Experiment: Experiment:TE2, Cucumber 5 Petiole 1 (TE2-S-C5-P1)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcnB and folK overlap by 4 nucleotides folK and panB are separated by 60 nucleotides panB and panC are separated by 15 nucleotides panC and panD are separated by 69 nucleotides
LU632_RS20090: pcnB - polynucleotide adenylyltransferase PcnB, at 3,827,039 to 3,828,454
pcnB
LU632_RS20095: folK - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,828,451 to 3,828,930
folK
LU632_RS20100: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,828,991 to 3,829,785
panB
LU632_RS20105: panC - pantoate--beta-alanine ligase, at 3,829,801 to 3,830,655
panC
LU632_RS20110: panD - aspartate 1-decarboxylase, at 3,830,725 to 3,831,105
panD
Position (kb)
3828
3829
3830 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 3828.216 kb on + strand, within pcnB at 3828.958 kb on + strand at 3828.958 kb on + strand at 3828.958 kb on + strand at 3828.959 kb on - strand at 3828.974 kb on + strand at 3829.063 kb on + strand at 3829.072 kb on - strand, within panB at 3829.189 kb on + strand, within panB at 3829.189 kb on + strand, within panB at 3829.259 kb on - strand, within panB at 3829.300 kb on - strand, within panB at 3829.317 kb on + strand, within panB at 3829.318 kb on - strand, within panB at 3829.536 kb on + strand, within panB at 3829.574 kb on + strand, within panB at 3829.575 kb on - strand, within panB at 3829.575 kb on - strand, within panB at 3829.575 kb on - strand, within panB at 3829.579 kb on - strand, within panB at 3829.729 kb on + strand at 3829.729 kb on + strand at 3829.730 kb on - strand at 3829.781 kb on - strand at 3829.781 kb on - strand at 3829.869 kb on + strand at 3829.912 kb on - strand, within panC at 3829.912 kb on - strand, within panC at 3829.957 kb on - strand, within panC at 3830.126 kb on + strand, within panC at 3830.257 kb on + strand, within panC at 3830.302 kb on + strand, within panC at 3830.375 kb on - strand, within panC at 3830.472 kb on + strand, within panC at 3830.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Petiole 1 (TE2-S-C5-P1) remove 3,828,216 + pcnB LU632_RS20090 0.83 +5.8 3,828,958 + -0.2 3,828,958 + +0.1 3,828,958 + -2.1 3,828,959 - -0.6 3,828,974 + +0.0 3,829,063 + +0.8 3,829,072 - panB LU632_RS20100 0.10 +1.1 3,829,189 + panB LU632_RS20100 0.25 +0.4 3,829,189 + panB LU632_RS20100 0.25 -0.9 3,829,259 - panB LU632_RS20100 0.34 -2.4 3,829,300 - panB LU632_RS20100 0.39 +2.9 3,829,317 + panB LU632_RS20100 0.41 +3.1 3,829,318 - panB LU632_RS20100 0.41 +3.0 3,829,536 + panB LU632_RS20100 0.69 +2.2 3,829,574 + panB LU632_RS20100 0.73 -0.2 3,829,575 - panB LU632_RS20100 0.73 +1.1 3,829,575 - panB LU632_RS20100 0.73 -0.6 3,829,575 - panB LU632_RS20100 0.73 +0.1 3,829,579 - panB LU632_RS20100 0.74 +2.6 3,829,729 + +1.2 3,829,729 + +1.7 3,829,730 - -0.1 3,829,781 - -1.7 3,829,781 - +3.9 3,829,869 + -2.1 3,829,912 - panC LU632_RS20105 0.13 +1.9 3,829,912 - panC LU632_RS20105 0.13 -1.0 3,829,957 - panC LU632_RS20105 0.18 -0.7 3,830,126 + panC LU632_RS20105 0.38 -3.1 3,830,257 + panC LU632_RS20105 0.53 -1.5 3,830,302 + panC LU632_RS20105 0.59 +0.3 3,830,375 - panC LU632_RS20105 0.67 -0.4 3,830,472 + panC LU632_RS20105 0.78 -2.2 3,830,629 - -1.2
Or see this region's nucleotide sequence