Experiment: Experiment:TE2, Cucumber 5 Petiole 1 (TE2-S-C5-P1)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pnp and rpsO are separated by 299 nucleotides rpsO and truB are separated by 109 nucleotides truB and rbfA overlap by 1 nucleotides rbfA and infB are separated by 152 nucleotides
LU632_RS21040: pnp - polyribonucleotide nucleotidyltransferase, at 4,020,873 to 4,023,002
pnp
LU632_RS21045: rpsO - 30S ribosomal protein S15, at 4,023,302 to 4,023,571
rpsO
LU632_RS21050: truB - tRNA pseudouridine(55) synthase TruB, at 4,023,681 to 4,024,625
truB
LU632_RS21055: rbfA - 30S ribosome-binding factor RbfA, at 4,024,625 to 4,025,026
rbfA
LU632_RS21060: infB - translation initiation factor IF-2, at 4,025,179 to 4,027,863
infB
Position (kb)
4023
4024
4025 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7 at 4023.163 kb on - strand at 4023.168 kb on - strand at 4023.168 kb on - strand at 4023.224 kb on + strand at 4023.339 kb on + strand, within rpsO at 4023.634 kb on - strand at 4023.678 kb on + strand at 4023.678 kb on + strand at 4023.678 kb on + strand at 4023.678 kb on + strand at 4023.679 kb on - strand at 4023.679 kb on - strand at 4023.683 kb on - strand at 4023.758 kb on + strand at 4023.759 kb on - strand at 4023.833 kb on + strand, within truB at 4023.834 kb on - strand, within truB at 4023.838 kb on + strand, within truB at 4023.838 kb on + strand, within truB at 4023.838 kb on + strand, within truB at 4023.839 kb on - strand, within truB at 4023.907 kb on - strand, within truB at 4023.918 kb on + strand, within truB at 4023.990 kb on - strand, within truB at 4024.004 kb on - strand, within truB at 4024.030 kb on + strand, within truB at 4024.058 kb on + strand, within truB at 4024.058 kb on + strand, within truB at 4024.059 kb on - strand, within truB at 4024.059 kb on - strand, within truB at 4024.059 kb on - strand, within truB at 4024.059 kb on - strand, within truB at 4024.059 kb on - strand, within truB at 4024.090 kb on + strand, within truB at 4024.090 kb on + strand, within truB at 4024.090 kb on + strand, within truB at 4024.090 kb on + strand, within truB at 4024.094 kb on + strand, within truB at 4024.094 kb on + strand, within truB at 4024.095 kb on - strand, within truB at 4024.194 kb on + strand, within truB at 4024.195 kb on - strand, within truB at 4024.216 kb on + strand, within truB at 4024.364 kb on + strand, within truB at 4024.424 kb on - strand, within truB at 4024.572 kb on - strand at 4024.636 kb on + strand at 4024.685 kb on - strand, within rbfA at 4024.685 kb on - strand, within rbfA at 4025.084 kb on - strand at 4025.129 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Petiole 1 (TE2-S-C5-P1) remove 4,023,163 - -0.6 4,023,168 - +2.2 4,023,168 - +0.2 4,023,224 + -1.4 4,023,339 + rpsO LU632_RS21045 0.14 +0.4 4,023,634 - +0.7 4,023,678 + -1.8 4,023,678 + +0.2 4,023,678 + +3.1 4,023,678 + +1.2 4,023,679 - +1.8 4,023,679 - +0.5 4,023,683 - -2.2 4,023,758 + +0.2 4,023,759 - -1.2 4,023,833 + truB LU632_RS21050 0.16 -0.8 4,023,834 - truB LU632_RS21050 0.16 +1.6 4,023,838 + truB LU632_RS21050 0.17 -0.3 4,023,838 + truB LU632_RS21050 0.17 +0.7 4,023,838 + truB LU632_RS21050 0.17 +1.9 4,023,839 - truB LU632_RS21050 0.17 +0.7 4,023,907 - truB LU632_RS21050 0.24 -0.5 4,023,918 + truB LU632_RS21050 0.25 -0.8 4,023,990 - truB LU632_RS21050 0.33 +3.3 4,024,004 - truB LU632_RS21050 0.34 +2.9 4,024,030 + truB LU632_RS21050 0.37 -0.2 4,024,058 + truB LU632_RS21050 0.40 +0.8 4,024,058 + truB LU632_RS21050 0.40 -1.4 4,024,059 - truB LU632_RS21050 0.40 +0.0 4,024,059 - truB LU632_RS21050 0.40 +0.8 4,024,059 - truB LU632_RS21050 0.40 +7.1 4,024,059 - truB LU632_RS21050 0.40 +1.3 4,024,059 - truB LU632_RS21050 0.40 -1.3 4,024,090 + truB LU632_RS21050 0.43 +0.3 4,024,090 + truB LU632_RS21050 0.43 +1.3 4,024,090 + truB LU632_RS21050 0.43 +1.9 4,024,090 + truB LU632_RS21050 0.43 +0.3 4,024,094 + truB LU632_RS21050 0.44 -0.8 4,024,094 + truB LU632_RS21050 0.44 +1.2 4,024,095 - truB LU632_RS21050 0.44 +0.6 4,024,194 + truB LU632_RS21050 0.54 -0.1 4,024,195 - truB LU632_RS21050 0.54 -0.8 4,024,216 + truB LU632_RS21050 0.57 +0.5 4,024,364 + truB LU632_RS21050 0.72 +4.6 4,024,424 - truB LU632_RS21050 0.79 +0.2 4,024,572 - -2.8 4,024,636 + +0.8 4,024,685 - rbfA LU632_RS21055 0.15 -0.7 4,024,685 - rbfA LU632_RS21055 0.15 +2.2 4,025,084 - +0.4 4,025,129 - +0.9
Or see this region's nucleotide sequence