Experiment: Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wecC and wecB overlap by 4 nucleotides wecB and wzzE are separated by 49 nucleotides
LU632_RS24235: wecC - UDP-N-acetyl-D-mannosamine dehydrogenase, at 4,621,954 to 4,623,216
wecC
LU632_RS24240: wecB - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 4,623,213 to 4,624,343
wecB
LU632_RS24245: wzzE - ECA polysaccharide chain length modulation protein, at 4,624,393 to 4,625,421
wzzE
Position (kb)
4623
4624
4625 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4622.271 kb on + strand, within wecC at 4622.397 kb on + strand, within wecC at 4622.455 kb on - strand, within wecC at 4622.497 kb on + strand, within wecC at 4622.599 kb on - strand, within wecC at 4622.750 kb on + strand, within wecC at 4623.094 kb on - strand at 4623.094 kb on - strand at 4623.094 kb on - strand at 4623.096 kb on + strand at 4623.096 kb on + strand at 4623.096 kb on + strand at 4623.096 kb on + strand at 4623.096 kb on + strand at 4623.217 kb on - strand at 4623.236 kb on + strand at 4623.237 kb on - strand at 4623.277 kb on + strand at 4623.285 kb on - strand at 4623.307 kb on + strand at 4623.308 kb on - strand at 4623.501 kb on + strand, within wecB at 4623.502 kb on - strand, within wecB at 4623.527 kb on - strand, within wecB at 4623.543 kb on - strand, within wecB at 4623.573 kb on - strand, within wecB at 4623.579 kb on - strand, within wecB at 4623.789 kb on + strand, within wecB at 4623.789 kb on + strand, within wecB at 4623.790 kb on - strand, within wecB at 4623.805 kb on - strand, within wecB at 4623.913 kb on + strand, within wecB at 4623.939 kb on - strand, within wecB at 4623.967 kb on + strand, within wecB at 4624.022 kb on - strand, within wecB at 4624.098 kb on - strand, within wecB at 4624.101 kb on + strand, within wecB at 4624.111 kb on - strand, within wecB at 4624.206 kb on + strand, within wecB at 4624.258 kb on - strand at 4624.595 kb on + strand, within wzzE at 4624.688 kb on - strand, within wzzE at 4624.705 kb on - strand, within wzzE at 4624.781 kb on + strand, within wzzE at 4624.782 kb on - strand, within wzzE at 4624.907 kb on + strand, within wzzE at 4625.002 kb on + strand, within wzzE at 4625.003 kb on - strand, within wzzE at 4625.027 kb on - strand, within wzzE at 4625.027 kb on - strand, within wzzE at 4625.032 kb on - strand, within wzzE at 4625.032 kb on - strand, within wzzE at 4625.032 kb on - strand, within wzzE at 4625.070 kb on - strand, within wzzE at 4625.073 kb on - strand, within wzzE at 4625.172 kb on + strand, within wzzE at 4625.172 kb on + strand, within wzzE at 4625.173 kb on - strand, within wzzE at 4625.173 kb on - strand, within wzzE at 4625.173 kb on - strand, within wzzE at 4625.173 kb on - strand, within wzzE at 4625.181 kb on + strand, within wzzE at 4625.200 kb on - strand, within wzzE at 4625.222 kb on + strand, within wzzE at 4625.238 kb on + strand, within wzzE at 4625.239 kb on - strand, within wzzE at 4625.307 kb on - strand, within wzzE at 4625.307 kb on - strand, within wzzE at 4625.324 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS) remove 4,622,271 + wecC LU632_RS24235 0.25 -0.3 4,622,397 + wecC LU632_RS24235 0.35 -2.4 4,622,455 - wecC LU632_RS24235 0.40 -0.2 4,622,497 + wecC LU632_RS24235 0.43 -0.9 4,622,599 - wecC LU632_RS24235 0.51 -2.5 4,622,750 + wecC LU632_RS24235 0.63 -1.3 4,623,094 - -1.4 4,623,094 - +1.0 4,623,094 - -0.5 4,623,096 + +1.9 4,623,096 + -0.5 4,623,096 + +0.1 4,623,096 + +0.9 4,623,096 + +0.3 4,623,217 - +2.0 4,623,236 + -1.8 4,623,237 - +1.4 4,623,277 + -3.3 4,623,285 - +1.1 4,623,307 + -1.0 4,623,308 - -1.9 4,623,501 + wecB LU632_RS24240 0.25 -0.1 4,623,502 - wecB LU632_RS24240 0.26 -1.0 4,623,527 - wecB LU632_RS24240 0.28 -1.8 4,623,543 - wecB LU632_RS24240 0.29 -0.5 4,623,573 - wecB LU632_RS24240 0.32 -0.7 4,623,579 - wecB LU632_RS24240 0.32 -1.1 4,623,789 + wecB LU632_RS24240 0.51 +0.0 4,623,789 + wecB LU632_RS24240 0.51 -2.9 4,623,790 - wecB LU632_RS24240 0.51 -2.3 4,623,805 - wecB LU632_RS24240 0.52 -1.4 4,623,913 + wecB LU632_RS24240 0.62 +0.1 4,623,939 - wecB LU632_RS24240 0.64 -2.5 4,623,967 + wecB LU632_RS24240 0.67 +1.7 4,624,022 - wecB LU632_RS24240 0.72 +0.2 4,624,098 - wecB LU632_RS24240 0.78 -0.1 4,624,101 + wecB LU632_RS24240 0.79 -0.9 4,624,111 - wecB LU632_RS24240 0.79 -2.4 4,624,206 + wecB LU632_RS24240 0.88 -2.7 4,624,258 - -2.2 4,624,595 + wzzE LU632_RS24245 0.20 +1.5 4,624,688 - wzzE LU632_RS24245 0.29 +0.1 4,624,705 - wzzE LU632_RS24245 0.30 +1.0 4,624,781 + wzzE LU632_RS24245 0.38 -0.7 4,624,782 - wzzE LU632_RS24245 0.38 +0.9 4,624,907 + wzzE LU632_RS24245 0.50 +0.1 4,625,002 + wzzE LU632_RS24245 0.59 -0.4 4,625,003 - wzzE LU632_RS24245 0.59 +0.9 4,625,027 - wzzE LU632_RS24245 0.62 -0.7 4,625,027 - wzzE LU632_RS24245 0.62 -1.7 4,625,032 - wzzE LU632_RS24245 0.62 -0.1 4,625,032 - wzzE LU632_RS24245 0.62 -1.1 4,625,032 - wzzE LU632_RS24245 0.62 -1.7 4,625,070 - wzzE LU632_RS24245 0.66 -0.1 4,625,073 - wzzE LU632_RS24245 0.66 -0.3 4,625,172 + wzzE LU632_RS24245 0.76 -2.3 4,625,172 + wzzE LU632_RS24245 0.76 -0.9 4,625,173 - wzzE LU632_RS24245 0.76 +1.3 4,625,173 - wzzE LU632_RS24245 0.76 +2.4 4,625,173 - wzzE LU632_RS24245 0.76 -3.1 4,625,173 - wzzE LU632_RS24245 0.76 +0.4 4,625,181 + wzzE LU632_RS24245 0.77 -1.3 4,625,200 - wzzE LU632_RS24245 0.78 -1.5 4,625,222 + wzzE LU632_RS24245 0.81 -0.1 4,625,238 + wzzE LU632_RS24245 0.82 -1.4 4,625,239 - wzzE LU632_RS24245 0.82 +0.3 4,625,307 - wzzE LU632_RS24245 0.89 +0.8 4,625,307 - wzzE LU632_RS24245 0.89 +0.9 4,625,324 - +0.9
Or see this region's nucleotide sequence