Experiment: Experiment:TE2, Cucumber 5 Petiole 1 (TE2-S-C5-P1)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recG and trmH overlap by 4 nucleotides trmH and spoT are separated by 3 nucleotides spoT and rpoZ are separated by 22 nucleotides rpoZ and gmk are separated by 53 nucleotides
LU632_RS22190: recG - ATP-dependent DNA helicase RecG, at 4,241,105 to 4,243,186
recG
LU632_RS22195: trmH - tRNA (guanosine(18)-2'-O)-methyltransferase TrmH, at 4,243,183 to 4,243,881
trmH
LU632_RS22200: spoT - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 4,243,885 to 4,245,999
spoT
LU632_RS22205: rpoZ - DNA-directed RNA polymerase subunit omega, at 4,246,022 to 4,246,297
rpoZ
LU632_RS22210: gmk - guanylate kinase, at 4,246,351 to 4,246,974
gmk
Position (kb)
4243
4244
4245
4246 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4242.948 kb on - strand, within recG at 4243.033 kb on + strand at 4243.034 kb on - strand at 4243.034 kb on - strand at 4243.035 kb on + strand at 4243.035 kb on + strand at 4243.036 kb on - strand at 4243.036 kb on - strand at 4243.036 kb on - strand at 4243.093 kb on + strand at 4243.093 kb on + strand at 4243.094 kb on - strand at 4243.097 kb on + strand at 4243.098 kb on - strand at 4243.370 kb on + strand, within trmH at 4243.370 kb on + strand, within trmH at 4243.370 kb on + strand, within trmH at 4243.374 kb on + strand, within trmH at 4243.415 kb on - strand, within trmH at 4243.467 kb on + strand, within trmH at 4243.551 kb on + strand, within trmH at 4243.554 kb on - strand, within trmH at 4243.624 kb on - strand, within trmH at 4243.663 kb on + strand, within trmH at 4243.788 kb on + strand, within trmH at 4243.886 kb on + strand at 4243.896 kb on + strand at 4243.896 kb on + strand at 4243.943 kb on - strand at 4243.995 kb on - strand at 4244.098 kb on - strand, within spoT at 4244.098 kb on - strand, within spoT at 4244.251 kb on - strand, within spoT at 4244.293 kb on + strand, within spoT at 4244.432 kb on - strand, within spoT at 4245.996 kb on - strand at 4246.020 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Petiole 1 (TE2-S-C5-P1) remove 4,242,948 - recG LU632_RS22190 0.89 -0.8 4,243,033 + -0.4 4,243,034 - -2.7 4,243,034 - +0.2 4,243,035 + +4.2 4,243,035 + +1.8 4,243,036 - +0.5 4,243,036 - -0.8 4,243,036 - -0.1 4,243,093 + +0.9 4,243,093 + +2.2 4,243,094 - -0.2 4,243,097 + -2.1 4,243,098 - -0.1 4,243,370 + trmH LU632_RS22195 0.27 -1.7 4,243,370 + trmH LU632_RS22195 0.27 +0.3 4,243,370 + trmH LU632_RS22195 0.27 -1.6 4,243,374 + trmH LU632_RS22195 0.27 +0.1 4,243,415 - trmH LU632_RS22195 0.33 -1.5 4,243,467 + trmH LU632_RS22195 0.41 -2.3 4,243,551 + trmH LU632_RS22195 0.53 -2.3 4,243,554 - trmH LU632_RS22195 0.53 +0.4 4,243,624 - trmH LU632_RS22195 0.63 -0.4 4,243,663 + trmH LU632_RS22195 0.69 -0.2 4,243,788 + trmH LU632_RS22195 0.87 +2.4 4,243,886 + -2.5 4,243,896 + +0.9 4,243,896 + +2.9 4,243,943 - -1.2 4,243,995 - +0.8 4,244,098 - spoT LU632_RS22200 0.10 +0.9 4,244,098 - spoT LU632_RS22200 0.10 +1.9 4,244,251 - spoT LU632_RS22200 0.17 -0.8 4,244,293 + spoT LU632_RS22200 0.19 +1.2 4,244,432 - spoT LU632_RS22200 0.26 -1.1 4,245,996 - +0.9 4,246,020 - -0.4
Or see this region's nucleotide sequence