Experiment: Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sctC and LU632_RS03245 are separated by 14 nucleotides LU632_RS03245 and LU632_RS03250 overlap by 8 nucleotides LU632_RS03250 and LU632_RS03255 are separated by 189 nucleotides
LU632_RS03240: sctC - type III secretion system outer membrane ring subunit SctC, at 660,144 to 662,168
sctC
LU632_RS03245: LU632_RS03245 - hypothetical protein, at 662,183 to 662,377
_RS03245
LU632_RS03250: LU632_RS03250 - type III secretion system protein, at 662,370 to 662,720
_RS03250
LU632_RS03255: LU632_RS03255 - harpin HrpZ family protein, at 662,910 to 664,127
_RS03255
Position (kb) 662
663 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 661.200 kb on - strand, within sctC at 661.250 kb on + strand, within sctC at 661.251 kb on - strand, within sctC at 661.251 kb on - strand, within sctC at 661.320 kb on + strand, within sctC at 661.321 kb on - strand, within sctC at 661.326 kb on - strand, within sctC at 661.442 kb on + strand, within sctC at 661.442 kb on + strand, within sctC at 661.610 kb on + strand, within sctC at 661.626 kb on - strand, within sctC at 661.723 kb on - strand, within sctC at 661.736 kb on + strand, within sctC at 661.750 kb on + strand, within sctC at 661.793 kb on + strand, within sctC at 661.793 kb on + strand, within sctC at 661.794 kb on - strand, within sctC at 661.796 kb on + strand, within sctC at 661.797 kb on - strand, within sctC at 661.918 kb on + strand, within sctC at 661.918 kb on + strand, within sctC at 661.919 kb on - strand, within sctC at 661.919 kb on - strand, within sctC at 661.919 kb on - strand, within sctC at 661.945 kb on - strand, within sctC at 662.049 kb on - strand at 662.049 kb on - strand at 662.075 kb on + strand at 662.106 kb on + strand at 662.107 kb on - strand at 662.107 kb on - strand at 662.107 kb on - strand at 662.108 kb on + strand at 662.109 kb on - strand at 662.109 kb on - strand at 662.303 kb on + strand, within LU632_RS03245 at 662.303 kb on + strand, within LU632_RS03245 at 662.375 kb on + strand at 662.483 kb on + strand, within LU632_RS03250 at 662.557 kb on + strand, within LU632_RS03250 at 662.586 kb on + strand, within LU632_RS03250 at 662.606 kb on + strand, within LU632_RS03250 at 662.890 kb on - strand at 662.905 kb on - strand at 662.909 kb on + strand at 662.924 kb on + strand at 662.942 kb on + strand at 662.943 kb on - strand at 662.975 kb on + strand at 662.975 kb on + strand at 662.976 kb on - strand at 662.976 kb on - strand at 662.979 kb on - strand at 662.979 kb on - strand at 662.979 kb on - strand at 663.017 kb on + strand at 663.017 kb on + strand at 663.018 kb on - strand at 663.059 kb on + strand, within LU632_RS03255 at 663.059 kb on + strand, within LU632_RS03255 at 663.065 kb on + strand, within LU632_RS03255 at 663.247 kb on + strand, within LU632_RS03255 at 663.248 kb on - strand, within LU632_RS03255 at 663.256 kb on + strand, within LU632_RS03255 at 663.256 kb on + strand, within LU632_RS03255 at 663.328 kb on + strand, within LU632_RS03255
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Main Stem (TE2-S-C5-MS) remove 661,200 - sctC LU632_RS03240 0.52 -1.4 661,250 + sctC LU632_RS03240 0.55 -1.5 661,251 - sctC LU632_RS03240 0.55 +1.3 661,251 - sctC LU632_RS03240 0.55 -1.3 661,320 + sctC LU632_RS03240 0.58 -1.8 661,321 - sctC LU632_RS03240 0.58 -0.1 661,326 - sctC LU632_RS03240 0.58 +4.1 661,442 + sctC LU632_RS03240 0.64 +1.9 661,442 + sctC LU632_RS03240 0.64 -1.3 661,610 + sctC LU632_RS03240 0.72 -4.7 661,626 - sctC LU632_RS03240 0.73 +1.7 661,723 - sctC LU632_RS03240 0.78 +0.7 661,736 + sctC LU632_RS03240 0.79 -1.1 661,750 + sctC LU632_RS03240 0.79 -2.4 661,793 + sctC LU632_RS03240 0.81 +0.7 661,793 + sctC LU632_RS03240 0.81 +0.8 661,794 - sctC LU632_RS03240 0.81 +0.3 661,796 + sctC LU632_RS03240 0.82 +1.0 661,797 - sctC LU632_RS03240 0.82 +0.8 661,918 + sctC LU632_RS03240 0.88 -2.8 661,918 + sctC LU632_RS03240 0.88 -2.6 661,919 - sctC LU632_RS03240 0.88 +1.3 661,919 - sctC LU632_RS03240 0.88 -1.1 661,919 - sctC LU632_RS03240 0.88 -0.3 661,945 - sctC LU632_RS03240 0.89 -0.4 662,049 - +0.2 662,049 - -3.1 662,075 + +2.4 662,106 + -0.2 662,107 - -0.5 662,107 - -1.5 662,107 - -1.8 662,108 + -0.8 662,109 - -1.3 662,109 - -1.6 662,303 + LU632_RS03245 0.62 +0.0 662,303 + LU632_RS03245 0.62 +0.6 662,375 + +2.4 662,483 + LU632_RS03250 0.32 -0.2 662,557 + LU632_RS03250 0.53 -0.1 662,586 + LU632_RS03250 0.62 +0.7 662,606 + LU632_RS03250 0.67 -2.4 662,890 - +2.7 662,905 - +1.1 662,909 + +2.2 662,924 + +0.3 662,942 + +0.7 662,943 - -0.6 662,975 + +1.1 662,975 + -3.1 662,976 - -0.8 662,976 - +0.9 662,979 - +2.1 662,979 - +1.2 662,979 - +1.8 663,017 + -0.3 663,017 + +0.9 663,018 - +0.7 663,059 + LU632_RS03255 0.12 +3.5 663,059 + LU632_RS03255 0.12 -3.0 663,065 + LU632_RS03255 0.13 -1.6 663,247 + LU632_RS03255 0.28 -0.3 663,248 - LU632_RS03255 0.28 +0.7 663,256 + LU632_RS03255 0.28 -1.9 663,256 + LU632_RS03255 0.28 +0.6 663,328 + LU632_RS03255 0.34 +0.2
Or see this region's nucleotide sequence