Experiment: Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apbC and LU632_RS08580 are separated by 33 nucleotides LU632_RS08580 and LU632_RS08585 are separated by 29 nucleotides LU632_RS08585 and thiD are separated by 106 nucleotides thiD and LU632_RS08595 are separated by 41 nucleotides
LU632_RS08575: apbC - iron-sulfur cluster carrier protein ApbC, at 1,611,413 to 1,612,522
apbC
LU632_RS08580: LU632_RS08580 - hypothetical protein, at 1,612,556 to 1,612,714
_RS08580
LU632_RS08585: LU632_RS08585 - hydroxyethylthiazole kinase, at 1,612,744 to 1,613,361
_RS08585
LU632_RS08590: thiD - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 1,613,468 to 1,614,268
thiD
LU632_RS08595: LU632_RS08595 - hypothetical protein, at 1,614,310 to 1,614,471
_RS08595
Position (kb) 1612
1613
1614 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1611.871 kb on + strand, within apbC at 1611.871 kb on + strand, within apbC at 1611.965 kb on + strand, within apbC at 1611.989 kb on - strand, within apbC at 1611.989 kb on - strand, within apbC at 1611.989 kb on - strand, within apbC at 1612.109 kb on - strand, within apbC at 1612.124 kb on - strand, within apbC at 1612.124 kb on - strand, within apbC at 1612.219 kb on - strand, within apbC at 1612.224 kb on + strand, within apbC at 1612.243 kb on + strand, within apbC at 1612.244 kb on - strand, within apbC at 1612.257 kb on + strand, within apbC at 1612.258 kb on - strand, within apbC at 1612.258 kb on - strand, within apbC at 1612.258 kb on - strand, within apbC at 1612.557 kb on + strand at 1612.635 kb on + strand, within LU632_RS08580 at 1612.636 kb on - strand, within LU632_RS08580 at 1612.691 kb on - strand, within LU632_RS08580 at 1613.007 kb on - strand, within LU632_RS08585 at 1613.046 kb on + strand, within LU632_RS08585 at 1613.357 kb on + strand at 1613.372 kb on + strand at 1613.372 kb on + strand at 1613.372 kb on + strand at 1613.418 kb on - strand at 1613.418 kb on - strand at 1613.480 kb on - strand at 1614.076 kb on - strand, within thiD at 1614.165 kb on + strand, within thiD at 1614.166 kb on - strand, within thiD at 1614.166 kb on - strand, within thiD
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS) remove 1,611,871 + apbC LU632_RS08575 0.41 -0.4 1,611,871 + apbC LU632_RS08575 0.41 +2.9 1,611,965 + apbC LU632_RS08575 0.50 -2.5 1,611,989 - apbC LU632_RS08575 0.52 +0.4 1,611,989 - apbC LU632_RS08575 0.52 -2.8 1,611,989 - apbC LU632_RS08575 0.52 +1.5 1,612,109 - apbC LU632_RS08575 0.63 +1.9 1,612,124 - apbC LU632_RS08575 0.64 +1.4 1,612,124 - apbC LU632_RS08575 0.64 +0.6 1,612,219 - apbC LU632_RS08575 0.73 -1.4 1,612,224 + apbC LU632_RS08575 0.73 -1.2 1,612,243 + apbC LU632_RS08575 0.75 +0.1 1,612,244 - apbC LU632_RS08575 0.75 -1.7 1,612,257 + apbC LU632_RS08575 0.76 +0.2 1,612,258 - apbC LU632_RS08575 0.76 +0.2 1,612,258 - apbC LU632_RS08575 0.76 +4.4 1,612,258 - apbC LU632_RS08575 0.76 +0.9 1,612,557 + +0.1 1,612,635 + LU632_RS08580 0.50 +1.7 1,612,636 - LU632_RS08580 0.50 +0.6 1,612,691 - LU632_RS08580 0.85 +0.5 1,613,007 - LU632_RS08585 0.43 +0.1 1,613,046 + LU632_RS08585 0.49 -2.5 1,613,357 + +2.1 1,613,372 + +2.4 1,613,372 + +0.4 1,613,372 + +2.1 1,613,418 - -1.5 1,613,418 - -0.5 1,613,480 - -2.5 1,614,076 - thiD LU632_RS08590 0.76 +3.0 1,614,165 + thiD LU632_RS08590 0.87 +2.2 1,614,166 - thiD LU632_RS08590 0.87 -1.0 1,614,166 - thiD LU632_RS08590 0.87 -1.3
Or see this region's nucleotide sequence