Experiment: Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnpA and LU632_RS11660 are separated by 220 nucleotides LU632_RS11660 and LU632_RS11665 overlap by 2 nucleotides LU632_RS11665 and rhaM are separated by 65 nucleotides rhaM and rhaD overlap by 11 nucleotides rhaD and LU632_RS11680 are separated by 9 nucleotides
LU632_RS11655: tnpA - IS200/IS605 family transposase, at 2,218,424 to 2,218,840
tnpA
LU632_RS11660: LU632_RS11660 - sugar transporter, at 2,219,061 to 2,219,201
_RS11660
LU632_RS11665: LU632_RS11665 - sugar transporter, at 2,219,200 to 2,219,429
_RS11665
LU632_RS11670: rhaM - L-rhamnose mutarotase, at 2,219,495 to 2,219,809
rhaM
LU632_RS11675: rhaD - rhamnulose-1-phosphate aldolase, at 2,219,799 to 2,220,641
rhaD
LU632_RS11680: LU632_RS11680 - L-rhamnose isomerase, at 2,220,651 to 2,221,907
_RS11680
Position (kb)
2219
2220
2221 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2218.948 kb on + strand at 2219.024 kb on + strand at 2219.050 kb on + strand at 2219.060 kb on + strand at 2219.060 kb on + strand at 2219.061 kb on - strand at 2219.061 kb on - strand at 2219.061 kb on - strand at 2219.061 kb on - strand at 2219.088 kb on - strand, within LU632_RS11660 at 2219.120 kb on - strand, within LU632_RS11660 at 2219.135 kb on - strand, within LU632_RS11660 at 2219.187 kb on + strand, within LU632_RS11660 at 2219.188 kb on - strand at 2219.191 kb on + strand at 2219.191 kb on + strand at 2219.191 kb on + strand at 2219.192 kb on - strand at 2219.207 kb on + strand at 2219.208 kb on - strand at 2219.208 kb on - strand at 2219.414 kb on - strand at 2219.414 kb on - strand at 2219.458 kb on + strand at 2219.539 kb on + strand, within rhaM at 2219.561 kb on - strand, within rhaM at 2219.561 kb on - strand, within rhaM at 2219.688 kb on + strand, within rhaM at 2219.757 kb on + strand, within rhaM at 2219.809 kb on + strand at 2219.809 kb on + strand at 2219.810 kb on - strand at 2219.810 kb on - strand at 2219.812 kb on - strand at 2219.891 kb on + strand, within rhaD at 2219.960 kb on + strand, within rhaD at 2220.188 kb on - strand, within rhaD at 2220.328 kb on + strand, within rhaD at 2220.328 kb on + strand, within rhaD at 2220.328 kb on + strand, within rhaD at 2220.331 kb on - strand, within rhaD at 2220.401 kb on + strand, within rhaD at 2220.584 kb on + strand at 2220.584 kb on + strand at 2220.584 kb on + strand at 2220.584 kb on + strand at 2220.584 kb on + strand at 2220.584 kb on + strand at 2220.585 kb on - strand at 2220.585 kb on - strand at 2220.679 kb on + strand at 2220.680 kb on - strand at 2220.688 kb on - strand at 2220.737 kb on - strand at 2220.803 kb on - strand, within LU632_RS11680 at 2220.849 kb on + strand, within LU632_RS11680 at 2220.850 kb on - strand, within LU632_RS11680 at 2220.850 kb on - strand, within LU632_RS11680 at 2220.906 kb on - strand, within LU632_RS11680 at 2221.056 kb on + strand, within LU632_RS11680 at 2221.072 kb on + strand, within LU632_RS11680 at 2221.072 kb on + strand, within LU632_RS11680 at 2221.072 kb on + strand, within LU632_RS11680 at 2221.072 kb on + strand, within LU632_RS11680 at 2221.072 kb on + strand, within LU632_RS11680 at 2221.199 kb on - strand, within LU632_RS11680 at 2221.214 kb on + strand, within LU632_RS11680 at 2221.244 kb on + strand, within LU632_RS11680 at 2221.275 kb on + strand, within LU632_RS11680 at 2221.296 kb on - strand, within LU632_RS11680 at 2221.296 kb on - strand, within LU632_RS11680 at 2221.330 kb on - strand, within LU632_RS11680
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS) remove 2,218,948 + -0.8 2,219,024 + +0.2 2,219,050 + +1.3 2,219,060 + -0.7 2,219,060 + -1.8 2,219,061 - -1.0 2,219,061 - +0.5 2,219,061 - -1.3 2,219,061 - -1.1 2,219,088 - LU632_RS11660 0.19 +0.6 2,219,120 - LU632_RS11660 0.42 +0.4 2,219,135 - LU632_RS11660 0.52 -1.3 2,219,187 + LU632_RS11660 0.89 +0.9 2,219,188 - -0.2 2,219,191 + -1.7 2,219,191 + -0.7 2,219,191 + -0.1 2,219,192 - +1.5 2,219,207 + -1.7 2,219,208 - -1.2 2,219,208 - -0.4 2,219,414 - +2.0 2,219,414 - -0.9 2,219,458 + +2.0 2,219,539 + rhaM LU632_RS11670 0.14 -2.3 2,219,561 - rhaM LU632_RS11670 0.21 +0.4 2,219,561 - rhaM LU632_RS11670 0.21 +0.6 2,219,688 + rhaM LU632_RS11670 0.61 -0.3 2,219,757 + rhaM LU632_RS11670 0.83 -1.7 2,219,809 + -1.7 2,219,809 + +1.7 2,219,810 - -0.1 2,219,810 - -3.7 2,219,812 - +0.6 2,219,891 + rhaD LU632_RS11675 0.11 -0.2 2,219,960 + rhaD LU632_RS11675 0.19 -1.5 2,220,188 - rhaD LU632_RS11675 0.46 -1.9 2,220,328 + rhaD LU632_RS11675 0.63 -1.9 2,220,328 + rhaD LU632_RS11675 0.63 -1.3 2,220,328 + rhaD LU632_RS11675 0.63 -2.7 2,220,331 - rhaD LU632_RS11675 0.63 -0.7 2,220,401 + rhaD LU632_RS11675 0.71 -0.8 2,220,584 + -1.6 2,220,584 + +0.3 2,220,584 + +1.6 2,220,584 + -2.0 2,220,584 + -0.1 2,220,584 + +0.4 2,220,585 - -0.0 2,220,585 - -1.0 2,220,679 + +1.1 2,220,680 - -2.6 2,220,688 - +0.4 2,220,737 - +0.3 2,220,803 - LU632_RS11680 0.12 -1.9 2,220,849 + LU632_RS11680 0.16 -3.0 2,220,850 - LU632_RS11680 0.16 +0.1 2,220,850 - LU632_RS11680 0.16 -2.0 2,220,906 - LU632_RS11680 0.20 -1.3 2,221,056 + LU632_RS11680 0.32 +0.2 2,221,072 + LU632_RS11680 0.33 -3.3 2,221,072 + LU632_RS11680 0.33 -0.1 2,221,072 + LU632_RS11680 0.33 +1.3 2,221,072 + LU632_RS11680 0.33 -0.6 2,221,072 + LU632_RS11680 0.33 +0.3 2,221,199 - LU632_RS11680 0.44 -1.4 2,221,214 + LU632_RS11680 0.45 -0.7 2,221,244 + LU632_RS11680 0.47 -1.9 2,221,275 + LU632_RS11680 0.50 -4.1 2,221,296 - LU632_RS11680 0.51 +0.9 2,221,296 - LU632_RS11680 0.51 +0.6 2,221,330 - LU632_RS11680 0.54 -1.5
Or see this region's nucleotide sequence