Experiment: Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutY and trmB are separated by 151 nucleotides trmB and LU632_RS05430 overlap by 1 nucleotides LU632_RS05430 and LU632_RS05435 are separated by 65 nucleotides
LU632_RS05420: mutY - A/G-specific adenine glycosylase, at 1,047,188 to 1,048,339
mutY
LU632_RS05425: trmB - tRNA (guanosine(46)-N7)-methyltransferase TrmB, at 1,048,491 to 1,049,210
trmB
LU632_RS05430: LU632_RS05430 - YggL family protein, at 1,049,210 to 1,049,536
_RS05430
LU632_RS05435: LU632_RS05435 - DUF2884 domain-containing protein, at 1,049,602 to 1,050,321
_RS05435
Position (kb)
1048
1049
1050 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1047.502 kb on + strand, within mutY at 1047.503 kb on - strand, within mutY at 1047.681 kb on + strand, within mutY at 1047.682 kb on - strand, within mutY at 1047.796 kb on + strand, within mutY at 1047.866 kb on - strand, within mutY at 1047.889 kb on + strand, within mutY at 1047.954 kb on - strand, within mutY at 1047.977 kb on - strand, within mutY at 1048.015 kb on - strand, within mutY at 1048.015 kb on - strand, within mutY at 1048.089 kb on + strand, within mutY at 1048.113 kb on + strand, within mutY at 1048.114 kb on - strand, within mutY at 1048.114 kb on - strand, within mutY at 1048.115 kb on + strand, within mutY at 1048.115 kb on + strand, within mutY at 1048.115 kb on + strand, within mutY at 1048.115 kb on + strand, within mutY at 1048.115 kb on + strand, within mutY at 1048.115 kb on + strand, within mutY at 1048.115 kb on + strand, within mutY at 1048.115 kb on + strand, within mutY at 1048.116 kb on - strand, within mutY at 1048.116 kb on - strand, within mutY at 1048.116 kb on - strand, within mutY at 1048.153 kb on + strand, within mutY at 1048.154 kb on - strand, within mutY at 1048.154 kb on - strand, within mutY at 1048.154 kb on - strand, within mutY at 1048.172 kb on - strand, within mutY at 1048.243 kb on + strand at 1048.244 kb on - strand at 1048.244 kb on - strand at 1048.297 kb on - strand at 1048.347 kb on + strand at 1048.403 kb on + strand at 1048.448 kb on + strand at 1048.486 kb on + strand at 1048.868 kb on + strand, within trmB at 1048.874 kb on + strand, within trmB at 1048.874 kb on + strand, within trmB at 1048.875 kb on - strand, within trmB at 1048.875 kb on - strand, within trmB at 1048.967 kb on - strand, within trmB at 1049.051 kb on + strand, within trmB at 1049.051 kb on + strand, within trmB at 1049.051 kb on + strand, within trmB at 1049.052 kb on - strand, within trmB at 1049.052 kb on - strand, within trmB at 1049.052 kb on - strand, within trmB at 1049.052 kb on - strand, within trmB at 1049.052 kb on - strand, within trmB at 1049.073 kb on - strand, within trmB at 1049.109 kb on + strand, within trmB at 1049.109 kb on + strand, within trmB at 1049.110 kb on - strand, within trmB at 1049.110 kb on - strand, within trmB at 1049.110 kb on - strand, within trmB at 1049.110 kb on - strand, within trmB at 1049.113 kb on + strand, within trmB at 1049.535 kb on - strand at 1049.607 kb on + strand at 1049.607 kb on + strand at 1049.630 kb on + strand at 1049.662 kb on + strand at 1049.714 kb on + strand, within LU632_RS05435 at 1049.714 kb on + strand, within LU632_RS05435 at 1049.715 kb on - strand, within LU632_RS05435 at 1049.998 kb on - strand, within LU632_RS05435 at 1050.044 kb on + strand, within LU632_RS05435 at 1050.045 kb on - strand, within LU632_RS05435 at 1050.084 kb on + strand, within LU632_RS05435 at 1050.085 kb on - strand, within LU632_RS05435
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS) remove 1,047,502 + mutY LU632_RS05420 0.27 -2.6 1,047,503 - mutY LU632_RS05420 0.27 -0.3 1,047,681 + mutY LU632_RS05420 0.43 -0.4 1,047,682 - mutY LU632_RS05420 0.43 +1.2 1,047,796 + mutY LU632_RS05420 0.53 +0.2 1,047,866 - mutY LU632_RS05420 0.59 -0.5 1,047,889 + mutY LU632_RS05420 0.61 -1.7 1,047,954 - mutY LU632_RS05420 0.66 +0.7 1,047,977 - mutY LU632_RS05420 0.68 -1.1 1,048,015 - mutY LU632_RS05420 0.72 +0.1 1,048,015 - mutY LU632_RS05420 0.72 -0.4 1,048,089 + mutY LU632_RS05420 0.78 +2.2 1,048,113 + mutY LU632_RS05420 0.80 +0.2 1,048,114 - mutY LU632_RS05420 0.80 +0.6 1,048,114 - mutY LU632_RS05420 0.80 -0.6 1,048,115 + mutY LU632_RS05420 0.80 +0.2 1,048,115 + mutY LU632_RS05420 0.80 -1.5 1,048,115 + mutY LU632_RS05420 0.80 +2.3 1,048,115 + mutY LU632_RS05420 0.80 +0.6 1,048,115 + mutY LU632_RS05420 0.80 +2.9 1,048,115 + mutY LU632_RS05420 0.80 +0.9 1,048,115 + mutY LU632_RS05420 0.80 +0.6 1,048,115 + mutY LU632_RS05420 0.80 -0.9 1,048,116 - mutY LU632_RS05420 0.81 -1.2 1,048,116 - mutY LU632_RS05420 0.81 +2.7 1,048,116 - mutY LU632_RS05420 0.81 +1.7 1,048,153 + mutY LU632_RS05420 0.84 +1.8 1,048,154 - mutY LU632_RS05420 0.84 +0.2 1,048,154 - mutY LU632_RS05420 0.84 -0.4 1,048,154 - mutY LU632_RS05420 0.84 +0.8 1,048,172 - mutY LU632_RS05420 0.85 -0.5 1,048,243 + +0.4 1,048,244 - +0.8 1,048,244 - -1.1 1,048,297 - -2.3 1,048,347 + +0.6 1,048,403 + -0.5 1,048,448 + +1.2 1,048,486 + +2.0 1,048,868 + trmB LU632_RS05425 0.52 +0.2 1,048,874 + trmB LU632_RS05425 0.53 -1.4 1,048,874 + trmB LU632_RS05425 0.53 +3.4 1,048,875 - trmB LU632_RS05425 0.53 +0.4 1,048,875 - trmB LU632_RS05425 0.53 +0.2 1,048,967 - trmB LU632_RS05425 0.66 +0.2 1,049,051 + trmB LU632_RS05425 0.78 -1.4 1,049,051 + trmB LU632_RS05425 0.78 -0.5 1,049,051 + trmB LU632_RS05425 0.78 -0.4 1,049,052 - trmB LU632_RS05425 0.78 -0.5 1,049,052 - trmB LU632_RS05425 0.78 -2.0 1,049,052 - trmB LU632_RS05425 0.78 -0.4 1,049,052 - trmB LU632_RS05425 0.78 -3.1 1,049,052 - trmB LU632_RS05425 0.78 -0.4 1,049,073 - trmB LU632_RS05425 0.81 -2.4 1,049,109 + trmB LU632_RS05425 0.86 -0.2 1,049,109 + trmB LU632_RS05425 0.86 -0.8 1,049,110 - trmB LU632_RS05425 0.86 -0.4 1,049,110 - trmB LU632_RS05425 0.86 +0.6 1,049,110 - trmB LU632_RS05425 0.86 -0.4 1,049,110 - trmB LU632_RS05425 0.86 -1.5 1,049,113 + trmB LU632_RS05425 0.86 -0.7 1,049,535 - -1.9 1,049,607 + -3.1 1,049,607 + -0.6 1,049,630 + +0.2 1,049,662 + +0.4 1,049,714 + LU632_RS05435 0.16 -1.2 1,049,714 + LU632_RS05435 0.16 +2.8 1,049,715 - LU632_RS05435 0.16 +0.7 1,049,998 - LU632_RS05435 0.55 -0.1 1,050,044 + LU632_RS05435 0.61 -1.7 1,050,045 - LU632_RS05435 0.62 -2.5 1,050,084 + LU632_RS05435 0.67 +0.2 1,050,085 - LU632_RS05435 0.67 -2.2
Or see this region's nucleotide sequence