Experiment: Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS00485 and LU632_RS00490 overlap by 11 nucleotides LU632_RS00490 and sctS overlap by 4 nucleotides sctS and sctT are separated by 12 nucleotides sctT and LU632_RS00505 are separated by 9 nucleotides
LU632_RS00485: LU632_RS00485 - FliM/FliN family flagellar motor switch protein, at 100,068 to 100,994
_RS00485
LU632_RS00490: LU632_RS00490 - EscR/YscR/HrcR family type III secretion system export apparatus protein, at 100,984 to 101,655
_RS00490
LU632_RS00495: sctS - type III secretion system export apparatus subunit SctS, at 101,652 to 101,906
sctS
LU632_RS00500: sctT - type III secretion system export apparatus subunit SctT, at 101,919 to 102,683
sctT
LU632_RS00505: LU632_RS00505 - EscU/YscU/HrcU family type III secretion system export apparatus switch protein, at 102,693 to 103,784
_RS00505
Position (kb)
101
102 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 100.751 kb on - strand, within LU632_RS00485 at 101.059 kb on - strand, within LU632_RS00490 at 101.096 kb on + strand, within LU632_RS00490 at 101.459 kb on + strand, within LU632_RS00490 at 101.750 kb on - strand, within sctS at 101.905 kb on - strand at 101.905 kb on - strand at 101.938 kb on - strand at 101.955 kb on - strand at 102.007 kb on + strand, within sctT at 102.010 kb on + strand, within sctT at 102.050 kb on - strand, within sctT at 102.110 kb on + strand, within sctT at 102.345 kb on - strand, within sctT at 102.348 kb on + strand, within sctT at 102.349 kb on - strand, within sctT at 102.349 kb on - strand, within sctT at 102.352 kb on + strand, within sctT at 102.465 kb on - strand, within sctT at 102.465 kb on - strand, within sctT at 102.467 kb on - strand, within sctT
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 9 Main Stem (TE2-S-C9-MS) remove 100,751 - LU632_RS00485 0.74 -3.1 101,059 - LU632_RS00490 0.11 -0.3 101,096 + LU632_RS00490 0.17 +1.5 101,459 + LU632_RS00490 0.71 +2.2 101,750 - sctS LU632_RS00495 0.38 -3.2 101,905 - -2.1 101,905 - +0.0 101,938 - +0.3 101,955 - +1.1 102,007 + sctT LU632_RS00500 0.12 +1.7 102,010 + sctT LU632_RS00500 0.12 +0.9 102,050 - sctT LU632_RS00500 0.17 +0.1 102,110 + sctT LU632_RS00500 0.25 -0.6 102,345 - sctT LU632_RS00500 0.56 -0.8 102,348 + sctT LU632_RS00500 0.56 +0.4 102,349 - sctT LU632_RS00500 0.56 +1.0 102,349 - sctT LU632_RS00500 0.56 +0.3 102,352 + sctT LU632_RS00500 0.57 -2.0 102,465 - sctT LU632_RS00500 0.71 +0.2 102,465 - sctT LU632_RS00500 0.71 -1.6 102,467 - sctT LU632_RS00500 0.72 -1.6
Or see this region's nucleotide sequence