Experiment: Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS13835 and fliD are separated by 280 nucleotides fliD and fliS are separated by 13 nucleotides fliS and fliT are separated by 0 nucleotides fliT and amyA are separated by 133 nucleotides
LU632_RS13835: LU632_RS13835 - FliC/FljB family flagellin, at 2,654,944 to 2,656,227
_RS13835
LU632_RS13840: fliD - flagellar filament capping protein FliD, at 2,656,508 to 2,657,920
fliD
LU632_RS13845: fliS - flagellar export chaperone FliS, at 2,657,934 to 2,658,341
fliS
LU632_RS13850: fliT - flagella biosynthesis regulatory protein FliT, at 2,658,342 to 2,658,710
fliT
LU632_RS13855: amyA - alpha-amylase, at 2,658,844 to 2,659,646
amyA
Position (kb)
2656
2657
2658 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2655.534 kb on - strand, within LU632_RS13835 at 2655.534 kb on - strand, within LU632_RS13835 at 2655.662 kb on - strand, within LU632_RS13835 at 2655.662 kb on - strand, within LU632_RS13835 at 2655.962 kb on - strand, within LU632_RS13835 at 2655.967 kb on - strand, within LU632_RS13835 at 2655.967 kb on - strand, within LU632_RS13835 at 2656.015 kb on - strand, within LU632_RS13835 at 2656.230 kb on + strand at 2656.259 kb on + strand at 2656.388 kb on + strand at 2656.433 kb on + strand at 2656.433 kb on + strand at 2656.471 kb on + strand at 2656.471 kb on + strand at 2656.508 kb on - strand at 2656.518 kb on + strand at 2656.702 kb on + strand, within fliD at 2656.786 kb on + strand, within fliD at 2656.790 kb on + strand, within fliD at 2656.791 kb on - strand, within fliD at 2656.888 kb on + strand, within fliD at 2656.889 kb on - strand, within fliD at 2657.005 kb on + strand, within fliD at 2657.006 kb on - strand, within fliD at 2657.006 kb on - strand, within fliD at 2657.031 kb on - strand, within fliD at 2657.218 kb on + strand, within fliD at 2657.330 kb on - strand, within fliD at 2657.345 kb on + strand, within fliD at 2657.345 kb on + strand, within fliD at 2657.346 kb on - strand, within fliD at 2657.347 kb on + strand, within fliD at 2657.384 kb on + strand, within fliD at 2657.384 kb on + strand, within fliD at 2657.394 kb on + strand, within fliD at 2657.394 kb on + strand, within fliD at 2657.394 kb on + strand, within fliD at 2657.405 kb on - strand, within fliD at 2657.461 kb on + strand, within fliD at 2657.515 kb on + strand, within fliD at 2657.668 kb on + strand, within fliD at 2657.677 kb on + strand, within fliD at 2657.807 kb on + strand at 2657.863 kb on + strand at 2657.863 kb on + strand at 2657.937 kb on + strand at 2657.937 kb on + strand at 2658.198 kb on + strand, within fliS at 2658.339 kb on + strand at 2658.362 kb on - strand at 2658.533 kb on - strand, within fliT at 2658.648 kb on - strand, within fliT at 2658.657 kb on + strand, within fliT at 2658.658 kb on - strand, within fliT at 2658.659 kb on + strand, within fliT at 2658.763 kb on + strand at 2658.880 kb on + strand at 2658.880 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS) remove 2,655,534 - LU632_RS13835 0.46 -0.2 2,655,534 - LU632_RS13835 0.46 -0.5 2,655,662 - LU632_RS13835 0.56 +1.7 2,655,662 - LU632_RS13835 0.56 -0.7 2,655,962 - LU632_RS13835 0.79 +0.2 2,655,967 - LU632_RS13835 0.80 -2.6 2,655,967 - LU632_RS13835 0.80 -1.0 2,656,015 - LU632_RS13835 0.83 -0.6 2,656,230 + -0.6 2,656,259 + +2.5 2,656,388 + -1.6 2,656,433 + -0.2 2,656,433 + -0.0 2,656,471 + +0.7 2,656,471 + -0.6 2,656,508 - -0.9 2,656,518 + +1.1 2,656,702 + fliD LU632_RS13840 0.14 -1.6 2,656,786 + fliD LU632_RS13840 0.20 -0.6 2,656,790 + fliD LU632_RS13840 0.20 -1.1 2,656,791 - fliD LU632_RS13840 0.20 -1.6 2,656,888 + fliD LU632_RS13840 0.27 +0.0 2,656,889 - fliD LU632_RS13840 0.27 -3.2 2,657,005 + fliD LU632_RS13840 0.35 +0.5 2,657,006 - fliD LU632_RS13840 0.35 -1.2 2,657,006 - fliD LU632_RS13840 0.35 -1.3 2,657,031 - fliD LU632_RS13840 0.37 +1.2 2,657,218 + fliD LU632_RS13840 0.50 +0.1 2,657,330 - fliD LU632_RS13840 0.58 +0.4 2,657,345 + fliD LU632_RS13840 0.59 -0.8 2,657,345 + fliD LU632_RS13840 0.59 +0.0 2,657,346 - fliD LU632_RS13840 0.59 -1.6 2,657,347 + fliD LU632_RS13840 0.59 -1.3 2,657,384 + fliD LU632_RS13840 0.62 +0.4 2,657,384 + fliD LU632_RS13840 0.62 -0.5 2,657,394 + fliD LU632_RS13840 0.63 +3.9 2,657,394 + fliD LU632_RS13840 0.63 -0.2 2,657,394 + fliD LU632_RS13840 0.63 +0.4 2,657,405 - fliD LU632_RS13840 0.63 +0.5 2,657,461 + fliD LU632_RS13840 0.67 +1.2 2,657,515 + fliD LU632_RS13840 0.71 -0.6 2,657,668 + fliD LU632_RS13840 0.82 +0.1 2,657,677 + fliD LU632_RS13840 0.83 -2.9 2,657,807 + +1.0 2,657,863 + +0.2 2,657,863 + +0.3 2,657,937 + -0.1 2,657,937 + +1.1 2,658,198 + fliS LU632_RS13845 0.65 -1.2 2,658,339 + -1.6 2,658,362 - +2.9 2,658,533 - fliT LU632_RS13850 0.52 +1.9 2,658,648 - fliT LU632_RS13850 0.83 -0.9 2,658,657 + fliT LU632_RS13850 0.85 -1.4 2,658,658 - fliT LU632_RS13850 0.86 -3.8 2,658,659 + fliT LU632_RS13850 0.86 +0.8 2,658,763 + +0.4 2,658,880 + -0.2 2,658,880 + +3.2
Or see this region's nucleotide sequence