Experiment: NAG 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1181_RS03240 and HMPREF1181_RS03245 are separated by 138 nucleotides HMPREF1181_RS03245 and HMPREF1181_RS03250 are separated by 53 nucleotides HMPREF1181_RS03250 and HMPREF1181_RS03255 overlap by 4 nucleotides
HMPREF1181_RS03240: HMPREF1181_RS03240 - 50S ribosomal protein L34, at 828,672 to 828,833
_RS03240
HMPREF1181_RS03245: HMPREF1181_RS03245 - PASTA domain-containing protein, at 828,972 to 829,625
_RS03245
HMPREF1181_RS03250: HMPREF1181_RS03250 - RluA family pseudouridine synthase, at 829,679 to 830,749
_RS03250
HMPREF1181_RS03255: HMPREF1181_RS03255 - D-alanine--D-alanine ligase, at 830,746 to 831,720
_RS03255
Position (kb)
829
830
831 Strain fitness (log2 ratio)
-1
0
1 at 828.831 kb on + strand at 828.844 kb on + strand at 828.844 kb on + strand at 829.704 kb on + strand at 829.728 kb on + strand at 829.737 kb on + strand at 829.776 kb on + strand at 829.814 kb on + strand, within HMPREF1181_RS03250 at 829.990 kb on + strand, within HMPREF1181_RS03250 at 830.182 kb on + strand, within HMPREF1181_RS03250 at 830.182 kb on + strand, within HMPREF1181_RS03250 at 830.182 kb on + strand, within HMPREF1181_RS03250 at 830.481 kb on + strand, within HMPREF1181_RS03250 at 830.503 kb on + strand, within HMPREF1181_RS03250 at 830.725 kb on + strand at 830.728 kb on + strand at 831.718 kb on + strand at 831.729 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG 10 mM (C) remove 828,831 + -0.5 828,844 + -0.4 828,844 + +0.6 829,704 + -1.7 829,728 + -0.2 829,737 + +0.7 829,776 + -0.7 829,814 + HMPREF1181_RS03250 0.13 -1.2 829,990 + HMPREF1181_RS03250 0.29 -0.6 830,182 + HMPREF1181_RS03250 0.47 -0.9 830,182 + HMPREF1181_RS03250 0.47 -0.7 830,182 + HMPREF1181_RS03250 0.47 -0.6 830,481 + HMPREF1181_RS03250 0.75 -1.7 830,503 + HMPREF1181_RS03250 0.77 -0.2 830,725 + +0.1 830,728 + +0.6 831,718 + -0.1 831,729 + +0.1
Or see this region's nucleotide sequence