Experiment: Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheA and grcA are separated by 178 nucleotides grcA and ung are separated by 284 nucleotides
LU632_RS16960: pheA - bifunctional chorismate mutase/prephenate dehydratase, at 3,198,415 to 3,199,575
pheA
LU632_RS16965: grcA - autonomous glycyl radical cofactor GrcA, at 3,199,754 to 3,200,137
grcA
LU632_RS16970: ung - uracil-DNA glycosylase, at 3,200,422 to 3,201,099
ung
Position (kb)
3199
3200
3201 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3198.782 kb on - strand, within pheA at 3198.804 kb on + strand, within pheA at 3198.871 kb on + strand, within pheA at 3198.932 kb on + strand, within pheA at 3198.952 kb on - strand, within pheA at 3198.952 kb on - strand, within pheA at 3198.989 kb on + strand, within pheA at 3199.013 kb on + strand, within pheA at 3199.014 kb on - strand, within pheA at 3199.102 kb on + strand, within pheA at 3199.103 kb on - strand, within pheA at 3199.194 kb on - strand, within pheA at 3199.194 kb on - strand, within pheA at 3199.194 kb on - strand, within pheA at 3199.208 kb on + strand, within pheA at 3199.208 kb on + strand, within pheA at 3199.209 kb on - strand, within pheA at 3199.209 kb on - strand, within pheA at 3199.243 kb on + strand, within pheA at 3199.243 kb on + strand, within pheA at 3199.245 kb on + strand, within pheA at 3199.245 kb on + strand, within pheA at 3199.246 kb on - strand, within pheA at 3199.255 kb on - strand, within pheA at 3199.283 kb on + strand, within pheA at 3199.283 kb on + strand, within pheA at 3199.349 kb on + strand, within pheA at 3199.350 kb on - strand, within pheA at 3199.350 kb on - strand, within pheA at 3199.378 kb on - strand, within pheA at 3199.378 kb on - strand, within pheA at 3199.378 kb on - strand, within pheA at 3199.382 kb on + strand, within pheA at 3199.621 kb on - strand at 3199.670 kb on + strand at 3199.670 kb on + strand at 3199.693 kb on + strand at 3199.740 kb on + strand at 3200.045 kb on - strand, within grcA at 3200.045 kb on - strand, within grcA at 3200.137 kb on + strand at 3200.138 kb on - strand at 3200.154 kb on + strand at 3200.154 kb on + strand at 3200.158 kb on + strand at 3200.158 kb on + strand at 3200.158 kb on + strand at 3200.217 kb on - strand at 3200.218 kb on + strand at 3200.219 kb on - strand at 3200.219 kb on - strand at 3200.220 kb on + strand at 3200.220 kb on + strand at 3200.221 kb on - strand at 3200.402 kb on + strand at 3200.403 kb on - strand at 3200.499 kb on - strand, within ung at 3200.553 kb on - strand, within ung at 3200.579 kb on + strand, within ung at 3200.579 kb on + strand, within ung at 3200.583 kb on + strand, within ung at 3200.584 kb on - strand, within ung at 3200.588 kb on + strand, within ung at 3200.589 kb on - strand, within ung at 3200.695 kb on - strand, within ung at 3200.699 kb on - strand, within ung at 3200.700 kb on + strand, within ung at 3200.777 kb on + strand, within ung at 3200.780 kb on + strand, within ung at 3200.780 kb on + strand, within ung at 3200.780 kb on + strand, within ung at 3200.783 kb on + strand, within ung at 3200.788 kb on - strand, within ung at 3200.854 kb on - strand, within ung at 3200.868 kb on - strand, within ung at 3200.876 kb on + strand, within ung at 3200.901 kb on - strand, within ung at 3200.944 kb on - strand, within ung at 3200.962 kb on + strand, within ung at 3201.040 kb on - strand at 3201.098 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS) remove 3,198,782 - pheA LU632_RS16960 0.32 +0.1 3,198,804 + pheA LU632_RS16960 0.34 +1.1 3,198,871 + pheA LU632_RS16960 0.39 -0.3 3,198,932 + pheA LU632_RS16960 0.45 -0.5 3,198,952 - pheA LU632_RS16960 0.46 +1.1 3,198,952 - pheA LU632_RS16960 0.46 -0.9 3,198,989 + pheA LU632_RS16960 0.49 +0.1 3,199,013 + pheA LU632_RS16960 0.52 +1.1 3,199,014 - pheA LU632_RS16960 0.52 +1.1 3,199,102 + pheA LU632_RS16960 0.59 +1.1 3,199,103 - pheA LU632_RS16960 0.59 +0.1 3,199,194 - pheA LU632_RS16960 0.67 +1.7 3,199,194 - pheA LU632_RS16960 0.67 +0.1 3,199,194 - pheA LU632_RS16960 0.67 -0.8 3,199,208 + pheA LU632_RS16960 0.68 +2.3 3,199,208 + pheA LU632_RS16960 0.68 +1.5 3,199,209 - pheA LU632_RS16960 0.68 -1.5 3,199,209 - pheA LU632_RS16960 0.68 -0.7 3,199,243 + pheA LU632_RS16960 0.71 +0.6 3,199,243 + pheA LU632_RS16960 0.71 -0.5 3,199,245 + pheA LU632_RS16960 0.71 -1.9 3,199,245 + pheA LU632_RS16960 0.71 -1.3 3,199,246 - pheA LU632_RS16960 0.72 +0.4 3,199,255 - pheA LU632_RS16960 0.72 +1.1 3,199,283 + pheA LU632_RS16960 0.75 +0.4 3,199,283 + pheA LU632_RS16960 0.75 -2.5 3,199,349 + pheA LU632_RS16960 0.80 -0.9 3,199,350 - pheA LU632_RS16960 0.81 +0.3 3,199,350 - pheA LU632_RS16960 0.81 +1.5 3,199,378 - pheA LU632_RS16960 0.83 -1.9 3,199,378 - pheA LU632_RS16960 0.83 +1.1 3,199,378 - pheA LU632_RS16960 0.83 +1.5 3,199,382 + pheA LU632_RS16960 0.83 +0.3 3,199,621 - -0.8 3,199,670 + +1.1 3,199,670 + +1.6 3,199,693 + +1.1 3,199,740 + +1.3 3,200,045 - grcA LU632_RS16965 0.76 -1.3 3,200,045 - grcA LU632_RS16965 0.76 -1.4 3,200,137 + -1.5 3,200,138 - +1.7 3,200,154 + -0.2 3,200,154 + +0.2 3,200,158 + +2.5 3,200,158 + -1.4 3,200,158 + -1.3 3,200,217 - -0.1 3,200,218 + -0.2 3,200,219 - +0.2 3,200,219 - +0.2 3,200,220 + +0.2 3,200,220 + -2.3 3,200,221 - -1.3 3,200,402 + +0.1 3,200,403 - +0.3 3,200,499 - ung LU632_RS16970 0.11 -0.9 3,200,553 - ung LU632_RS16970 0.19 +2.7 3,200,579 + ung LU632_RS16970 0.23 -2.4 3,200,579 + ung LU632_RS16970 0.23 +0.7 3,200,583 + ung LU632_RS16970 0.24 -1.1 3,200,584 - ung LU632_RS16970 0.24 -0.9 3,200,588 + ung LU632_RS16970 0.24 -1.3 3,200,589 - ung LU632_RS16970 0.25 -0.9 3,200,695 - ung LU632_RS16970 0.40 +0.7 3,200,699 - ung LU632_RS16970 0.41 -0.3 3,200,700 + ung LU632_RS16970 0.41 -0.4 3,200,777 + ung LU632_RS16970 0.52 -0.3 3,200,780 + ung LU632_RS16970 0.53 +1.8 3,200,780 + ung LU632_RS16970 0.53 +2.4 3,200,780 + ung LU632_RS16970 0.53 -1.7 3,200,783 + ung LU632_RS16970 0.53 -1.3 3,200,788 - ung LU632_RS16970 0.54 +0.3 3,200,854 - ung LU632_RS16970 0.64 -0.5 3,200,868 - ung LU632_RS16970 0.66 +1.0 3,200,876 + ung LU632_RS16970 0.67 +1.2 3,200,901 - ung LU632_RS16970 0.71 -2.4 3,200,944 - ung LU632_RS16970 0.77 +1.3 3,200,962 + ung LU632_RS16970 0.80 -1.1 3,201,040 - +0.4 3,201,098 - +2.2
Or see this region's nucleotide sequence