Experiment: Amylose from potato 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1181_RS03215 and HMPREF1181_RS03220 are separated by 22 nucleotides HMPREF1181_RS03220 and HMPREF1181_RS03225 are separated by 75 nucleotides HMPREF1181_RS03225 and HMPREF1181_RS03230 are separated by 37 nucleotides
HMPREF1181_RS03215: HMPREF1181_RS03215 - UDP-2,3-diacylglucosamine diphosphatase, at 824,496 to 825,260
_RS03215
HMPREF1181_RS03220: HMPREF1181_RS03220 - DUF59 domain-containing protein, at 825,283 to 825,594
_RS03220
HMPREF1181_RS03225: HMPREF1181_RS03225 - DNA repair protein RadC, at 825,670 to 826,356
_RS03225
HMPREF1181_RS03230: HMPREF1181_RS03230 - glycosyltransferase, at 826,394 to 827,383
_RS03230
Position (kb) 825
826
827 Strain fitness (log2 ratio) -1
0
1
2 at 825.491 kb on - strand, within HMPREF1181_RS03220 at 825.498 kb on - strand, within HMPREF1181_RS03220 at 825.648 kb on + strand at 825.701 kb on + strand at 825.721 kb on - strand at 825.721 kb on - strand at 825.926 kb on - strand, within HMPREF1181_RS03225 at 825.979 kb on + strand, within HMPREF1181_RS03225 at 826.017 kb on - strand, within HMPREF1181_RS03225 at 826.385 kb on + strand at 826.386 kb on - strand at 826.386 kb on - strand at 826.395 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Amylose from potato 2.5 mg/mL (C) remove 825,491 - HMPREF1181_RS03220 0.67 -0.6 825,498 - HMPREF1181_RS03220 0.69 +0.4 825,648 + +1.9 825,701 + +0.7 825,721 - +0.1 825,721 - +1.0 825,926 - HMPREF1181_RS03225 0.37 +0.5 825,979 + HMPREF1181_RS03225 0.45 +0.7 826,017 - HMPREF1181_RS03225 0.51 -1.1 826,385 + +0.2 826,386 - +0.3 826,386 - -0.3 826,395 + -1.0
Or see this region's nucleotide sequence