Experiment: Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nnr and tsaE overlap by 1 nucleotides tsaE and amiB are separated by 11 nucleotides amiB and mutL are separated by 70 nucleotides
LU632_RS02030: nnr - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 397,401 to 398,921
nnr
LU632_RS02035: tsaE - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 398,921 to 399,394
tsaE
LU632_RS02040: amiB - N-acetylmuramoyl-L-alanine amidase AmiB, at 399,406 to 401,067
amiB
LU632_RS02045: mutL - DNA mismatch repair endonuclease MutL, at 401,138 to 403,030
mutL
Position (kb) 399
400
401
402 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 398.420 kb on - strand, within nnr at 398.447 kb on + strand, within nnr at 398.447 kb on + strand, within nnr at 398.448 kb on - strand, within nnr at 398.556 kb on - strand, within nnr at 398.556 kb on - strand, within nnr at 398.630 kb on - strand, within nnr at 398.631 kb on + strand, within nnr at 400.719 kb on + strand, within amiB at 400.792 kb on + strand, within amiB at 400.792 kb on + strand, within amiB at 400.899 kb on + strand, within amiB at 400.932 kb on + strand at 400.933 kb on - strand at 400.937 kb on + strand at 400.937 kb on + strand at 400.937 kb on + strand at 400.937 kb on + strand at 400.938 kb on - strand at 400.938 kb on - strand at 400.938 kb on - strand at 400.940 kb on + strand at 400.941 kb on - strand at 400.941 kb on - strand at 400.941 kb on - strand at 401.013 kb on + strand at 401.013 kb on + strand at 401.014 kb on - strand at 401.049 kb on - strand at 401.049 kb on - strand at 401.052 kb on + strand at 401.134 kb on + strand at 401.137 kb on + strand at 401.155 kb on - strand at 401.324 kb on - strand at 401.405 kb on - strand, within mutL at 401.507 kb on + strand, within mutL at 401.507 kb on + strand, within mutL at 401.508 kb on - strand, within mutL at 401.508 kb on - strand, within mutL at 401.571 kb on + strand, within mutL at 401.648 kb on - strand, within mutL at 401.717 kb on - strand, within mutL at 401.897 kb on + strand, within mutL at 401.903 kb on + strand, within mutL at 401.917 kb on + strand, within mutL at 401.918 kb on - strand, within mutL at 401.918 kb on - strand, within mutL at 401.918 kb on - strand, within mutL at 401.918 kb on - strand, within mutL at 401.996 kb on + strand, within mutL at 401.996 kb on + strand, within mutL at 401.996 kb on + strand, within mutL at 401.996 kb on - strand, within mutL at 401.997 kb on - strand, within mutL at 401.997 kb on - strand, within mutL at 401.997 kb on - strand, within mutL at 402.000 kb on + strand, within mutL at 402.000 kb on + strand, within mutL at 402.000 kb on + strand, within mutL at 402.000 kb on + strand, within mutL at 402.001 kb on - strand, within mutL at 402.001 kb on - strand, within mutL at 402.001 kb on - strand, within mutL at 402.005 kb on + strand, within mutL at 402.042 kb on + strand, within mutL at 402.042 kb on + strand, within mutL
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS) remove 398,420 - nnr LU632_RS02030 0.67 +2.3 398,447 + nnr LU632_RS02030 0.69 +0.2 398,447 + nnr LU632_RS02030 0.69 +0.5 398,448 - nnr LU632_RS02030 0.69 +1.9 398,556 - nnr LU632_RS02030 0.76 +1.9 398,556 - nnr LU632_RS02030 0.76 +0.0 398,630 - nnr LU632_RS02030 0.81 -1.7 398,631 + nnr LU632_RS02030 0.81 +2.5 400,719 + amiB LU632_RS02040 0.79 -2.5 400,792 + amiB LU632_RS02040 0.83 -2.2 400,792 + amiB LU632_RS02040 0.83 -2.3 400,899 + amiB LU632_RS02040 0.90 -3.2 400,932 + -1.5 400,933 - -0.8 400,937 + +1.7 400,937 + +0.1 400,937 + -2.2 400,937 + +0.5 400,938 - -0.6 400,938 - +1.6 400,938 - +0.6 400,940 + -1.9 400,941 - -1.0 400,941 - +0.5 400,941 - +1.3 401,013 + +1.3 401,013 + -0.2 401,014 - -0.7 401,049 - -0.8 401,049 - +0.8 401,052 + +3.9 401,134 + +1.5 401,137 + +0.6 401,155 - +1.5 401,324 - -0.7 401,405 - mutL LU632_RS02045 0.14 -0.3 401,507 + mutL LU632_RS02045 0.19 -0.7 401,507 + mutL LU632_RS02045 0.19 -1.7 401,508 - mutL LU632_RS02045 0.20 +0.3 401,508 - mutL LU632_RS02045 0.20 -3.9 401,571 + mutL LU632_RS02045 0.23 -0.8 401,648 - mutL LU632_RS02045 0.27 -2.2 401,717 - mutL LU632_RS02045 0.31 +0.7 401,897 + mutL LU632_RS02045 0.40 +1.3 401,903 + mutL LU632_RS02045 0.40 -0.4 401,917 + mutL LU632_RS02045 0.41 +1.5 401,918 - mutL LU632_RS02045 0.41 +0.9 401,918 - mutL LU632_RS02045 0.41 +2.9 401,918 - mutL LU632_RS02045 0.41 -0.1 401,918 - mutL LU632_RS02045 0.41 -0.3 401,996 + mutL LU632_RS02045 0.45 +0.3 401,996 + mutL LU632_RS02045 0.45 -0.3 401,996 + mutL LU632_RS02045 0.45 +2.1 401,996 - mutL LU632_RS02045 0.45 +0.3 401,997 - mutL LU632_RS02045 0.45 +1.3 401,997 - mutL LU632_RS02045 0.45 -0.8 401,997 - mutL LU632_RS02045 0.45 +0.2 402,000 + mutL LU632_RS02045 0.46 -0.4 402,000 + mutL LU632_RS02045 0.46 -0.9 402,000 + mutL LU632_RS02045 0.46 +2.9 402,000 + mutL LU632_RS02045 0.46 -0.5 402,001 - mutL LU632_RS02045 0.46 -0.3 402,001 - mutL LU632_RS02045 0.46 -1.4 402,001 - mutL LU632_RS02045 0.46 -0.5 402,005 + mutL LU632_RS02045 0.46 -1.5 402,042 + mutL LU632_RS02045 0.48 -0.9 402,042 + mutL LU632_RS02045 0.48 -0.9
Or see this region's nucleotide sequence