Experiment: Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemE and nfi are separated by 1 nucleotides nfi and LU632_RS00995 are separated by 67 nucleotides LU632_RS00995 and hupA are separated by 188 nucleotides hupA and tnpA are separated by 167 nucleotides
LU632_RS00985: hemE - uroporphyrinogen decarboxylase, at 213,528 to 214,595
hemE
LU632_RS00990: nfi - deoxyribonuclease V, at 214,597 to 215,265
nfi
LU632_RS00995: LU632_RS00995 - YjaG family protein, at 215,333 to 215,923
_RS00995
LU632_RS01000: hupA - DNA-binding protein HU-alpha, at 216,112 to 216,384
hupA
LU632_RS01005: tnpA - IS200/IS605 family transposase, at 216,552 to 216,992
tnpA
Position (kb) 215
216 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 214.614 kb on - strand at 214.641 kb on - strand at 214.788 kb on - strand, within nfi at 214.788 kb on - strand, within nfi at 214.813 kb on + strand, within nfi at 214.813 kb on + strand, within nfi at 214.813 kb on + strand, within nfi at 214.813 kb on + strand, within nfi at 214.813 kb on + strand, within nfi at 214.813 kb on + strand, within nfi at 214.814 kb on - strand, within nfi at 214.814 kb on - strand, within nfi at 214.814 kb on - strand, within nfi at 214.814 kb on - strand, within nfi at 214.815 kb on + strand, within nfi at 214.816 kb on - strand, within nfi at 214.816 kb on - strand, within nfi at 215.103 kb on + strand, within nfi at 215.104 kb on - strand, within nfi at 215.337 kb on + strand at 215.338 kb on - strand at 215.338 kb on - strand at 215.341 kb on + strand at 215.342 kb on - strand at 215.389 kb on + strand at 215.390 kb on - strand at 215.390 kb on - strand at 215.430 kb on - strand, within LU632_RS00995 at 215.483 kb on - strand, within LU632_RS00995 at 215.530 kb on + strand, within LU632_RS00995 at 215.585 kb on + strand, within LU632_RS00995 at 215.595 kb on - strand, within LU632_RS00995 at 215.603 kb on + strand, within LU632_RS00995 at 215.656 kb on - strand, within LU632_RS00995 at 215.856 kb on + strand, within LU632_RS00995 at 215.856 kb on + strand, within LU632_RS00995 at 215.856 kb on + strand, within LU632_RS00995 at 215.857 kb on - strand, within LU632_RS00995 at 215.955 kb on + strand at 215.994 kb on - strand at 216.032 kb on + strand at 216.032 kb on + strand at 216.033 kb on - strand at 216.093 kb on + strand at 216.093 kb on + strand at 216.094 kb on - strand at 216.094 kb on - strand at 216.094 kb on - strand at 216.112 kb on - strand at 216.137 kb on + strand at 216.174 kb on + strand, within hupA at 216.261 kb on + strand, within hupA at 216.262 kb on - strand, within hupA at 216.378 kb on + strand at 216.382 kb on + strand at 216.382 kb on + strand at 216.406 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS) remove 214,614 - -1.1 214,641 - -0.3 214,788 - nfi LU632_RS00990 0.29 -1.1 214,788 - nfi LU632_RS00990 0.29 -2.0 214,813 + nfi LU632_RS00990 0.32 -2.0 214,813 + nfi LU632_RS00990 0.32 +0.8 214,813 + nfi LU632_RS00990 0.32 +2.2 214,813 + nfi LU632_RS00990 0.32 -0.4 214,813 + nfi LU632_RS00990 0.32 -1.5 214,813 + nfi LU632_RS00990 0.32 +0.5 214,814 - nfi LU632_RS00990 0.32 -0.7 214,814 - nfi LU632_RS00990 0.32 +1.2 214,814 - nfi LU632_RS00990 0.32 +2.8 214,814 - nfi LU632_RS00990 0.32 +1.4 214,815 + nfi LU632_RS00990 0.33 +1.2 214,816 - nfi LU632_RS00990 0.33 +3.7 214,816 - nfi LU632_RS00990 0.33 -1.4 215,103 + nfi LU632_RS00990 0.76 -0.2 215,104 - nfi LU632_RS00990 0.76 +0.3 215,337 + -0.8 215,338 - +0.2 215,338 - +0.1 215,341 + -1.1 215,342 - +0.6 215,389 + -0.8 215,390 - -0.5 215,390 - -1.5 215,430 - LU632_RS00995 0.16 -1.4 215,483 - LU632_RS00995 0.25 -1.4 215,530 + LU632_RS00995 0.33 -1.4 215,585 + LU632_RS00995 0.43 -0.3 215,595 - LU632_RS00995 0.44 -2.1 215,603 + LU632_RS00995 0.46 -1.2 215,656 - LU632_RS00995 0.55 -2.4 215,856 + LU632_RS00995 0.88 -0.5 215,856 + LU632_RS00995 0.88 -0.4 215,856 + LU632_RS00995 0.88 +0.2 215,857 - LU632_RS00995 0.89 -1.2 215,955 + +0.0 215,994 - -1.3 216,032 + +1.4 216,032 + +1.7 216,033 - -0.5 216,093 + +0.7 216,093 + -1.6 216,094 - +0.4 216,094 - -0.1 216,094 - -0.8 216,112 - +0.4 216,137 + +2.3 216,174 + hupA LU632_RS01000 0.23 +1.6 216,261 + hupA LU632_RS01000 0.55 -1.3 216,262 - hupA LU632_RS01000 0.55 -0.5 216,378 + +1.0 216,382 + -0.4 216,382 + -0.1 216,406 + -0.3
Or see this region's nucleotide sequence