Strain Fitness in Erwinia tracheiphila SCR3 around LU632_RS22200

Experiment: Experiment:TE2, Cucumber 5 Petiole 1 (TE2-S-C5-P1)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecG and trmH overlap by 4 nucleotidestrmH and spoT are separated by 3 nucleotidesspoT and rpoZ are separated by 22 nucleotidesrpoZ and gmk are separated by 53 nucleotides LU632_RS22190: recG - ATP-dependent DNA helicase RecG, at 4,241,105 to 4,243,186 recG LU632_RS22195: trmH - tRNA (guanosine(18)-2'-O)-methyltransferase TrmH, at 4,243,183 to 4,243,881 trmH LU632_RS22200: spoT - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 4,243,885 to 4,245,999 spoT LU632_RS22205: rpoZ - DNA-directed RNA polymerase subunit omega, at 4,246,022 to 4,246,297 rpoZ LU632_RS22210: gmk - guanylate kinase, at 4,246,351 to 4,246,974 gmk Position (kb) 4243 4244 4245 4246Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 4242.948 kb on - strand, within recGat 4243.033 kb on + strandat 4243.034 kb on - strandat 4243.034 kb on - strandat 4243.035 kb on + strandat 4243.035 kb on + strandat 4243.036 kb on - strandat 4243.036 kb on - strandat 4243.036 kb on - strandat 4243.093 kb on + strandat 4243.093 kb on + strandat 4243.094 kb on - strandat 4243.097 kb on + strandat 4243.098 kb on - strandat 4243.370 kb on + strand, within trmHat 4243.370 kb on + strand, within trmHat 4243.370 kb on + strand, within trmHat 4243.374 kb on + strand, within trmHat 4243.415 kb on - strand, within trmHat 4243.467 kb on + strand, within trmHat 4243.551 kb on + strand, within trmHat 4243.554 kb on - strand, within trmHat 4243.624 kb on - strand, within trmHat 4243.663 kb on + strand, within trmHat 4243.788 kb on + strand, within trmHat 4243.886 kb on + strandat 4243.896 kb on + strandat 4243.896 kb on + strandat 4243.943 kb on - strandat 4243.995 kb on - strandat 4244.098 kb on - strand, within spoTat 4244.098 kb on - strand, within spoTat 4244.251 kb on - strand, within spoTat 4244.293 kb on + strand, within spoTat 4244.432 kb on - strand, within spoTat 4245.996 kb on - strandat 4246.020 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Petiole 1 (TE2-S-C5-P1)
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4,242,948 - recG LU632_RS22190 0.89 -0.8
4,243,033 + -0.4
4,243,034 - -2.7
4,243,034 - +0.2
4,243,035 + +4.2
4,243,035 + +1.8
4,243,036 - +0.5
4,243,036 - -0.8
4,243,036 - -0.1
4,243,093 + +0.9
4,243,093 + +2.2
4,243,094 - -0.2
4,243,097 + -2.1
4,243,098 - -0.1
4,243,370 + trmH LU632_RS22195 0.27 -1.7
4,243,370 + trmH LU632_RS22195 0.27 +0.3
4,243,370 + trmH LU632_RS22195 0.27 -1.6
4,243,374 + trmH LU632_RS22195 0.27 +0.1
4,243,415 - trmH LU632_RS22195 0.33 -1.5
4,243,467 + trmH LU632_RS22195 0.41 -2.3
4,243,551 + trmH LU632_RS22195 0.53 -2.3
4,243,554 - trmH LU632_RS22195 0.53 +0.4
4,243,624 - trmH LU632_RS22195 0.63 -0.4
4,243,663 + trmH LU632_RS22195 0.69 -0.2
4,243,788 + trmH LU632_RS22195 0.87 +2.4
4,243,886 + -2.5
4,243,896 + +0.9
4,243,896 + +2.9
4,243,943 - -1.2
4,243,995 - +0.8
4,244,098 - spoT LU632_RS22200 0.10 +0.9
4,244,098 - spoT LU632_RS22200 0.10 +1.9
4,244,251 - spoT LU632_RS22200 0.17 -0.8
4,244,293 + spoT LU632_RS22200 0.19 +1.2
4,244,432 - spoT LU632_RS22200 0.26 -1.1
4,245,996 - +0.9
4,246,020 - -0.4

Or see this region's nucleotide sequence