Experiment: Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspE and gspF overlap by 11 nucleotides gspF and gspG are separated by 22 nucleotides gspG and gspI are separated by 1 nucleotides gspI and gspJ overlap by 4 nucleotides
LU632_RS25235: gspE - type II secretion system ATPase GspE, at 4,800,675 to 4,802,156
gspE
LU632_RS25240: gspF - type II secretion system inner membrane protein GspF, at 4,802,146 to 4,803,345
gspF
LU632_RS25245: gspG - type II secretion system major pseudopilin GspG, at 4,803,368 to 4,803,811
gspG
LU632_RS25250: gspI - type II secretion system minor pseudopilin GspI, at 4,803,813 to 4,804,208
gspI
LU632_RS25255: gspJ - type II secretion system minor pseudopilin GspJ, at 4,804,205 to 4,804,756
gspJ
Position (kb)
4802
4803
4804 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4801.151 kb on + strand, within gspE at 4801.166 kb on + strand, within gspE at 4801.169 kb on + strand, within gspE at 4801.170 kb on - strand, within gspE at 4801.206 kb on - strand, within gspE at 4801.258 kb on + strand, within gspE at 4801.258 kb on + strand, within gspE at 4801.258 kb on + strand, within gspE at 4801.259 kb on - strand, within gspE at 4801.259 kb on - strand, within gspE at 4801.259 kb on - strand, within gspE at 4801.493 kb on + strand, within gspE at 4801.522 kb on - strand, within gspE at 4801.522 kb on - strand, within gspE at 4801.712 kb on + strand, within gspE at 4801.713 kb on - strand, within gspE at 4802.152 kb on + strand at 4802.153 kb on - strand at 4802.154 kb on + strand at 4802.155 kb on - strand at 4802.155 kb on - strand at 4802.155 kb on - strand at 4802.155 kb on - strand at 4802.315 kb on + strand, within gspF at 4802.538 kb on - strand, within gspF at 4802.622 kb on + strand, within gspF at 4802.623 kb on - strand, within gspF at 4802.935 kb on - strand, within gspF at 4802.935 kb on - strand, within gspF at 4803.034 kb on + strand, within gspF at 4803.035 kb on - strand, within gspF at 4803.035 kb on - strand, within gspF at 4803.035 kb on - strand, within gspF at 4803.072 kb on - strand, within gspF at 4803.072 kb on - strand, within gspF at 4803.073 kb on + strand, within gspF at 4803.073 kb on + strand, within gspF at 4803.331 kb on - strand at 4803.333 kb on + strand at 4803.333 kb on + strand at 4803.333 kb on + strand at 4803.333 kb on + strand at 4803.333 kb on + strand at 4803.338 kb on + strand at 4803.351 kb on + strand at 4803.351 kb on + strand at 4803.360 kb on + strand at 4803.361 kb on - strand at 4803.368 kb on - strand at 4803.429 kb on + strand, within gspG at 4803.443 kb on - strand, within gspG at 4803.466 kb on + strand, within gspG at 4803.529 kb on + strand, within gspG at 4803.530 kb on - strand, within gspG at 4803.530 kb on - strand, within gspG at 4803.554 kb on + strand, within gspG at 4803.554 kb on + strand, within gspG at 4803.554 kb on + strand, within gspG at 4803.555 kb on - strand, within gspG at 4803.644 kb on + strand, within gspG at 4803.659 kb on + strand, within gspG at 4803.659 kb on + strand, within gspG at 4803.659 kb on + strand, within gspG at 4803.661 kb on + strand, within gspG at 4803.948 kb on - strand, within gspI at 4803.953 kb on + strand, within gspI at 4803.955 kb on + strand, within gspI at 4803.956 kb on - strand, within gspI at 4803.956 kb on - strand, within gspI at 4803.965 kb on + strand, within gspI at 4804.258 kb on + strand at 4804.287 kb on - strand, within gspJ
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS) remove 4,801,151 + gspE LU632_RS25235 0.32 -0.7 4,801,166 + gspE LU632_RS25235 0.33 -0.5 4,801,169 + gspE LU632_RS25235 0.33 +0.1 4,801,170 - gspE LU632_RS25235 0.33 +1.7 4,801,206 - gspE LU632_RS25235 0.36 +0.9 4,801,258 + gspE LU632_RS25235 0.39 +2.0 4,801,258 + gspE LU632_RS25235 0.39 +2.3 4,801,258 + gspE LU632_RS25235 0.39 -0.6 4,801,259 - gspE LU632_RS25235 0.39 -0.1 4,801,259 - gspE LU632_RS25235 0.39 +0.7 4,801,259 - gspE LU632_RS25235 0.39 -2.7 4,801,493 + gspE LU632_RS25235 0.55 +0.4 4,801,522 - gspE LU632_RS25235 0.57 +1.0 4,801,522 - gspE LU632_RS25235 0.57 -0.9 4,801,712 + gspE LU632_RS25235 0.70 -2.1 4,801,713 - gspE LU632_RS25235 0.70 -1.1 4,802,152 + +0.2 4,802,153 - -1.9 4,802,154 + +0.2 4,802,155 - -0.8 4,802,155 - +1.9 4,802,155 - +1.6 4,802,155 - +0.6 4,802,315 + gspF LU632_RS25240 0.14 -0.1 4,802,538 - gspF LU632_RS25240 0.33 +0.8 4,802,622 + gspF LU632_RS25240 0.40 -1.4 4,802,623 - gspF LU632_RS25240 0.40 +0.7 4,802,935 - gspF LU632_RS25240 0.66 -2.0 4,802,935 - gspF LU632_RS25240 0.66 +0.7 4,803,034 + gspF LU632_RS25240 0.74 +1.5 4,803,035 - gspF LU632_RS25240 0.74 +1.2 4,803,035 - gspF LU632_RS25240 0.74 +1.2 4,803,035 - gspF LU632_RS25240 0.74 -0.9 4,803,072 - gspF LU632_RS25240 0.77 +0.5 4,803,072 - gspF LU632_RS25240 0.77 +1.4 4,803,073 + gspF LU632_RS25240 0.77 +1.1 4,803,073 + gspF LU632_RS25240 0.77 +3.2 4,803,331 - -1.3 4,803,333 + +0.7 4,803,333 + +1.6 4,803,333 + -0.2 4,803,333 + -0.4 4,803,333 + +1.0 4,803,338 + +0.2 4,803,351 + +0.2 4,803,351 + +0.4 4,803,360 + +1.8 4,803,361 - -0.1 4,803,368 - +0.5 4,803,429 + gspG LU632_RS25245 0.14 -0.4 4,803,443 - gspG LU632_RS25245 0.17 +0.2 4,803,466 + gspG LU632_RS25245 0.22 -1.1 4,803,529 + gspG LU632_RS25245 0.36 +1.2 4,803,530 - gspG LU632_RS25245 0.36 -0.8 4,803,530 - gspG LU632_RS25245 0.36 +3.2 4,803,554 + gspG LU632_RS25245 0.42 -2.1 4,803,554 + gspG LU632_RS25245 0.42 -1.8 4,803,554 + gspG LU632_RS25245 0.42 +1.0 4,803,555 - gspG LU632_RS25245 0.42 -1.6 4,803,644 + gspG LU632_RS25245 0.62 -2.1 4,803,659 + gspG LU632_RS25245 0.66 +0.0 4,803,659 + gspG LU632_RS25245 0.66 +1.4 4,803,659 + gspG LU632_RS25245 0.66 +1.1 4,803,661 + gspG LU632_RS25245 0.66 +0.7 4,803,948 - gspI LU632_RS25250 0.34 +1.2 4,803,953 + gspI LU632_RS25250 0.35 -0.4 4,803,955 + gspI LU632_RS25250 0.36 -1.1 4,803,956 - gspI LU632_RS25250 0.36 -0.1 4,803,956 - gspI LU632_RS25250 0.36 +0.6 4,803,965 + gspI LU632_RS25250 0.38 +3.3 4,804,258 + -0.9 4,804,287 - gspJ LU632_RS25255 0.15 +0.2
Or see this region's nucleotide sequence