Experiment: Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purM and upp are separated by 242 nucleotides upp and LU632_RS08285 overlap by 1 nucleotides LU632_RS08285 and uraA are separated by 60 nucleotides
LU632_RS08275: purM - phosphoribosylformylglycinamidine cyclo-ligase, at 1,556,194 to 1,557,234
purM
LU632_RS08280: upp - uracil phosphoribosyltransferase, at 1,557,477 to 1,558,103
upp
LU632_RS08285: LU632_RS08285 - hypothetical protein, at 1,558,103 to 1,558,258
_RS08285
LU632_RS08290: uraA - uracil permease, at 1,558,319 to 1,559,596
uraA
Position (kb) 1557
1558
1559 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1556.502 kb on + strand, within purM at 1556.550 kb on - strand, within purM at 1556.553 kb on + strand, within purM at 1556.589 kb on + strand, within purM at 1556.774 kb on + strand, within purM at 1556.774 kb on + strand, within purM at 1556.789 kb on + strand, within purM at 1556.790 kb on - strand, within purM at 1556.909 kb on + strand, within purM at 1556.909 kb on + strand, within purM at 1556.909 kb on + strand, within purM at 1556.909 kb on + strand, within purM at 1556.910 kb on - strand, within purM at 1556.910 kb on - strand, within purM at 1556.912 kb on + strand, within purM at 1556.912 kb on + strand, within purM at 1556.913 kb on - strand, within purM at 1557.157 kb on - strand at 1557.209 kb on + strand at 1557.209 kb on + strand at 1557.280 kb on - strand at 1557.295 kb on + strand at 1557.402 kb on - strand at 1557.406 kb on - strand at 1557.406 kb on - strand at 1557.406 kb on - strand at 1557.433 kb on + strand at 1557.434 kb on + strand at 1557.495 kb on - strand at 1557.515 kb on + strand at 1557.516 kb on - strand at 1557.761 kb on + strand, within upp at 1557.761 kb on + strand, within upp at 1557.762 kb on - strand, within upp at 1557.899 kb on + strand, within upp at 1558.012 kb on - strand, within upp at 1558.101 kb on + strand at 1558.107 kb on + strand at 1558.107 kb on + strand at 1558.108 kb on - strand at 1558.191 kb on - strand, within LU632_RS08285 at 1558.191 kb on - strand, within LU632_RS08285 at 1558.257 kb on - strand at 1558.257 kb on - strand at 1558.268 kb on - strand at 1558.303 kb on + strand at 1558.316 kb on - strand at 1558.392 kb on + strand at 1558.496 kb on - strand, within uraA at 1558.578 kb on - strand, within uraA at 1558.864 kb on + strand, within uraA at 1558.865 kb on - strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.974 kb on - strand, within uraA at 1559.073 kb on + strand, within uraA at 1559.074 kb on - strand, within uraA
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS) remove 1,556,502 + purM LU632_RS08275 0.30 +0.9 1,556,550 - purM LU632_RS08275 0.34 -1.3 1,556,553 + purM LU632_RS08275 0.34 -0.1 1,556,589 + purM LU632_RS08275 0.38 +1.2 1,556,774 + purM LU632_RS08275 0.56 -2.5 1,556,774 + purM LU632_RS08275 0.56 -0.2 1,556,789 + purM LU632_RS08275 0.57 -0.7 1,556,790 - purM LU632_RS08275 0.57 -2.2 1,556,909 + purM LU632_RS08275 0.69 -1.1 1,556,909 + purM LU632_RS08275 0.69 -2.5 1,556,909 + purM LU632_RS08275 0.69 -2.6 1,556,909 + purM LU632_RS08275 0.69 +1.2 1,556,910 - purM LU632_RS08275 0.69 +0.1 1,556,910 - purM LU632_RS08275 0.69 +0.7 1,556,912 + purM LU632_RS08275 0.69 +1.2 1,556,912 + purM LU632_RS08275 0.69 -0.3 1,556,913 - purM LU632_RS08275 0.69 +1.2 1,557,157 - -1.2 1,557,209 + +1.2 1,557,209 + -0.4 1,557,280 - +1.8 1,557,295 + +0.2 1,557,402 - -0.6 1,557,406 - -1.8 1,557,406 - -0.8 1,557,406 - +0.7 1,557,433 + -1.2 1,557,434 + +1.1 1,557,495 - +2.0 1,557,515 + +0.2 1,557,516 - +1.8 1,557,761 + upp LU632_RS08280 0.45 -1.2 1,557,761 + upp LU632_RS08280 0.45 +0.2 1,557,762 - upp LU632_RS08280 0.45 -1.5 1,557,899 + upp LU632_RS08280 0.67 -1.0 1,558,012 - upp LU632_RS08280 0.85 +0.0 1,558,101 + +0.0 1,558,107 + +1.4 1,558,107 + +1.6 1,558,108 - +1.7 1,558,191 - LU632_RS08285 0.56 -1.1 1,558,191 - LU632_RS08285 0.56 -1.4 1,558,257 - +1.2 1,558,257 - -0.8 1,558,268 - -0.4 1,558,303 + +0.3 1,558,316 - -0.4 1,558,392 + -0.7 1,558,496 - uraA LU632_RS08290 0.14 -3.3 1,558,578 - uraA LU632_RS08290 0.20 +0.9 1,558,864 + uraA LU632_RS08290 0.43 -0.4 1,558,865 - uraA LU632_RS08290 0.43 +1.2 1,558,897 + uraA LU632_RS08290 0.45 +1.2 1,558,897 + uraA LU632_RS08290 0.45 -2.3 1,558,897 + uraA LU632_RS08290 0.45 -2.4 1,558,897 + uraA LU632_RS08290 0.45 -1.5 1,558,897 + uraA LU632_RS08290 0.45 -1.0 1,558,897 + uraA LU632_RS08290 0.45 -0.6 1,558,897 + uraA LU632_RS08290 0.45 -0.4 1,558,897 + uraA LU632_RS08290 0.45 -0.0 1,558,897 + uraA LU632_RS08290 0.45 -0.4 1,558,897 + uraA LU632_RS08290 0.45 -0.1 1,558,897 + uraA LU632_RS08290 0.45 +2.6 1,558,897 + uraA LU632_RS08290 0.45 -0.9 1,558,898 - uraA LU632_RS08290 0.45 -4.2 1,558,898 - uraA LU632_RS08290 0.45 -0.7 1,558,898 - uraA LU632_RS08290 0.45 -0.2 1,558,898 - uraA LU632_RS08290 0.45 +0.8 1,558,898 - uraA LU632_RS08290 0.45 +0.8 1,558,898 - uraA LU632_RS08290 0.45 +0.2 1,558,898 - uraA LU632_RS08290 0.45 +0.6 1,558,974 - uraA LU632_RS08290 0.51 +2.2 1,559,073 + uraA LU632_RS08290 0.59 -0.4 1,559,074 - uraA LU632_RS08290 0.59 -0.7
Or see this region's nucleotide sequence