Experiment: Experiment:TE2, Cucumber 5 Petiole 1 (TE2-S-C5-P1)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and LU632_RS00950 overlap by 4 nucleotides LU632_RS00950 and thiS are separated by 2 nucleotides thiS and LU632_RS00960 overlap by 17 nucleotides LU632_RS00960 and thiE overlap by 11 nucleotides thiE and thiC overlap by 4 nucleotides
LU632_RS00945: thiH - 2-iminoacetate synthase ThiH, at 206,390 to 207,520
thiH
LU632_RS00950: LU632_RS00950 - thiazole synthase, at 207,517 to 208,293
_RS00950
LU632_RS00955: thiS - sulfur carrier protein ThiS, at 208,296 to 208,496
thiS
LU632_RS00960: LU632_RS00960 - HesA/MoeB/ThiF family protein, at 208,480 to 209,241
_RS00960
LU632_RS00965: thiE - thiamine phosphate synthase, at 209,231 to 209,878
thiE
LU632_RS00970: thiC - phosphomethylpyrimidine synthase ThiC, at 209,875 to 211,809
thiC
Position (kb)
208
209
210 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 207.811 kb on - strand, within LU632_RS00950 at 207.811 kb on - strand, within LU632_RS00950 at 207.832 kb on - strand, within LU632_RS00950 at 207.909 kb on - strand, within LU632_RS00950 at 207.916 kb on + strand, within LU632_RS00950 at 207.917 kb on - strand, within LU632_RS00950 at 207.917 kb on - strand, within LU632_RS00950 at 208.012 kb on - strand, within LU632_RS00950 at 208.012 kb on - strand, within LU632_RS00950 at 208.012 kb on - strand, within LU632_RS00950 at 208.092 kb on + strand, within LU632_RS00950 at 208.093 kb on - strand, within LU632_RS00950 at 208.099 kb on + strand, within LU632_RS00950 at 208.100 kb on - strand, within LU632_RS00950 at 208.100 kb on - strand, within LU632_RS00950 at 208.121 kb on - strand, within LU632_RS00950 at 208.121 kb on - strand, within LU632_RS00950 at 208.289 kb on - strand at 208.298 kb on - strand at 208.482 kb on - strand at 208.591 kb on - strand, within LU632_RS00960 at 208.657 kb on + strand, within LU632_RS00960 at 208.658 kb on - strand, within LU632_RS00960 at 208.892 kb on - strand, within LU632_RS00960 at 209.103 kb on - strand, within LU632_RS00960 at 209.176 kb on - strand at 209.212 kb on - strand at 209.432 kb on + strand, within thiE at 209.433 kb on - strand, within thiE at 209.433 kb on - strand, within thiE at 209.488 kb on + strand, within thiE at 209.489 kb on - strand, within thiE at 209.503 kb on + strand, within thiE at 209.504 kb on - strand, within thiE at 209.505 kb on + strand, within thiE at 209.505 kb on + strand, within thiE at 209.505 kb on + strand, within thiE at 209.506 kb on - strand, within thiE at 209.506 kb on - strand, within thiE at 209.609 kb on + strand, within thiE at 209.609 kb on + strand, within thiE at 209.630 kb on + strand, within thiE at 209.630 kb on + strand, within thiE at 209.631 kb on - strand, within thiE at 209.668 kb on - strand, within thiE at 209.668 kb on - strand, within thiE at 209.878 kb on + strand at 209.915 kb on + strand at 209.916 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 5 Petiole 1 (TE2-S-C5-P1) remove 207,811 - LU632_RS00950 0.38 +0.0 207,811 - LU632_RS00950 0.38 -0.7 207,832 - LU632_RS00950 0.41 +0.2 207,909 - LU632_RS00950 0.50 +0.3 207,916 + LU632_RS00950 0.51 -1.5 207,917 - LU632_RS00950 0.51 +1.2 207,917 - LU632_RS00950 0.51 -2.3 208,012 - LU632_RS00950 0.64 -2.1 208,012 - LU632_RS00950 0.64 +1.2 208,012 - LU632_RS00950 0.64 -0.3 208,092 + LU632_RS00950 0.74 +1.0 208,093 - LU632_RS00950 0.74 -1.3 208,099 + LU632_RS00950 0.75 +0.8 208,100 - LU632_RS00950 0.75 -0.6 208,100 - LU632_RS00950 0.75 +0.9 208,121 - LU632_RS00950 0.78 -2.0 208,121 - LU632_RS00950 0.78 +0.4 208,289 - +3.8 208,298 - +1.8 208,482 - +0.8 208,591 - LU632_RS00960 0.15 -1.6 208,657 + LU632_RS00960 0.23 +1.5 208,658 - LU632_RS00960 0.23 +0.8 208,892 - LU632_RS00960 0.54 -1.1 209,103 - LU632_RS00960 0.82 +2.7 209,176 - -0.1 209,212 - -0.8 209,432 + thiE LU632_RS00965 0.31 -0.1 209,433 - thiE LU632_RS00965 0.31 -0.3 209,433 - thiE LU632_RS00965 0.31 +1.6 209,488 + thiE LU632_RS00965 0.40 -0.4 209,489 - thiE LU632_RS00965 0.40 +0.9 209,503 + thiE LU632_RS00965 0.42 +0.8 209,504 - thiE LU632_RS00965 0.42 +0.1 209,505 + thiE LU632_RS00965 0.42 +2.0 209,505 + thiE LU632_RS00965 0.42 -1.8 209,505 + thiE LU632_RS00965 0.42 +0.5 209,506 - thiE LU632_RS00965 0.42 -0.7 209,506 - thiE LU632_RS00965 0.42 -0.3 209,609 + thiE LU632_RS00965 0.58 +1.8 209,609 + thiE LU632_RS00965 0.58 +0.3 209,630 + thiE LU632_RS00965 0.62 -0.6 209,630 + thiE LU632_RS00965 0.62 +0.9 209,631 - thiE LU632_RS00965 0.62 -1.1 209,668 - thiE LU632_RS00965 0.67 +1.2 209,668 - thiE LU632_RS00965 0.67 -1.6 209,878 + -1.1 209,915 + -1.3 209,916 - +1.7
Or see this region's nucleotide sequence