Experiment: Experiment:TE2, Cucumber 5 Petiole 1 (TE2-S-C5-P1)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysZ and zipA are separated by 216 nucleotides zipA and ligA are separated by 77 nucleotides
LU632_RS06630: cysZ - sulfate transporter CysZ, at 1,273,250 to 1,274,011
cysZ
LU632_RS06635: zipA - cell division protein ZipA, at 1,274,228 to 1,275,148
zipA
LU632_RS06640: ligA - NAD-dependent DNA ligase LigA, at 1,275,226 to 1,277,244
ligA
Position (kb)
1274
1275
1276 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1273.231 kb on + strand at 1273.242 kb on + strand at 1273.243 kb on - strand at 1273.245 kb on - strand at 1273.266 kb on - strand at 1273.271 kb on - strand at 1273.277 kb on - strand at 1273.396 kb on + strand, within cysZ at 1273.455 kb on + strand, within cysZ at 1273.537 kb on + strand, within cysZ at 1273.543 kb on - strand, within cysZ at 1273.544 kb on + strand, within cysZ at 1273.544 kb on + strand, within cysZ at 1273.575 kb on + strand, within cysZ at 1273.576 kb on - strand, within cysZ at 1273.671 kb on - strand, within cysZ at 1273.691 kb on + strand, within cysZ at 1273.792 kb on - strand, within cysZ at 1273.792 kb on - strand, within cysZ at 1273.997 kb on - strand at 1274.026 kb on - strand at 1274.029 kb on + strand at 1274.029 kb on + strand at 1274.030 kb on - strand at 1274.051 kb on + strand at 1274.052 kb on - strand at 1274.052 kb on - strand at 1274.063 kb on - strand at 1274.171 kb on + strand at 1274.207 kb on + strand at 1274.745 kb on + strand, within zipA at 1274.899 kb on + strand, within zipA at 1274.899 kb on + strand, within zipA at 1274.995 kb on + strand, within zipA at 1275.034 kb on + strand, within zipA at 1275.107 kb on + strand at 1275.167 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Petiole 1 (TE2-S-C5-P1) remove 1,273,231 + +2.6 1,273,242 + +1.2 1,273,243 - +0.7 1,273,245 - -1.3 1,273,266 - +0.3 1,273,271 - +2.2 1,273,277 - -0.7 1,273,396 + cysZ LU632_RS06630 0.19 +2.7 1,273,455 + cysZ LU632_RS06630 0.27 +1.1 1,273,537 + cysZ LU632_RS06630 0.38 +1.2 1,273,543 - cysZ LU632_RS06630 0.38 -0.4 1,273,544 + cysZ LU632_RS06630 0.39 -0.4 1,273,544 + cysZ LU632_RS06630 0.39 +2.3 1,273,575 + cysZ LU632_RS06630 0.43 +2.9 1,273,576 - cysZ LU632_RS06630 0.43 -0.1 1,273,671 - cysZ LU632_RS06630 0.55 +0.9 1,273,691 + cysZ LU632_RS06630 0.58 +1.5 1,273,792 - cysZ LU632_RS06630 0.71 -0.5 1,273,792 - cysZ LU632_RS06630 0.71 -1.9 1,273,997 - -0.7 1,274,026 - -0.0 1,274,029 + +1.2 1,274,029 + +0.3 1,274,030 - +1.1 1,274,051 + +0.0 1,274,052 - -0.7 1,274,052 - +0.1 1,274,063 - +1.0 1,274,171 + -3.0 1,274,207 + +0.3 1,274,745 + zipA LU632_RS06635 0.56 -1.1 1,274,899 + zipA LU632_RS06635 0.73 -0.1 1,274,899 + zipA LU632_RS06635 0.73 -3.5 1,274,995 + zipA LU632_RS06635 0.83 -2.4 1,275,034 + zipA LU632_RS06635 0.88 -2.4 1,275,107 + -2.4 1,275,167 + +1.5
Or see this region's nucleotide sequence