Experiment: Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and pgsA are separated by 71 nucleotides pgsA and LU632_RS10205 are separated by 148 nucleotides LU632_RS10205 and LU632_RS10210 are separated by 42 nucleotides LU632_RS10210 and LU632_RS10215 are separated by 12 nucleotides LU632_RS10215 and LU632_RS10220 are separated by 163 nucleotides
LU632_RS10195: uvrC - excinuclease ABC subunit UvrC, at 1,950,793 to 1,952,625
uvrC
LU632_RS10200: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 1,952,697 to 1,953,245
pgsA
LU632_RS10205: LU632_RS10205 - tRNA-Gly, at 1,953,394 to 1,953,469
_RS10205
LU632_RS10210: LU632_RS10210 - tRNA-Cys, at 1,953,512 to 1,953,585
_RS10210
LU632_RS10215: LU632_RS10215 - tRNA-Leu, at 1,953,598 to 1,953,683
_RS10215
LU632_RS10220: LU632_RS10220 - integrase arm-type DNA-binding domain-containing protein, at 1,953,847 to 1,954,824
_RS10220
Position (kb) 1952
1953
1954 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1951.852 kb on + strand, within uvrC at 1951.990 kb on - strand, within uvrC at 1951.992 kb on + strand, within uvrC at 1951.998 kb on + strand, within uvrC at 1951.999 kb on - strand, within uvrC at 1951.999 kb on - strand, within uvrC at 1951.999 kb on - strand, within uvrC at 1951.999 kb on - strand, within uvrC at 1952.141 kb on - strand, within uvrC at 1952.163 kb on + strand, within uvrC at 1952.164 kb on - strand, within uvrC at 1952.207 kb on + strand, within uvrC at 1952.208 kb on - strand, within uvrC at 1952.388 kb on + strand, within uvrC at 1952.511 kb on + strand at 1952.512 kb on - strand at 1952.516 kb on - strand at 1952.516 kb on - strand at 1952.608 kb on + strand at 1952.616 kb on - strand at 1952.649 kb on + strand at 1952.649 kb on + strand at 1952.657 kb on + strand at 1952.657 kb on + strand at 1953.211 kb on + strand at 1953.211 kb on + strand at 1953.211 kb on + strand at 1953.686 kb on - strand at 1953.686 kb on - strand at 1953.901 kb on - strand at 1953.901 kb on - strand at 1953.925 kb on + strand at 1953.955 kb on + strand, within LU632_RS10220 at 1954.017 kb on - strand, within LU632_RS10220 at 1954.050 kb on + strand, within LU632_RS10220 at 1954.138 kb on + strand, within LU632_RS10220 at 1954.139 kb on - strand, within LU632_RS10220 at 1954.139 kb on - strand, within LU632_RS10220 at 1954.177 kb on - strand, within LU632_RS10220 at 1954.177 kb on - strand, within LU632_RS10220 at 1954.181 kb on + strand, within LU632_RS10220 at 1954.182 kb on - strand, within LU632_RS10220 at 1954.233 kb on + strand, within LU632_RS10220 at 1954.234 kb on - strand, within LU632_RS10220
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS) remove 1,951,852 + uvrC LU632_RS10195 0.58 -2.4 1,951,990 - uvrC LU632_RS10195 0.65 -0.6 1,951,992 + uvrC LU632_RS10195 0.65 +0.4 1,951,998 + uvrC LU632_RS10195 0.66 -1.4 1,951,999 - uvrC LU632_RS10195 0.66 +0.4 1,951,999 - uvrC LU632_RS10195 0.66 -1.6 1,951,999 - uvrC LU632_RS10195 0.66 -2.8 1,951,999 - uvrC LU632_RS10195 0.66 +0.9 1,952,141 - uvrC LU632_RS10195 0.74 -1.9 1,952,163 + uvrC LU632_RS10195 0.75 -0.3 1,952,164 - uvrC LU632_RS10195 0.75 +0.4 1,952,207 + uvrC LU632_RS10195 0.77 +0.5 1,952,208 - uvrC LU632_RS10195 0.77 +0.2 1,952,388 + uvrC LU632_RS10195 0.87 -0.1 1,952,511 + -0.1 1,952,512 - -2.1 1,952,516 - +0.2 1,952,516 - +1.7 1,952,608 + -0.3 1,952,616 - -2.6 1,952,649 + -2.9 1,952,649 + -2.2 1,952,657 + -3.0 1,952,657 + +1.1 1,953,211 + -3.4 1,953,211 + +2.0 1,953,211 + -3.7 1,953,686 - -0.0 1,953,686 - +1.1 1,953,901 - +0.4 1,953,901 - +2.9 1,953,925 + -1.8 1,953,955 + LU632_RS10220 0.11 -1.9 1,954,017 - LU632_RS10220 0.17 +0.3 1,954,050 + LU632_RS10220 0.21 +0.8 1,954,138 + LU632_RS10220 0.30 -1.8 1,954,139 - LU632_RS10220 0.30 +0.8 1,954,139 - LU632_RS10220 0.30 +0.1 1,954,177 - LU632_RS10220 0.34 +1.0 1,954,177 - LU632_RS10220 0.34 +3.1 1,954,181 + LU632_RS10220 0.34 +1.3 1,954,182 - LU632_RS10220 0.34 -2.2 1,954,233 + LU632_RS10220 0.39 -0.4 1,954,234 - LU632_RS10220 0.40 -0.6
Or see this region's nucleotide sequence