Experiment: Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt coaBC and dut overlap by 23 nucleotides dut and slmA are separated by 117 nucleotides slmA and pyrE are separated by 30 nucleotides pyrE and rph are separated by 54 nucleotides
LU632_RS22000: coaBC - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 4,201,220 to 4,202,434
coaBC
LU632_RS22005: dut - dUTP diphosphatase, at 4,202,412 to 4,202,870
dut
LU632_RS22010: slmA - nucleoid occlusion factor SlmA, at 4,202,988 to 4,203,584
slmA
LU632_RS22015: pyrE - orotate phosphoribosyltransferase, at 4,203,615 to 4,204,256
pyrE
LU632_RS22020: rph - ribonuclease PH, at 4,204,311 to 4,205,027
rph
Position (kb) 4202
4203
4204 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4202.868 kb on + strand at 4202.904 kb on + strand at 4202.963 kb on + strand at 4202.967 kb on - strand at 4203.183 kb on - strand, within slmA at 4203.221 kb on - strand, within slmA at 4203.309 kb on - strand, within slmA at 4203.371 kb on - strand, within slmA at 4203.896 kb on + strand, within pyrE at 4203.897 kb on - strand, within pyrE at 4203.897 kb on - strand, within pyrE at 4203.899 kb on + strand, within pyrE at 4203.899 kb on + strand, within pyrE at 4203.973 kb on + strand, within pyrE at 4203.974 kb on - strand, within pyrE at 4203.979 kb on - strand, within pyrE at 4203.979 kb on - strand, within pyrE at 4204.035 kb on - strand, within pyrE at 4204.042 kb on + strand, within pyrE at 4204.043 kb on - strand, within pyrE at 4204.043 kb on - strand, within pyrE at 4204.056 kb on + strand, within pyrE at 4204.057 kb on - strand, within pyrE at 4204.312 kb on + strand at 4204.312 kb on + strand at 4204.578 kb on + strand, within rph at 4204.579 kb on - strand, within rph at 4204.579 kb on - strand, within rph at 4204.583 kb on + strand, within rph at 4204.584 kb on - strand, within rph at 4204.584 kb on - strand, within rph
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS) remove 4,202,868 + +1.1 4,202,904 + -1.2 4,202,963 + -0.4 4,202,967 - -0.5 4,203,183 - slmA LU632_RS22010 0.33 -2.5 4,203,221 - slmA LU632_RS22010 0.39 -1.1 4,203,309 - slmA LU632_RS22010 0.54 -0.1 4,203,371 - slmA LU632_RS22010 0.64 -3.0 4,203,896 + pyrE LU632_RS22015 0.44 -1.1 4,203,897 - pyrE LU632_RS22015 0.44 +0.4 4,203,897 - pyrE LU632_RS22015 0.44 -0.4 4,203,899 + pyrE LU632_RS22015 0.44 +1.2 4,203,899 + pyrE LU632_RS22015 0.44 -0.9 4,203,973 + pyrE LU632_RS22015 0.56 -0.4 4,203,974 - pyrE LU632_RS22015 0.56 +0.2 4,203,979 - pyrE LU632_RS22015 0.57 -1.4 4,203,979 - pyrE LU632_RS22015 0.57 +0.2 4,204,035 - pyrE LU632_RS22015 0.65 -1.1 4,204,042 + pyrE LU632_RS22015 0.67 +1.2 4,204,043 - pyrE LU632_RS22015 0.67 -1.1 4,204,043 - pyrE LU632_RS22015 0.67 -3.0 4,204,056 + pyrE LU632_RS22015 0.69 -0.2 4,204,057 - pyrE LU632_RS22015 0.69 -0.8 4,204,312 + -0.8 4,204,312 + +1.2 4,204,578 + rph LU632_RS22020 0.37 -1.5 4,204,579 - rph LU632_RS22020 0.37 -0.7 4,204,579 - rph LU632_RS22020 0.37 +1.4 4,204,583 + rph LU632_RS22020 0.38 -2.0 4,204,584 - rph LU632_RS22020 0.38 +1.5 4,204,584 - rph LU632_RS22020 0.38 -0.4
Or see this region's nucleotide sequence