine diphosphatase, at 824,496 to 825,260
_RS03215
HMPREF1181_RS03220: HMPREF1181_RS03220 - DUF59 domain-containing protein, at 825,283 to 825,594
_RS03220
HMPREF1181_RS03225: HMPREF1181_RS03225 - DNA repair protein RadC, at 825,670 to 826,356
_RS03225
HMPREF1181_RS03230: HMPREF1181_RS03230 - glycosyltransferase, at 826,394 to 827,383
_RS03230
Position (kb)
825
826
827 Strain fitness (log2 ratio)
-1
0
1 at 825.491 kb on - strand, within HMPREF1181_RS03220 at 825.498 kb on - strand, within HMPREF1181_RS03220 at 825.648 kb on + strand at 825.701 kb on + strand at 825.721 kb on - strand at 825.721 kb on - strand at 825.926 kb on - strand, within HMPREF1181_RS03225 at 825.979 kb on + strand, within HMPREF1181_RS03225 at 826.017 kb on - strand, within HMPREF1181_RS03225 at 826.385 kb on + strand at 826.386 kb on - strand at 826.386 kb on - strand at 826.395 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Columbia 1 x (C) complex media remove 825,491 - HMPREF1181_RS03220 0.67 -0.6 825,498 - HMPREF1181_RS03220 0.69 -0.7 825,648 + -0.5 825,701 + +0.4 825,721 - -0.5 825,721 - -1.0 825,926 - HMPREF1181_RS03225 0.37 -0.8 825,979 + HMPREF1181_RS03225 0.45 -0.0 826,017 - HMPREF1181_RS03225 0.51 -1.1 826,385 + +0.5 826,386 - +0.3 826,386 - -0.2 826,395 + -0.1
Or see this region's nucleotide sequence