Experiment: Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygfZ and sdhE are separated by 216 nucleotides sdhE and LU632_RS05965 overlap by 20 nucleotides LU632_RS05965 and fldB are separated by 109 nucleotides fldB and xerD are separated by 98 nucleotides
LU632_RS05955: ygfZ - tRNA-modifying protein YgfZ, at 1,129,015 to 1,129,995
ygfZ
LU632_RS05960: sdhE - FAD assembly factor SdhE, at 1,130,212 to 1,130,478
sdhE
LU632_RS05965: LU632_RS05965 - hypothetical protein, at 1,130,459 to 1,130,860
_RS05965
LU632_RS05970: fldB - flavodoxin FldB, at 1,130,970 to 1,131,488
fldB
LU632_RS05975: xerD - site-specific tyrosine recombinase XerD, at 1,131,587 to 1,132,480
xerD
Position (kb)
1130
1131
1132 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1130.091 kb on - strand at 1130.218 kb on - strand at 1130.218 kb on - strand at 1130.219 kb on + strand at 1130.220 kb on - strand at 1130.220 kb on - strand at 1130.223 kb on + strand at 1130.224 kb on - strand at 1130.274 kb on + strand, within sdhE at 1130.280 kb on + strand, within sdhE at 1130.299 kb on + strand, within sdhE at 1130.342 kb on + strand, within sdhE at 1130.376 kb on + strand, within sdhE at 1130.424 kb on + strand, within sdhE at 1130.570 kb on + strand, within LU632_RS05965 at 1130.570 kb on + strand, within LU632_RS05965 at 1130.571 kb on - strand, within LU632_RS05965 at 1130.571 kb on - strand, within LU632_RS05965 at 1130.573 kb on - strand, within LU632_RS05965 at 1130.607 kb on + strand, within LU632_RS05965 at 1130.608 kb on - strand, within LU632_RS05965 at 1130.745 kb on + strand, within LU632_RS05965 at 1130.803 kb on + strand, within LU632_RS05965 at 1130.803 kb on + strand, within LU632_RS05965 at 1130.803 kb on + strand, within LU632_RS05965 at 1130.803 kb on + strand, within LU632_RS05965 at 1130.803 kb on + strand, within LU632_RS05965 at 1130.856 kb on + strand at 1130.856 kb on + strand at 1130.857 kb on - strand at 1130.858 kb on + strand at 1130.863 kb on + strand at 1130.863 kb on + strand at 1130.864 kb on - strand at 1130.897 kb on + strand at 1130.945 kb on + strand at 1130.945 kb on + strand at 1130.952 kb on + strand at 1130.977 kb on - strand at 1131.141 kb on + strand, within fldB at 1131.173 kb on + strand, within fldB at 1131.173 kb on + strand, within fldB at 1131.173 kb on + strand, within fldB at 1131.173 kb on + strand, within fldB at 1131.174 kb on - strand, within fldB at 1131.174 kb on - strand, within fldB at 1131.175 kb on + strand, within fldB at 1131.175 kb on + strand, within fldB at 1131.175 kb on + strand, within fldB at 1131.175 kb on + strand, within fldB at 1131.175 kb on + strand, within fldB at 1131.176 kb on - strand, within fldB at 1131.176 kb on - strand, within fldB at 1131.327 kb on - strand, within fldB at 1131.352 kb on + strand, within fldB at 1131.390 kb on - strand, within fldB at 1131.452 kb on - strand at 1131.452 kb on - strand at 1131.452 kb on - strand at 1131.452 kb on - strand at 1131.452 kb on - strand at 1131.528 kb on + strand at 1132.010 kb on + strand, within xerD at 1132.010 kb on + strand, within xerD at 1132.010 kb on + strand, within xerD at 1132.159 kb on + strand, within xerD at 1132.425 kb on - strand at 1132.452 kb on + strand at 1132.452 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS) remove 1,130,091 - +0.9 1,130,218 - -1.3 1,130,218 - +1.1 1,130,219 + -0.9 1,130,220 - +0.4 1,130,220 - -1.1 1,130,223 + -1.3 1,130,224 - -2.4 1,130,274 + sdhE LU632_RS05960 0.23 -2.6 1,130,280 + sdhE LU632_RS05960 0.25 +0.6 1,130,299 + sdhE LU632_RS05960 0.33 +1.1 1,130,342 + sdhE LU632_RS05960 0.49 -0.1 1,130,376 + sdhE LU632_RS05960 0.61 -1.3 1,130,424 + sdhE LU632_RS05960 0.79 -1.5 1,130,570 + LU632_RS05965 0.28 -1.1 1,130,570 + LU632_RS05965 0.28 -0.5 1,130,571 - LU632_RS05965 0.28 +1.1 1,130,571 - LU632_RS05965 0.28 -0.4 1,130,573 - LU632_RS05965 0.28 -1.1 1,130,607 + LU632_RS05965 0.37 +4.3 1,130,608 - LU632_RS05965 0.37 +1.1 1,130,745 + LU632_RS05965 0.71 -0.0 1,130,803 + LU632_RS05965 0.86 -0.5 1,130,803 + LU632_RS05965 0.86 +0.1 1,130,803 + LU632_RS05965 0.86 -1.9 1,130,803 + LU632_RS05965 0.86 -0.5 1,130,803 + LU632_RS05965 0.86 -0.5 1,130,856 + +0.1 1,130,856 + +0.0 1,130,857 - +0.3 1,130,858 + -0.4 1,130,863 + +1.1 1,130,863 + -1.5 1,130,864 - -2.2 1,130,897 + +0.2 1,130,945 + -1.7 1,130,945 + -0.3 1,130,952 + +0.1 1,130,977 - +1.1 1,131,141 + fldB LU632_RS05970 0.33 -1.3 1,131,173 + fldB LU632_RS05970 0.39 -1.3 1,131,173 + fldB LU632_RS05970 0.39 -2.5 1,131,173 + fldB LU632_RS05970 0.39 +0.1 1,131,173 + fldB LU632_RS05970 0.39 -0.1 1,131,174 - fldB LU632_RS05970 0.39 +1.6 1,131,174 - fldB LU632_RS05970 0.39 -0.2 1,131,175 + fldB LU632_RS05970 0.39 -0.9 1,131,175 + fldB LU632_RS05970 0.39 +1.1 1,131,175 + fldB LU632_RS05970 0.39 -1.5 1,131,175 + fldB LU632_RS05970 0.39 -0.1 1,131,175 + fldB LU632_RS05970 0.39 +0.6 1,131,176 - fldB LU632_RS05970 0.40 +1.1 1,131,176 - fldB LU632_RS05970 0.40 +1.6 1,131,327 - fldB LU632_RS05970 0.69 -0.1 1,131,352 + fldB LU632_RS05970 0.74 -0.6 1,131,390 - fldB LU632_RS05970 0.81 -0.3 1,131,452 - -1.3 1,131,452 - +0.2 1,131,452 - +0.2 1,131,452 - +1.1 1,131,452 - -0.1 1,131,528 + -2.1 1,132,010 + xerD LU632_RS05975 0.47 +1.1 1,132,010 + xerD LU632_RS05975 0.47 -2.5 1,132,010 + xerD LU632_RS05975 0.47 -1.3 1,132,159 + xerD LU632_RS05975 0.64 -0.9 1,132,425 - +0.6 1,132,452 + +4.4 1,132,452 + +1.1
Or see this region's nucleotide sequence