Experiment: BHI 1 x (C) complex media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1181_RS14065 and HMPREF1181_RS18165 are separated by 191 nucleotides HMPREF1181_RS18165 and HMPREF1181_RS14070 are separated by 128 nucleotides HMPREF1181_RS14070 and HMPREF1181_RS14075 are separated by 124 nucleotides HMPREF1181_RS14075 and HMPREF1181_RS14080 are separated by 178 nucleotides
HMPREF1181_RS14065: HMPREF1181_RS14065 - glutamine synthetase, at 336,057 to 337,559
_RS14065
HMPREF1181_RS18165: HMPREF1181_RS18165 - hypothetical protein, at 337,751 to 337,909
_RS18165
HMPREF1181_RS14070: HMPREF1181_RS14070 - histidine--tRNA ligase, at 338,038 to 339,399
_RS14070
HMPREF1181_RS14075: HMPREF1181_RS14075 - zinc metallopeptidase, at 339,524 to 340,219
_RS14075
HMPREF1181_RS14080: HMPREF1181_RS14080 - alpha-L-fucosidase, at 340,398 to 341,702
_RS14080
Position (kb) 338
339
340 Strain fitness (log2 ratio) -2
-1
0
1 at 337.093 kb on + strand, within HMPREF1181_RS14065 at 337.096 kb on + strand, within HMPREF1181_RS14065 at 337.143 kb on - strand, within HMPREF1181_RS14065 at 337.236 kb on - strand, within HMPREF1181_RS14065 at 337.260 kb on - strand, within HMPREF1181_RS14065 at 337.260 kb on - strand, within HMPREF1181_RS14065 at 337.284 kb on + strand, within HMPREF1181_RS14065 at 337.284 kb on + strand, within HMPREF1181_RS14065 at 337.284 kb on + strand, within HMPREF1181_RS14065 at 337.285 kb on - strand, within HMPREF1181_RS14065 at 337.288 kb on + strand, within HMPREF1181_RS14065 at 337.291 kb on - strand, within HMPREF1181_RS14065 at 337.355 kb on + strand, within HMPREF1181_RS14065 at 337.430 kb on - strand at 337.464 kb on + strand at 337.465 kb on - strand at 337.611 kb on - strand at 337.637 kb on + strand at 337.640 kb on - strand at 337.696 kb on + strand at 337.700 kb on + strand at 337.700 kb on + strand at 337.701 kb on - strand at 337.701 kb on - strand at 337.769 kb on + strand, within HMPREF1181_RS18165 at 337.776 kb on + strand, within HMPREF1181_RS18165 at 337.824 kb on + strand, within HMPREF1181_RS18165 at 337.824 kb on + strand, within HMPREF1181_RS18165 at 337.957 kb on - strand at 337.957 kb on - strand at 337.984 kb on + strand at 338.025 kb on - strand at 339.438 kb on - strand at 339.525 kb on + strand at 339.526 kb on - strand at 339.546 kb on - strand at 339.551 kb on + strand at 339.551 kb on + strand at 339.556 kb on - strand at 339.558 kb on + strand at 339.598 kb on - strand, within HMPREF1181_RS14075 at 339.602 kb on - strand, within HMPREF1181_RS14075 at 339.602 kb on - strand, within HMPREF1181_RS14075 at 339.602 kb on - strand, within HMPREF1181_RS14075 at 339.604 kb on - strand, within HMPREF1181_RS14075 at 339.638 kb on - strand, within HMPREF1181_RS14075 at 339.677 kb on + strand, within HMPREF1181_RS14075 at 339.678 kb on - strand, within HMPREF1181_RS14075 at 339.736 kb on + strand, within HMPREF1181_RS14075 at 339.736 kb on + strand, within HMPREF1181_RS14075 at 339.813 kb on - strand, within HMPREF1181_RS14075 at 339.955 kb on + strand, within HMPREF1181_RS14075 at 339.956 kb on - strand, within HMPREF1181_RS14075 at 339.999 kb on + strand, within HMPREF1181_RS14075 at 340.120 kb on - strand, within HMPREF1181_RS14075 at 340.243 kb on + strand at 340.244 kb on - strand at 340.252 kb on - strand at 340.378 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHI 1 x (C) complex media remove 337,093 + HMPREF1181_RS14065 0.69 -0.2 337,096 + HMPREF1181_RS14065 0.69 -0.6 337,143 - HMPREF1181_RS14065 0.72 +0.6 337,236 - HMPREF1181_RS14065 0.78 -0.5 337,260 - HMPREF1181_RS14065 0.80 +0.3 337,260 - HMPREF1181_RS14065 0.80 +0.5 337,284 + HMPREF1181_RS14065 0.82 -0.1 337,284 + HMPREF1181_RS14065 0.82 -0.4 337,284 + HMPREF1181_RS14065 0.82 +0.2 337,285 - HMPREF1181_RS14065 0.82 +0.2 337,288 + HMPREF1181_RS14065 0.82 -0.5 337,291 - HMPREF1181_RS14065 0.82 -0.1 337,355 + HMPREF1181_RS14065 0.86 +0.2 337,430 - -0.4 337,464 + +0.5 337,465 - -0.3 337,611 - +0.3 337,637 + -0.3 337,640 - +0.2 337,696 + -0.2 337,700 + +0.0 337,700 + -0.6 337,701 - +0.0 337,701 - +0.3 337,769 + HMPREF1181_RS18165 0.11 +0.1 337,776 + HMPREF1181_RS18165 0.16 -2.1 337,824 + HMPREF1181_RS18165 0.46 -0.1 337,824 + HMPREF1181_RS18165 0.46 -1.0 337,957 - -0.2 337,957 - -1.1 337,984 + -1.6 338,025 - +0.2 339,438 - +0.6 339,525 + -1.1 339,526 - +0.4 339,546 - -1.1 339,551 + -2.1 339,551 + -0.2 339,556 - -0.5 339,558 + -1.0 339,598 - HMPREF1181_RS14075 0.11 +0.3 339,602 - HMPREF1181_RS14075 0.11 -1.5 339,602 - HMPREF1181_RS14075 0.11 -1.1 339,602 - HMPREF1181_RS14075 0.11 +0.9 339,604 - HMPREF1181_RS14075 0.11 +0.1 339,638 - HMPREF1181_RS14075 0.16 +0.2 339,677 + HMPREF1181_RS14075 0.22 -0.1 339,678 - HMPREF1181_RS14075 0.22 -0.2 339,736 + HMPREF1181_RS14075 0.30 -0.2 339,736 + HMPREF1181_RS14075 0.30 +1.1 339,813 - HMPREF1181_RS14075 0.42 -1.3 339,955 + HMPREF1181_RS14075 0.62 -0.5 339,956 - HMPREF1181_RS14075 0.62 -1.2 339,999 + HMPREF1181_RS14075 0.68 -0.6 340,120 - HMPREF1181_RS14075 0.86 -0.2 340,243 + -0.1 340,244 - -0.4 340,252 - +0.1 340,378 - +0.1
Or see this region's nucleotide sequence