Experiment: Experiment:TE2, Cucumber 9 Petiole 1 (TE2-S-C9-P1)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuB and leuA are separated by 2 nucleotides leuA and ilvI are separated by 902 nucleotides
LU632_RS02875: leuB - 3-isopropylmalate dehydrogenase, at 584,552 to 585,640
leuB
LU632_RS02880: leuA - 2-isopropylmalate synthase, at 585,643 to 587,211
leuA
LU632_RS02885: ilvI - acetolactate synthase 3 large subunit, at 588,114 to 589,838
ilvI
Position (kb) 585
586
587
588 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 584.707 kb on + strand, within leuB at 584.720 kb on + strand, within leuB at 584.721 kb on - strand, within leuB at 584.965 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.967 kb on + strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 584.968 kb on - strand, within leuB at 585.119 kb on - strand, within leuB at 585.207 kb on + strand, within leuB at 585.208 kb on - strand, within leuB at 585.208 kb on - strand, within leuB at 585.460 kb on + strand, within leuB at 585.622 kb on + strand at 585.622 kb on + strand at 585.623 kb on - strand at 585.623 kb on - strand at 585.623 kb on - strand at 585.627 kb on + strand at 585.627 kb on + strand at 585.627 kb on + strand at 585.628 kb on - strand at 585.649 kb on + strand at 585.650 kb on - strand at 585.761 kb on - strand at 585.761 kb on - strand at 585.763 kb on - strand at 585.803 kb on + strand, within leuA at 585.803 kb on + strand, within leuA at 585.804 kb on - strand, within leuA at 585.820 kb on + strand, within leuA at 585.867 kb on - strand, within leuA at 585.882 kb on + strand, within leuA at 586.001 kb on - strand, within leuA at 586.103 kb on - strand, within leuA at 586.142 kb on + strand, within leuA at 586.142 kb on + strand, within leuA at 586.160 kb on + strand, within leuA at 586.160 kb on + strand, within leuA at 586.173 kb on + strand, within leuA at 586.173 kb on + strand, within leuA at 586.173 kb on + strand, within leuA at 586.173 kb on + strand, within leuA at 586.175 kb on + strand, within leuA at 586.191 kb on + strand, within leuA at 586.301 kb on - strand, within leuA at 586.371 kb on + strand, within leuA at 586.462 kb on + strand, within leuA at 586.462 kb on + strand, within leuA at 586.463 kb on - strand, within leuA at 586.531 kb on - strand, within leuA at 586.592 kb on - strand, within leuA at 586.605 kb on + strand, within leuA at 586.673 kb on + strand, within leuA at 586.692 kb on - strand, within leuA at 586.710 kb on + strand, within leuA at 586.710 kb on + strand, within leuA at 586.711 kb on + strand, within leuA at 586.711 kb on - strand, within leuA at 586.711 kb on - strand, within leuA at 586.711 kb on - strand, within leuA at 586.817 kb on + strand, within leuA at 586.818 kb on - strand, within leuA at 586.921 kb on - strand, within leuA at 586.971 kb on + strand, within leuA at 586.972 kb on - strand, within leuA at 587.032 kb on - strand, within leuA at 587.032 kb on - strand, within leuA at 587.092 kb on + strand at 587.093 kb on - strand at 587.093 kb on - strand at 587.198 kb on - strand at 587.348 kb on + strand at 587.351 kb on + strand at 587.351 kb on + strand at 587.355 kb on - strand at 587.461 kb on + strand at 587.463 kb on + strand at 587.463 kb on + strand at 587.464 kb on - strand at 587.464 kb on - strand at 587.464 kb on - strand at 587.464 kb on - strand at 587.464 kb on - strand at 587.569 kb on - strand at 587.605 kb on + strand at 587.682 kb on - strand at 587.736 kb on + strand at 587.736 kb on + strand at 587.737 kb on - strand at 587.737 kb on - strand at 587.799 kb on + strand at 587.831 kb on + strand at 587.832 kb on - strand at 587.832 kb on - strand at 587.832 kb on - strand at 587.836 kb on - strand at 587.933 kb on - strand at 588.066 kb on - strand at 588.074 kb on + strand at 588.075 kb on - strand at 588.178 kb on + strand at 588.178 kb on + strand at 588.179 kb on - strand at 588.211 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 9 Petiole 1 (TE2-S-C9-P1) remove 584,707 + leuB LU632_RS02875 0.14 -3.2 584,720 + leuB LU632_RS02875 0.15 -1.5 584,721 - leuB LU632_RS02875 0.16 -3.1 584,965 + leuB LU632_RS02875 0.38 -1.7 584,967 + leuB LU632_RS02875 0.38 -1.0 584,967 + leuB LU632_RS02875 0.38 -0.2 584,967 + leuB LU632_RS02875 0.38 -0.2 584,967 + leuB LU632_RS02875 0.38 -1.3 584,967 + leuB LU632_RS02875 0.38 -2.5 584,967 + leuB LU632_RS02875 0.38 -4.1 584,967 + leuB LU632_RS02875 0.38 -0.2 584,967 + leuB LU632_RS02875 0.38 +0.8 584,967 + leuB LU632_RS02875 0.38 -0.9 584,967 + leuB LU632_RS02875 0.38 -2.9 584,967 + leuB LU632_RS02875 0.38 +0.1 584,967 + leuB LU632_RS02875 0.38 -0.5 584,967 + leuB LU632_RS02875 0.38 -0.4 584,967 + leuB LU632_RS02875 0.38 -1.5 584,968 - leuB LU632_RS02875 0.38 -0.8 584,968 - leuB LU632_RS02875 0.38 -1.2 584,968 - leuB LU632_RS02875 0.38 -2.7 584,968 - leuB LU632_RS02875 0.38 +1.3 584,968 - leuB LU632_RS02875 0.38 -2.4 584,968 - leuB LU632_RS02875 0.38 -1.5 584,968 - leuB LU632_RS02875 0.38 -0.4 584,968 - leuB LU632_RS02875 0.38 +0.8 584,968 - leuB LU632_RS02875 0.38 -1.7 584,968 - leuB LU632_RS02875 0.38 -0.3 584,968 - leuB LU632_RS02875 0.38 -2.2 584,968 - leuB LU632_RS02875 0.38 +0.8 584,968 - leuB LU632_RS02875 0.38 -1.4 584,968 - leuB LU632_RS02875 0.38 -0.8 585,119 - leuB LU632_RS02875 0.52 -1.8 585,207 + leuB LU632_RS02875 0.60 -1.7 585,208 - leuB LU632_RS02875 0.60 -1.2 585,208 - leuB LU632_RS02875 0.60 +1.1 585,460 + leuB LU632_RS02875 0.83 -1.5 585,622 + -1.0 585,622 + -1.2 585,623 - +0.1 585,623 - -1.4 585,623 - -0.5 585,627 + -1.0 585,627 + -0.8 585,627 + -3.0 585,628 - -0.1 585,649 + -1.2 585,650 - -2.9 585,761 - -1.0 585,761 - -2.4 585,763 - -1.6 585,803 + leuA LU632_RS02880 0.10 -1.8 585,803 + leuA LU632_RS02880 0.10 -1.1 585,804 - leuA LU632_RS02880 0.10 -1.2 585,820 + leuA LU632_RS02880 0.11 -0.8 585,867 - leuA LU632_RS02880 0.14 -2.2 585,882 + leuA LU632_RS02880 0.15 -1.7 586,001 - leuA LU632_RS02880 0.23 -0.8 586,103 - leuA LU632_RS02880 0.29 -1.0 586,142 + leuA LU632_RS02880 0.32 -0.1 586,142 + leuA LU632_RS02880 0.32 -1.2 586,160 + leuA LU632_RS02880 0.33 +1.0 586,160 + leuA LU632_RS02880 0.33 -0.4 586,173 + leuA LU632_RS02880 0.34 -0.2 586,173 + leuA LU632_RS02880 0.34 -0.4 586,173 + leuA LU632_RS02880 0.34 +0.2 586,173 + leuA LU632_RS02880 0.34 -1.0 586,175 + leuA LU632_RS02880 0.34 -1.3 586,191 + leuA LU632_RS02880 0.35 -0.2 586,301 - leuA LU632_RS02880 0.42 -2.1 586,371 + leuA LU632_RS02880 0.46 -0.7 586,462 + leuA LU632_RS02880 0.52 -2.5 586,462 + leuA LU632_RS02880 0.52 -0.2 586,463 - leuA LU632_RS02880 0.52 -1.3 586,531 - leuA LU632_RS02880 0.57 -1.2 586,592 - leuA LU632_RS02880 0.60 -1.6 586,605 + leuA LU632_RS02880 0.61 -0.2 586,673 + leuA LU632_RS02880 0.66 -0.4 586,692 - leuA LU632_RS02880 0.67 -1.2 586,710 + leuA LU632_RS02880 0.68 -0.5 586,710 + leuA LU632_RS02880 0.68 -2.2 586,711 + leuA LU632_RS02880 0.68 -1.5 586,711 - leuA LU632_RS02880 0.68 -2.7 586,711 - leuA LU632_RS02880 0.68 -1.3 586,711 - leuA LU632_RS02880 0.68 +0.1 586,817 + leuA LU632_RS02880 0.75 +0.8 586,818 - leuA LU632_RS02880 0.75 -1.5 586,921 - leuA LU632_RS02880 0.81 -0.8 586,971 + leuA LU632_RS02880 0.85 -1.1 586,972 - leuA LU632_RS02880 0.85 -0.0 587,032 - leuA LU632_RS02880 0.89 -1.5 587,032 - leuA LU632_RS02880 0.89 -0.8 587,092 + -0.6 587,093 - -2.8 587,093 - -2.7 587,198 - -3.5 587,348 + -1.6 587,351 + -3.4 587,351 + -2.0 587,355 - +0.8 587,461 + -0.7 587,463 + -0.5 587,463 + +5.1 587,464 - -0.6 587,464 - -0.8 587,464 - +0.8 587,464 - -0.4 587,464 - -0.5 587,569 - +0.5 587,605 + -0.8 587,682 - -0.6 587,736 + +0.4 587,736 + +1.5 587,737 - +2.1 587,737 - -1.6 587,799 + -1.1 587,831 + +1.9 587,832 - -1.2 587,832 - -1.9 587,832 - +2.3 587,836 - +0.5 587,933 - +0.5 588,066 - -1.0 588,074 + -0.6 588,075 - +0.6 588,178 + -1.7 588,178 + +0.5 588,179 - -0.3 588,211 + +0.3
Or see this region's nucleotide sequence