Experiment: Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt radC and LU632_RS04125 are separated by 12 nucleotides LU632_RS04125 and LU632_RS04130 are separated by 22 nucleotides LU632_RS04130 and LU632_RS04135 are separated by 52 nucleotides LU632_RS04135 and LU632_RS04140 overlap by 7 nucleotides LU632_RS04140 and istA are separated by 231 nucleotides
LU632_RS04120: radC - DNA repair protein RadC, at 819,137 to 819,616
radC
LU632_RS04125: LU632_RS04125 - DUF987 domain-containing protein, at 819,629 to 819,850
_RS04125
LU632_RS04130: LU632_RS04130 - type IV toxin-antitoxin system YeeU family antitoxin, at 819,873 to 820,232
_RS04130
LU632_RS04135: LU632_RS04135 - toxin, at 820,285 to 820,461
_RS04135
LU632_RS04140: LU632_RS04140 - terminase family protein, at 820,455 to 820,766
_RS04140
LU632_RS04145: istA - IS21 family transposase, at 820,998 to 822,527
istA
Position (kb)
820
821 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 819.352 kb on + strand, within radC at 819.353 kb on - strand, within radC at 819.624 kb on + strand at 819.663 kb on + strand, within LU632_RS04125 at 819.664 kb on - strand, within LU632_RS04125 at 819.713 kb on + strand, within LU632_RS04125 at 819.818 kb on + strand, within LU632_RS04125 at 819.819 kb on - strand, within LU632_RS04125 at 819.819 kb on - strand, within LU632_RS04125 at 819.819 kb on - strand, within LU632_RS04125 at 819.819 kb on - strand, within LU632_RS04125 at 819.849 kb on - strand at 819.854 kb on - strand at 819.869 kb on + strand at 819.890 kb on + strand at 819.990 kb on + strand, within LU632_RS04130 at 819.991 kb on - strand, within LU632_RS04130 at 820.010 kb on + strand, within LU632_RS04130 at 820.010 kb on + strand, within LU632_RS04130 at 820.011 kb on - strand, within LU632_RS04130 at 820.011 kb on - strand, within LU632_RS04130 at 820.140 kb on + strand, within LU632_RS04130 at 820.140 kb on + strand, within LU632_RS04130 at 820.140 kb on + strand, within LU632_RS04130 at 820.141 kb on - strand, within LU632_RS04130 at 820.191 kb on - strand, within LU632_RS04130 at 820.194 kb on + strand, within LU632_RS04130 at 820.195 kb on - strand, within LU632_RS04130 at 820.253 kb on + strand at 820.254 kb on - strand at 820.292 kb on + strand at 820.292 kb on + strand at 820.354 kb on - strand, within LU632_RS04135 at 820.375 kb on - strand, within LU632_RS04135 at 820.446 kb on + strand at 820.455 kb on - strand at 820.496 kb on + strand, within LU632_RS04140 at 820.694 kb on - strand, within LU632_RS04140
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS) remove 819,352 + radC LU632_RS04120 0.45 -1.0 819,353 - radC LU632_RS04120 0.45 -1.7 819,624 + -0.1 819,663 + LU632_RS04125 0.15 +0.3 819,664 - LU632_RS04125 0.16 +1.2 819,713 + LU632_RS04125 0.38 +0.6 819,818 + LU632_RS04125 0.85 -1.4 819,819 - LU632_RS04125 0.86 +2.2 819,819 - LU632_RS04125 0.86 +0.6 819,819 - LU632_RS04125 0.86 +0.0 819,819 - LU632_RS04125 0.86 +2.0 819,849 - -2.4 819,854 - -1.7 819,869 + +2.8 819,890 + -0.3 819,990 + LU632_RS04130 0.33 +1.0 819,991 - LU632_RS04130 0.33 -2.9 820,010 + LU632_RS04130 0.38 -1.0 820,010 + LU632_RS04130 0.38 +1.0 820,011 - LU632_RS04130 0.38 +0.1 820,011 - LU632_RS04130 0.38 +2.1 820,140 + LU632_RS04130 0.74 +1.6 820,140 + LU632_RS04130 0.74 +0.6 820,140 + LU632_RS04130 0.74 -2.6 820,141 - LU632_RS04130 0.74 -0.6 820,191 - LU632_RS04130 0.88 +1.1 820,194 + LU632_RS04130 0.89 +0.6 820,195 - LU632_RS04130 0.89 +1.2 820,253 + +1.9 820,254 - +2.0 820,292 + -0.5 820,292 + +0.6 820,354 - LU632_RS04135 0.39 +1.3 820,375 - LU632_RS04135 0.51 -1.1 820,446 + +0.6 820,455 - +2.2 820,496 + LU632_RS04140 0.13 -0.1 820,694 - LU632_RS04140 0.77 +1.6
Or see this region's nucleotide sequence