Experiment: Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnpA and LU632_RS18585 are separated by 529 nucleotides LU632_RS18585 and nrdR are separated by 251 nucleotides nrdR and ribD are separated by 3 nucleotides
LU632_RS18580: tnpA - IS200/IS605 family transposase, at 3,514,481 to 3,514,918
tnpA
LU632_RS18585: LU632_RS18585 - DUF3251 domain-containing protein, at 3,515,448 to 3,516,002
_RS18585
LU632_RS18590: nrdR - transcriptional regulator NrdR, at 3,516,254 to 3,516,703
nrdR
LU632_RS18595: ribD - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 3,516,707 to 3,517,810
ribD
Position (kb)
3515
3516
3517 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3515.006 kb on + strand at 3515.053 kb on + strand at 3515.202 kb on + strand at 3515.231 kb on + strand at 3515.425 kb on + strand at 3515.597 kb on + strand, within LU632_RS18585 at 3515.652 kb on - strand, within LU632_RS18585 at 3515.904 kb on - strand, within LU632_RS18585 at 3515.974 kb on - strand at 3516.114 kb on + strand at 3516.114 kb on + strand at 3516.115 kb on - strand at 3516.132 kb on + strand at 3516.189 kb on + strand at 3516.581 kb on - strand, within nrdR at 3516.623 kb on + strand, within nrdR at 3516.625 kb on + strand, within nrdR at 3516.706 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS) remove 3,515,006 + +1.6 3,515,053 + +1.5 3,515,202 + -1.3 3,515,231 + +0.0 3,515,425 + +1.7 3,515,597 + LU632_RS18585 0.27 -1.7 3,515,652 - LU632_RS18585 0.37 -3.2 3,515,904 - LU632_RS18585 0.82 +1.6 3,515,974 - -0.0 3,516,114 + +1.1 3,516,114 + +0.3 3,516,115 - +0.1 3,516,132 + +0.3 3,516,189 + +0.6 3,516,581 - nrdR LU632_RS18590 0.73 +0.6 3,516,623 + nrdR LU632_RS18590 0.82 -1.7 3,516,625 + nrdR LU632_RS18590 0.82 -0.5 3,516,706 + +0.6
Or see this region's nucleotide sequence